本文選題：喉鱗狀細(xì)胞癌　切入點：脂肪酸代謝　出處：《廣西醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：研究背景喉癌是頭頸部最常見的惡性腫瘤之一,其中絕大多數(shù)為喉鱗狀細(xì)胞癌(Laryngeal squamous cell carcinoma,LSCC),近年來其發(fā)病率呈上升趨勢,其發(fā)病機制尚不甚清楚,因此學(xué)者們致力于探索喉癌發(fā)生發(fā)展機制。腫瘤細(xì)胞能量代謝改變?nèi)鏦arburg效應(yīng)在腫瘤增殖、調(diào)亡、轉(zhuǎn)移等方面發(fā)揮重要作用。脂肪酸是能量代謝的重要底物,腫瘤細(xì)胞中脂肪酸代謝呈異常激活狀態(tài),脂肪酸代謝相關(guān)基因表達(dá)水平的改變可能影響腫瘤細(xì)胞的生物學(xué)行為。研究目的為進(jìn)一步探討喉鱗狀細(xì)胞癌中脂肪酸代謝機制,我們開展本課題研究即脂肪酸代謝相關(guān)基因脂肪相關(guān)分化蛋白(Adipose differentiation-related protein,ADRP)和乙酰輔酶A酰基轉(zhuǎn)移酶(Acetyl CoA acyltransferase 1,ACAA1)基因在LSCC中的表達(dá)特點,分析其表達(dá)與臨床病理參數(shù)的關(guān)系,為進(jìn)一步的實驗研究提供理論基礎(chǔ)。研究方法1.通過實時熒光定量PCR方法檢測LSCC中的ADRP和ACAA1基因的轉(zhuǎn)錄水平。2.通過用免疫組織化學(xué)染色法從蛋白水平檢測LSCC中ADRP和ACAA1的異常表達(dá)。3.結(jié)合LSCC樣本的臨床特征,分析脂肪酸代謝基因ADRP和ACAA1與喉癌發(fā)生發(fā)展的關(guān)系。研究結(jié)果1.檢測發(fā)現(xiàn)相對于癌旁組織,ADRP和ACAA1在LSCC中轉(zhuǎn)錄水平下調(diào)。2.LSCC中ADRP和ACAA1的蛋白水平呈明顯表達(dá)下調(diào)。3.ADRP和ACAA1的表達(dá)與LSCC的TNM分期、分化程度有關(guān)。研究結(jié)論脂肪酸代謝相關(guān)基因ADRP和ACAA1在LSCC中異常表達(dá),此外與LSCC的TNM分期、病理分級有關(guān),提示了ADRP和ACAA1的異常表達(dá)可能參與LSCC的發(fā)生發(fā)展過程,為探索LSCC的發(fā)生機制提供新的思路。
[Abstract]:Background laryngeal carcinoma is one of the most common malignant tumors in the head and neck, most of which are laryngeal squamous cell carcinomas. Therefore, scholars have been working to explore the mechanism of laryngeal cancer. Changes in energy metabolism of tumor cells, such as Warburg effect, play an important role in tumor proliferation, apoptosis, metastasis and so on. Fatty acids are important substrates of energy metabolism. Fatty acid metabolism is abnormally activated in tumor cells. The changes of fatty acid metabolism related gene expression may affect the biological behavior of tumor cells. The purpose of this study was to further explore the mechanism of fatty acid metabolism in laryngeal squamous cell carcinoma (LSCC). In this study, we studied the expression of adipose differentiation-related protein (ADRP) and Acetyl CoA acyltransferase 1 (ACAA1) gene in LSCC, and analyzed the relationship between the expression and clinicopathologic parameters. To provide a theoretical basis for further experimental research. 1. Detection of the transcription level of ADRP and ACAA1 genes in LSCC by real-time fluorescent quantitative PCR. 2. Detection of ADRP in LSCC by immunohistochemical staining from protein level. And abnormal expression of ACAA1. 3. Combined with the clinical characteristics of LSCC samples, To analyze the relationship between fatty acid metabolism gene ADRP and ACAA1 and the occurrence and development of laryngeal carcinoma. 1. The expression of ADRP and ACAA1 protein was down-regulated in LSCC compared with that of adRP and ACAA1 in paracancerous tissue. 2. The expression of ADRP and ACAA1 in LSCC was down-regulated. The expression of ACAA1 was associated with the TNM staging of LSCC. Conclusion the abnormal expression of fatty acid metabolism-related genes ADRP and ACAA1 in LSCC is related to the TNM stage and pathological grade of LSCC, suggesting that the abnormal expression of ADRP and ACAA1 may be involved in the occurrence and development of LSCC. It provides a new idea for exploring the mechanism of LSCC.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R739.65

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