本文選題：母孕期應(yīng)激　切入點(diǎn)：單一延長(zhǎng)應(yīng)激　出處：《河北醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:觀察不同孕期經(jīng)歷應(yīng)激對(duì)成年子代大鼠認(rèn)知及情緒功能的影響。方法:將16只健康清潔級(jí)Sprague Dawley(SD)雌性大鼠隨機(jī)分成4組:對(duì)照組(CON)、孕早期組(PS1,妊娠第1-7d)、孕晚期組(PS3,妊娠第15-21d)、孕早期組+孕晚期組(PS1-3,妊娠第1-7d聯(lián)合15-21d),每組各4只。PS1、PS3、PS1-3組分別在妊娠第7d、第15d、第7d和第15 d給予單一延長(zhǎng)刺激(single-prolonged stress,SPS),對(duì)照組在妊娠期不給予任何處理。第8w末采用蔗糖偏好、強(qiáng)迫游泳、高架十字迷宮及Morris水迷宮試驗(yàn)分別評(píng)價(jià)其抑郁、焦慮和認(rèn)知功能。實(shí)驗(yàn)數(shù)據(jù)采用SPSS21.0進(jìn)行統(tǒng)計(jì)學(xué)分析。蔗糖偏好率(G,%)、強(qiáng)迫游泳后4分鐘不動(dòng)時(shí)間(T4,單位:s)、高架十字迷宮中進(jìn)入開臂的次數(shù)及時(shí)間(s)、水迷宮前5天平均逃避潛伏期(s)、第6天的穿越平臺(tái)象限時(shí)間(s)及次數(shù)數(shù)據(jù)以均數(shù)±標(biāo)準(zhǔn)差(sx?)表示。檢驗(yàn)水準(zhǔn)為0.05。對(duì)蔗糖偏好率、強(qiáng)迫游泳后4分鐘靜止不動(dòng)時(shí)間(T4,單位:s)、高架十字迷宮中進(jìn)入開臂的次數(shù)及時(shí)間(s)進(jìn)行單因素方差分析和Bonferroni法兩兩比較,水迷宮前5天大鼠的平均逃避潛伏期進(jìn)行重復(fù)測(cè)量分析。水迷宮第6天大鼠穿越平臺(tái)象限時(shí)間(單位:s)及次數(shù)采用進(jìn)行單因素方差分析和Bonferroni法兩兩比較。結(jié)果:1在蔗糖偏愛試驗(yàn)中,不同孕期應(yīng)激組(F=52.597,P=0.000)及性別(F=5.931,P=0.018)均有統(tǒng)計(jì)學(xué)差異。兩兩比較的結(jié)果顯示,PS3的蔗糖偏愛率比CON組低而比PS1高。同性別之間比較,雌性子代:PS3的蔗糖偏愛率比CON組低而比PS1高,差異有統(tǒng)計(jì)學(xué)意義(P0.01);雄性子代:PS3的蔗糖偏愛率比CON組低而比PS1高,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。性別之間比較:PS1、PS1-3及CON雌雄之間均無(wú)差異(P0.01),PS3組雌性蔗糖偏愛率比PS3組雄性低,且差異有統(tǒng)計(jì)學(xué)意義(P0.01)。PS1-3組與對(duì)照組無(wú)統(tǒng)計(jì)學(xué)意義(P0.01)。2比較強(qiáng)迫游泳后4min的不動(dòng)時(shí)間,不同孕期應(yīng)激組(f=17.287,p=0.000)及性別(f=4.745,p=0.033)均有統(tǒng)計(jì)學(xué)差異。兩兩比較的結(jié)果顯示,ps3在后4min的不動(dòng)時(shí)間高于con而ps1及ps1-3低于con。同性別之間比較,雌性子代:ps3在后4min的不動(dòng)時(shí)間高于con,而ps1及ps1-3低于con,差異有統(tǒng)計(jì)學(xué)意義(p0.01);雄性子代:ps3與con相比在后4min的不動(dòng)時(shí)間增加而ps1及ps1-3降低,差異有統(tǒng)計(jì)學(xué)意義(p0.01)。性別之間比較:ps1、ps1-3及con雌雄之間均無(wú)差異(p0.01),ps3組雌性后4min的不動(dòng)時(shí)間比ps3組雄性增加,且差異有統(tǒng)計(jì)學(xué)意義(p0.01)。3在高架十字迷宮實(shí)驗(yàn)中,于開臂中停留的時(shí)間中,不同孕期應(yīng)激組(f=5.326,p=0.002)及性別(f=11.638,p=0.001)均有統(tǒng)計(jì)學(xué)差異。兩兩比較的結(jié)果顯示,孕期應(yīng)激三組均比con減少且差異有統(tǒng)計(jì)學(xué)意義(p0.01),而孕期應(yīng)激三組之間相比無(wú)統(tǒng)計(jì)學(xué)意義(p0.01)。孕晚期應(yīng)激組的雌性與雄性相比減少且差異有統(tǒng)計(jì)學(xué)意義(p0.01)。在進(jìn)入開臂的次數(shù)中,不同孕期應(yīng)激組(f=13.216,p=0.000)有統(tǒng)計(jì)學(xué)差異而性別(f=1.126,p=0.293)無(wú)統(tǒng)計(jì)學(xué)差異,ps1及ps3組較con進(jìn)入開臂的次數(shù)減少有統(tǒng)計(jì)學(xué)差異(p0.05)。4水迷宮測(cè)試中,在前5天,不同孕期應(yīng)激組(f=10.653p=0.000)及時(shí)間(f=82.068,p=0.000)均有統(tǒng)計(jì)學(xué)差異。但是性別之間沒有統(tǒng)計(jì)學(xué)差異(f=0.000,p=0.100)。多變量方差分析顯示:水迷宮第1d,在逃避潛伏期上,ps3和ps1-3有輕微的減少而ps1有輕微的增加,但是均沒有統(tǒng)計(jì)學(xué)意義(f=1.963,p0.05),水迷宮2~5天,各組之間el差異均有統(tǒng)計(jì)學(xué)意義(f分別為5.057,4.463,11.052,10.245,p0.05),其中第2天,ps3及ps1-3的el低于對(duì)照組;第3天,僅ps1-3組el低于對(duì)照組;第4天、5天,ps1的el高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(p0.01)。第6天,經(jīng)單因素方差分析,結(jié)果顯示四組間穿越平臺(tái)象限的時(shí)間及次數(shù)均有統(tǒng)計(jì)學(xué)意義(f分別為6.801,9.014,p0.01),但是性別之間沒有統(tǒng)計(jì)學(xué)意義(p分別為0.173,0.036,f0.05)。兩兩比較bonferroni分析顯示,在穿越平臺(tái)象限時(shí)間及次數(shù)中,ps3組均高于對(duì)照組(p0.05)。結(jié)論:不同孕期應(yīng)激對(duì)子代成年后情緒及認(rèn)知功能影響不同。1孕早期經(jīng)歷應(yīng)激能夠降低抑郁樣行為的出現(xiàn),引起焦慮行為的產(chǎn)生,損害后代學(xué)習(xí)能力。2孕晚期經(jīng)歷應(yīng)激能夠?qū)е乱钟魳有袨榈某霈F(xiàn),引起焦慮行為的產(chǎn)生,促進(jìn)后代學(xué)習(xí)記憶能力。3孕早期聯(lián)合孕晚期經(jīng)歷應(yīng)激對(duì)成年后代的情緒及認(rèn)知功能影響不大。4性別之間,只有孕晚期經(jīng)歷應(yīng)激的成年雌性后代更易出現(xiàn)焦慮抑郁樣行為。
[Abstract]:Objective: To observe the different effects of stress on pregnancy experience in adult offspring rats cognitive and emotional function. Methods: 16 healthy Sprague Dawley (SD) female rats were randomly divided into 4 groups: control group (CON), early pregnancy group (PS1, 1-7d of pregnancy, late pregnancy group (PS3). 15-21d of pregnancy), early pregnancy group and late pregnancy group (PS1-3, 1-7d of pregnancy combined with 15-21d), 4 rats in each group.PS1, PS3, PS1-3 group respectively in the 7d of pregnancy, 15d, 7d and d give fifteenth single (single-prolonged stress, SPS prolonged stimulation), the control group not given during pregnancy any treatment. At the end of 8W by sucrose preference, forced swimming, elevated plus maze and Morris water maze test were evaluated for depression, anxiety and cognitive function. The experimental data were statistically analyzed by SPSS21.0. The sucrose preference rate (G,%), 4 minutes after the forced swimming immobility time (T4, unit: s). The elevated plus maze into the open The number of arm water maze (s), 5 days before the average escape latency (s), sixth days (s) through the platform quadrant time and frequency data to mean + standard deviation (SX?). The test level of 0.05. on the rate of sucrose preference, forced swimming for 4 minutes after the immobility time (T4: s), elevated plus maze in the open arm of the times and time (s) were compared with single factor variance analysis and Bonferroni method 22, 5 days before the water maze rats the average escape latency for repeated measurement analysis. Water maze rats sixth days through the platform quadrant time (unit: S) and the number of times were compared using one-way ANOVA and Bonferroni method. Results: 1 in 22 sucrose preference test in different pregnancy stress group (F=52.597, P=0.000) and gender (F=5.931, P=0.018) were statistically significant difference. 22 comparison results show that PS3 sucrose preference rate lower than CON group and higher than PS1. The same Comparison between the sexes, female offspring: PS3 sucrose preference rate lower than CON group and higher than PS1, the difference was statistically significant (P0.01); male offspring: PS3 sucrose preference rate lower than CON group and higher than PS1, the difference was statistically significant (P0.01). The comparison between gender: PS1, PS1-3 and there were no differences in CON and PS3 group (P0.01), sucrose preference rate of PS3 group than in female male is low, and the difference was statistically significant (P0.01) in.PS1-3 group and control group had no statistical significance (P0.01).2 4min forced swimming immobility time in different pregnancy stress group (f= 17.287, p=0.000) and gender (f=4.745, p=0.033) were statistically significant difference. 22 comparison results show that PS3 in 4min after the real time higher than con and PS1 and ps1-3 lower than the comparison between con. and sex, female offspring: PS3 in 4min after the real time higher than that of con, PS1 and ps1-3 is lower than con, the difference was statistically significant (P0.01 male); Offspring: PS3 compared with con in 4min after the real time increased while PS1 and ps1-3 decreased, the difference was statistically significant (P0.01). The gender comparison between PS1, ps1-3 and con between male and female were no difference (P0.01), PS3 group of female 4min after the immobility time in PS3 group than in the male, and the poor there was a significant correlation (P0.01).3 in the elevated plus maze, to open arms stay in different pregnancy stress group (f=5.326, p=0.002) and gender (f=11.638, p=0.001) were statistically significant difference. 22 comparison results show that prenatal stress in three groups were less than in con and the difference was statistically significant (P0.01), and between prenatal stress compared to the three groups had no statistical significance (P0.01). The male and female decreased and the difference was statistically significant compared with late pregnancy stress group (P0.01). The number of open arm in different pregnancy stress group (f =13.216, p=0.000) and gender difference (f=1 .126, p=0.293) had no significant difference, PS1 and PS3 compared with group con in open arms reductions in the number of statistically significant (P0.05).4 water maze test, 5 days ago, during different stress group (f=10.653p=0.000) and time (f=82.068, p=0.000) were statistically significant. But there were no statistical differences between genders (f=0.000, p=0.100). Multivariate analysis of variance showed that the 1D water maze test, the escape latency in PS3, and ps1-3 and PS1 have a slight increase decrease slightly, but there was no statistically significant difference (f=1.963, P0.05), 2~5 water maze, there were statistically significant differences between groups el (F = 5.057,4.463,11.052,10.245, P0.05 in second days, PS3), and ps1-3 El lower than the control group; the third day, only ps1-3 group El was lower than the control group; fourth days, 5 days, PS1 El was higher than the control group, the difference was statistically significant (P0.01). The sixth day, by the single factor analysis of variance, the results showed that the four groups Cross platform quadrant time and frequency were statistically significant (F = 6.801,9.014, P0.01), but there was no statistical significance between the sexes (P = 0.173,0.036, f0.05). 22 Bonferroni analysis showed that, in the time and frequency of passing through the platform quadrant in PS3 group were higher than control group (P0.05). Conclusion: different pregnancy stress in offspring in adulthood affect mood and cognitive function of different.1 in early pregnancy experience stress can reduce the anxiety caused by depression like behavior, behavior, damage to the offspring of the learning ability of.2 in late pregnancy experience can lead to stress depression like behavior, anxiety behavior, promote the ability of learning and memory of.3 offspring in early pregnancy and pregnancy advanced experience stress on adult offspring emotion and cognitive function has little effect on.4 sex between adult female offspring than in late pregnancy experience stress more prone to anxiety and depression Like behavior.

【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R749

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