本文選題：味連　切入點(diǎn)：雅連　出處：《西南交通大學(xué)》2017年碩士論文

【摘要】：黃連為毛茛科植物黃連Coptischinensis Franch、三角葉黃連Coptis deltoidea C.Y.Cheng et Hsiao.或云連Coptis teeta Wall.的干燥根莖。秋季采挖,除去須根及泥沙,干燥,撞去殘留須根。黃連因基源不同,藥材分為"味連"、"雅連"和"云連"。黃連是一味川產(chǎn)道地藥材,四川是其主產(chǎn)區(qū),主要有味連、雅連,另有四川歷史習(xí)用品種峨眉野連 Coptis omeiensis(Chen)C.Y.Cheng.(習(xí)稱"鳳尾連")。(1)首先建立了同時(shí)測定7種黃連生物堿成分的HPLC含量測定方法,經(jīng)方法學(xué)驗(yàn)證該方法準(zhǔn)確性、重現(xiàn)性、精密度、穩(wěn)定性、線性均符合要求。測定6批味連、6批雅連、3批鳳尾連的7種生物堿的含量以及總生物堿的含量,對比不同種之間總生物堿以及7種生物堿分別的含量差異,并分析3個(gè)品種之間化學(xué)成分的差異。結(jié)果表明:味連的總生物堿含量最高,7種生物堿在味連、雅連、鳳尾連中含量各不相同,差異較大。(2)通過四因素三水平的正交實(shí)驗(yàn),以生物堿總含量為指標(biāo),篩選了酒黃連、姜黃連、萸黃連的最佳炮制工藝;以雅連為例,對比了黃連不同炮制品與生品的總生物堿含量以及7種不同生物堿各自的含量差異。結(jié)果表明,萸黃連炮制后總生物堿含量最高,且高于生品,姜黃連次之,亦高于生品,酒黃連則低于生品;而7種生物堿各自含量,因炮制方法不同而相異。(3)建立了黃連藥材HPLC特征圖譜測定方法,經(jīng)方法學(xué)驗(yàn)證,該方法精密度、重現(xiàn)性、穩(wěn)定性均符合要求;通過對15批黃連的特征圖譜相似度的分析,找出共有特征峰15個(gè),與黃連生物堿對照品比對后,指認(rèn)了 8個(gè)峰,均為黃連生物堿成分。(4)采用四氧嘧啶小鼠糖尿病模型,對比了味連、雅連及其炮制品在降血糖方面的藥效差異,結(jié)果表明,在降血糖方面各不同基源黃連與其不同炮制品效果差異不大,以萸雅連效果最為明顯,在調(diào)節(jié)糖脂代謝紊亂和抗氧化方面,以雅連效果最佳。
[Abstract]:Rhizoma Coptidis is the dried rhizome of Coptischinensis Franch, Coptis deltoidea C.Y.Cheng et Hsiao. or Coptis teeta Wall. in autumn. The medicinal materials are divided into "Weilian", "Yalen" and "Yunlian". Coptis is a medicinal material produced in Sichuan Province, and Sichuan is the main region of production. In addition, a HPLC method for the simultaneous determination of alkaloids of 7 species of Coptis chinensis was established. The method was proved to be accurate, reproducible, precise and stable by using the traditional Sichuan variety Coptis omeiensis Coptis C.Y.Cheng.At first, the method was used to determine the alkaloids of 7 species of Coptis chinensis. The contents of 7 kinds of alkaloids and total alkaloids of 6 batches of Yalantii and 3 batches of Acanthopsis chinensis were determined, and the differences of total alkaloids and 7 kinds of alkaloids between different species were compared. The results showed that the contents of seven alkaloids were the highest among the three cultivars, and the contents of seven alkaloids were different from each other, and the differences were significant. 2) through the orthogonal experiments of four factors and three levels, the content of total alkaloids was the highest, and the content of seven alkaloids was different among the three varieties. Taking the total alkaloid content as the index, the optimum processing technology of Coptis chinensis, Rhizoma Corni and Coptis officinalis was screened. The contents of total alkaloids and seven kinds of alkaloids in different processed and raw products of Coptis chinensis were compared. The results showed that the content of total alkaloids of Cornel was the highest and higher than that of raw products, followed by Rhizoma Coptidis, and higher than that of raw ones. The content of 7 alkaloids, which were different from each other by different processing methods, was determined by HPLC characteristic chromatogram. The method was proved to be accurate, reproducible and stable. By analyzing the similarity of characteristic atlas of 15 batches of Coptis chinensis, 15 common characteristic peaks were found. After comparing with alkaloids of Coptis chinensis, 8 peaks were identified, all of which were alkaloid components of Coptis chinensis. The results showed that there was no significant difference in the effect of different basic sources of Rhizoma Coptidis and its processed products in lowering blood glucose, especially the effect of Corni's Artemisia officinalis was the most obvious, compared with its processed products, and the results showed that there was no significant difference in the effect of Rhizoma Coptidis and its processed products. Yalen has the best effect in regulating the disorder of glucose and lipid metabolism and anti-oxidation.
【學(xué)位授予單位】：西南交通大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R284;R285.5

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