本文關(guān)鍵詞：紫蘇葉提取物的降血糖活性研究　出處：《浙江大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 紫蘇葉提取物 α-葡萄糖苷酶 降血糖 跨膜轉(zhuǎn)運

【摘要】：近年來,隨著社會的發(fā)展和人們生活方式的改變,糖尿病的發(fā)病率在全球范圍內(nèi)呈上升的趨勢,嚴(yán)重威脅著人類的健康。目前主要采用降血糖藥物和注射胰島素的方式治療,但會引發(fā)一些副作用,所以開發(fā)利用天然的抗糖尿病藥物顯得尤為必要。紫蘇在我國已有2000多年的栽培歷史,主產(chǎn)于貴州、江蘇、安徽、湖南等地,具有極為廣泛的資源。近些年來,紫蘇葉因其特有的活性物質(zhì)及營養(yǎng)成分,成為一種倍受世界關(guān)注的多用途植物,經(jīng)濟(jì)價值很高。研究表明,紫蘇葉提取物能在糖尿病綜合癥治療中起到重要作用,但是目前關(guān)于發(fā)揮這些功效的活性組分及其作用機(jī)理尚無明確的定論。本課題對紫蘇葉提取物的降血糖作用和相關(guān)機(jī)理進(jìn)行了初步的研究。本文首先探討了紫蘇葉的熱水浸提工藝,通過單因素實驗和響應(yīng)面實驗來研究浸提溫度、浸提時間、料液比、浸提次數(shù)等因素對紫蘇葉中降血糖活性成分得率的影響,以確定紫蘇葉中降血糖活性成分最佳的浸提工藝。結(jié)果表明在浸提溫度為52℃,浸提時間為60min,料液比為1:25,浸提次數(shù)為3次時,紫蘇葉中降血糖活性成分的得率最高。其次,比較了紫蘇葉提取物和阿卡波糖對不同來源的α-葡萄糖苷酶的抑制作用,結(jié)果表明紫蘇葉提取物對豬胰腸來源的α-葡萄糖苷酶、大米來源的α-葡萄糖苷酶及酵母來源的α-葡萄糖苷酶有較好的抑制作用,對細(xì)菌來源的α-葡萄糖苷酶沒有表現(xiàn)出抑制活性。在此基礎(chǔ)上,測定了紫蘇葉提取物對不同來源的α-葡萄糖苷酶的抑制類型,實驗結(jié)果反應(yīng)紫蘇葉提取物對豬胰腸來源的α-葡萄糖苷酶、酵母來源的α-葡萄糖苷酶和大米來源的α-葡萄糖苷酶的抑制類型均屬于競爭性抑制類型。最后,采用了 Caco-2細(xì)胞單層模型來考察樣品對小腸中葡萄糖轉(zhuǎn)運的影響,通過考察紫蘇葉提取物對Caco-2細(xì)胞上雙糖酶活力的影響以及紫蘇葉提取物對葡萄糖在Caco-2細(xì)胞中轉(zhuǎn)運的抑制情況來探討紫蘇葉提取物降血糖機(jī)制。結(jié)果表明紫蘇葉提取物對Caco-2細(xì)胞上的麥芽糖酶和蔗糖酶有良好的抑制作用,并且對葡萄糖的轉(zhuǎn)運有一定的抑制作用。
[Abstract]:In recent years, with the development of society and the change of people's life style, the incidence of diabetes is on the rise in the global scope. It is a serious threat to human health. Currently, it is mainly treated with hypoglycemic drugs and insulin injections, but it can cause some side effects. Therefore, the development and utilization of natural anti-diabetic drugs is particularly necessary. Perilla has been cultivated in China for more than 2000 years, mainly produced in Guizhou, Jiangsu, Anhui, Hunan and other places. In recent years, perilla leaf has become a kind of multi-purpose plant which has attracted worldwide attention because of its unique active substances and nutrients, and its economic value is very high. Perilla leaf extract can play an important role in the treatment of diabetic syndrome. However, there is no definite conclusion about the active components and their mechanism. The hypoglycemic effect and related mechanism of perilla leaf extract were studied in this paper. Firstly, the perilla perilla was discussed in this paper. Hot water extraction of leaves. Single factor experiment and response surface experiment were used to study the effects of extraction temperature, extraction time, ratio of material to liquid and times of extraction on the yield of hypoglycemic components in perilla perilla leaves. The results showed that the extraction temperature was 52 鈩，

本文編號：1374076