發(fā)布時間：2017-12-28 23:17

  本文關鍵詞：電針促脂肪代謝的腺苷受體機制　出處：《南京中醫(yī)藥大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 電針 肥胖 腺苷受體 白色脂肪組織 脂肪代謝 能量消耗

【摘要】：目的:觀察電針對高脂飲食誘導肥胖(diet induced obesity,DIO)小鼠白色脂肪組織(WAT)中腺苷受體的影響,探討腺苷受體在電針減肥中的作用機制。方法:將63只3周齡雄性C57BL/6小鼠隨機分為普食組(21只)和高脂組(42只),分別給予普通飲食和高脂飲食喂養(yǎng)12周后,以體質量超過正常小鼠平均體質量的20%作為肥胖模型標準,并最終將小鼠隨機分為正常對照組(8只)、正常電針組(13只)、模型對照組(9只)、模型電針組(14只)。兩個治療組均電針"后三里"和"內(nèi)庭"穴,每周治療6次,共電針4周。測量小鼠體質量和附睪脂肪組織質量,計算小鼠體重變化以及脂肪組織脂體比;用代謝籠監(jiān)測小鼠熱量釋放、攝食、活動量(XT+YT)和總移動路程;HE染色評價脂肪細胞的體積;用qPCR技術檢測脂肪組織中A1R、A2AR、A2BR、A3R的mRNA表達以及棕色化相關基因Tbx1、Tmem26的表達;用Western Blot檢測脂肪組織中腺苷受體、PRDM16、PGC-1α、UCP1、pTH、pHSL 的蛋白表達。結果:實驗一:1.與普食組相比,高脂喂養(yǎng)12周能明顯增加小鼠體質量(P0.01),其中,體質量超過普食組20%的小鼠有23只(占54.76%)。2.治療結束后,與正常對照組相比,正常電針組小鼠體質量和附睪脂肪組織(Epi-WAT)脂體比下降(P0.05),模型對照組體質量依然維持在較高狀態(tài)(P0.05)、體質量變化值差異有統(tǒng)計學意義(P0.01)、Epi-WAT質量和脂體比升高(P0.01,P0.05);與模型對照組相比,模型電針組體質量、Epi-WAT脂體比均降低(P0.05)、體質量變化值增大(P0.01)。3.代謝籠:與正常對照組相比,模型對照組VO2水平下降(P0.05)、呼吸交換率(Respiratory exchange rate,RER)升高(P0.05)、攝食量升高(P0.05)、活動量(XT+YT)和總移動路程無顯著差異(P0.05);與模型對照組相比,模型電針組H1、H2、V02、VC02水平均升高(P0.05),RER無統(tǒng)計學差異(P0.05),攝食量下降(P0.05),XT+YT、總移動路程無顯著差異(P0.05)。4.HE染色:與正常對照組相比,正常電針組附睪脂肪細胞面積減小(P0.05),模型對照組面積增大(P0.01);與模型對照組相比,模型電針組面積減小(P0.01)。實驗二:1.A1RmRNA在四組中表達的差異無統(tǒng)計學意義(P0.05);與正常對照組相比,正常電針組A2AR和A2BR蛋白表達上調(diào)(P0.05,P0.01),模型對照組A3R mRNA的表達降低(P0.01)、A2AR蛋白表達降低(P0.05)、A2BR蛋白表達差異不明顯(P0.05);與模型對照組相比,模型電針組A2AR、A2BR mRNA表達升高(P0.05),A2AR和A2BR蛋白表達升高(P0.01)。2.與正常對照組相比,模型對照組Tbx1、Tmem26 mRNA表達降低(P0.05),BAT中PRDM16蛋白水平下降(P0.05);與模型對照組相比,模型電針組Tbx1、Tmem26mRNA水平上調(diào)(P0.05),BAT中PRDM16、UCP1蛋白水平上調(diào)(P0.01),Epi-WAT 中 PRDM16、PGC-1α、UCP1 的蛋白表達升高(P0.05,P0.05,P0.01)。3.與正常對照組相比,正常電針組pTH蛋白表達升高(P0.05),模型對照組pTH、pHSL蛋白水平下降(P0.01,P0.05);與模型對照組相比,模型電針組pTH、pHSL蛋白水平均升高(P0.01)。結論:電針可能通過激活自主神經(jīng)系統(tǒng),調(diào)節(jié)WAT中A2AR、A2BR的表達,促進脂肪代謝和能量消耗,這可能是電針減肥的部分機制。
[Abstract]:Objective: To observe the effect of Electroacupuncture on adenosine receptor in white adipose tissue (WAT) of diet induced obesity (DIO) mice induced by high-fat diet, and to explore the mechanism of adenosine receptor in reducing weight by electroacupuncture. Methods: 63 3 week old male C57BL/6 mice were randomly divided into normal control group (21 rats) and high-fat group (42 rats), which were fed with normal diet and high-fat diet for 12 weeks, with the body mass of more than 20% of the average body weight of normal mice as obesity model standard, and finally the mice were divided into normal control group (8 rats), normal acupuncture group (13 rats), model group (9 rats), model group (14 rats) electroacupuncture. The two treatment groups were treated with electroacupuncture "after three li" and "inner court", 6 times a week for 4 weeks. Measurement of mice body weight and epididymal adipose tissue mass, calculate the weight changes of mice adipose tissue and body fat ratio; release, using metabolic cages of mice feeding and activity monitoring heat capacity (XT+YT) and the total moving distance; HE staining was used to evaluate the size of fat cells; using qPCR to detect the fat tissue of A1R, A2AR, A2BR, A3R the expression of mRNA and browning related gene Tbx1 and Tmem26 expression; expression of adenosine Blot receptors in the detection of adipose tissue Western, PRDM16, PGC-1, UCP1, pTH, alpha pHSL protein. Results: Experiment 1: 1., compared with the common food group, high fat diet can significantly increase body weight (P0.01) in 12 weeks, and the body weight of the mice is 20% higher than that of the common food group (23) (54.76%). 2. after the end of treatment, compared with the normal control group, normal acupuncture group of mice body weight and epididymal adipose tissue (Epi-WAT) decreased body fat (P0.05), model control group, the body mass is still maintained at a high state (P0.05), the mass variation value difference was statistically significant (P0.01), Epi-WAT quality and ratio of fat. Increased (P0.01, P0.05); compared with the model group, EA group model body mass, fat body were lower than Epi-WAT (P0.05), the changes of body weight increases (P0.01). 3. metabolic cages: compared with normal control group, model control group, VO2 decreased (P0.05), respiratory exchange ratio (Respiratory exchange rate, RER) (P0.05), increased food intake increased (P0.05), activity (XT+YT) and no significant difference between the total mobile distance (P0.05); compared with the model control group model H1, H2, V02 EA group, the level of VC02 increased (P0.05), RER (P0.05), there was no significant difference in food intake decreased (P0.05), XT+YT had no significant difference, the total mobile distance (P0.05). 4.HE staining: compared with the normal control group, the area of epididymal adipocytes in the normal electroacupuncture group decreased (P0.05), and the area in the model control group increased (P0.01). Compared with the model control group, the area of the EA group decreased (P0.01). Experiment two: there was no significant difference in the expression of 1.A1RmRNA in the four groups (P0.05); compared with the normal control group, normal acupuncture group of A2AR and A2BR protein expression (P0.05, P0.01), model control group A3R expression decreased mRNA (P0.01), lower expression of A2AR (P0.05) and A2BR protein expression difference obviously (P0.05); compared with the model group, the increased expression of A2AR, A2BR, mRNA model EA group (P0.05), the expression of A2AR and A2BR protein level (P0.01). 2. compared with normal control group, model control group Tbx1, Tmem26 decreased expression of mRNA (P0.05), PRDM16 protein level in BAT decreased (P0.05); compared with the model control group, model group, Tbx1 EA increased level of Tmem26mRNA (P0.05), the level of PRDM16 and UCP1 protein in BAT (P0.01), increased expression of Epi-WAT in PRDM16 PGC-1, alpha, UCP1 protein level (P0.05, P0.05, P0.01). 3., compared with the normal control group, the expression of pTH protein in the normal electroacupuncture group increased (P0.05), and the pTH and pHSL protein levels in the model control group decreased (P0.01, P0.05). Compared with the model control group, the pTH and pHSL protein levels in the EA group increased (P0.01). Conclusion: EA may regulate the expression of A2AR and A2BR in WAT and promote lipid metabolism and energy consumption by activating the autonomic nervous system, which may be a part of the mechanism of electro acupuncture for weight loss.
【學位授予單位】：南京中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R245

【相似文獻】

相關期刊論文 前9條

1 萬巧云,高永,曲巍;煙、茶和放射線聯(lián)合作用對小鼠體質量及白細胞的影響(英文)[J];中國臨床康復;2004年27期

2 吳志嵩;路新國;;山楂山藥湯對高脂血癥小鼠體質量和血脂水平的影響[J];西部中醫(yī)藥;2012年02期

3 袁雪梅;黃輝;徐小林;諸源江;張科學;徐佳;陳銀寶;戴體俊;;羥丁酸鈉對小鼠體質量、自主活動和學習記憶的影響[J];中國醫(yī)院藥學雜志;2010年21期

4 孫延雙;陳思凡;朱偉;鄭琳;張子麗;凌文華;馮翔;;白藜蘆醇對KKAy小鼠體質量和脂肪分布的影響及機制[J];食品科學;2011年13期

5 朱超林;王居祥;王瑞平;戴虹;楊晨;;克瘤丸對移植性肉瘤S_(180)小鼠體質量、胸腺脾臟質量及指數(shù)的影響[J];山東中醫(yī)雜志;2007年06期

6 劉曼曼;侯風剛;吳琦;呂祥;余濤;陳e，

本文編號：1347838