本文關(guān)鍵詞：基于大數(shù)據(jù)分析的KRT1、KRT10、GSTP1在食管鱗癌中的表達(dá)及意義　出處：《新疆醫(yī)科大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 食管鱗狀細(xì)胞癌 大數(shù)據(jù)分析 角蛋白1 角蛋白10 谷胱甘肽-S-轉(zhuǎn)移酶P1

【摘要】：目的:分析KRT1、KRT10、GSTP1基因在各腫瘤中表達(dá)的大數(shù)據(jù)信息及在哈薩克族食管鱗癌組織中的表達(dá)及臨床意義。方法:通過(guò)www.proteinatlas.org生物學(xué)信息公眾平臺(tái),查詢(xún)KRT1、KRT10、GSTP1基因在各正常人體組織及各腫瘤中的表達(dá)情況;運(yùn)用Western blot技術(shù)對(duì)28對(duì)新鮮食管癌組織及相應(yīng)的癌旁正常組織中的KRT1、KRT10、GSTP1表達(dá)情況進(jìn)行半定量分析;用免疫組織化學(xué)技術(shù)(IHC)對(duì)60對(duì)食管鱗狀細(xì)胞癌組織及癌旁正常食管黏膜組織中的KRT1、KRT10、GSTP1表達(dá)情況進(jìn)行定性檢測(cè),結(jié)合臨床病理資料,對(duì)免疫組化結(jié)果進(jìn)行數(shù)據(jù)分析,并用ROC曲線對(duì)三個(gè)蛋白的診斷價(jià)值進(jìn)行評(píng)估。結(jié)果:1.Western blot結(jié)果顯示KRT1、KRT10、GSTP1三個(gè)蛋白在食管鱗狀細(xì)胞癌組織中表達(dá)高于相應(yīng)癌旁正常組織(P0.001),在哈薩克族與漢族中表達(dá)無(wú)統(tǒng)計(jì)學(xué)差異(P0.05);2.免疫組化結(jié)果顯示KRT1、KRT10、GSTP1在食管鱗狀細(xì)胞癌組織中的表達(dá)均高于癌旁正常組織(P0.001),結(jié)合臨床病理參數(shù)分析,結(jié)果顯示KRT1、KRT10的表達(dá)與食管鱗癌組織的分化程度有關(guān)(P0.05),GSTP1在食管鱗狀細(xì)胞癌中的強(qiáng)陽(yáng)性表達(dá)與患者的年齡、性別、族別、淋巴結(jié)轉(zhuǎn)移與否有關(guān)(P0.05),根據(jù)ROC曲線下面積提示三個(gè)差異蛋白中KRT10蛋白的診斷價(jià)值最高,GSTP1蛋白其次,KRT1蛋白最低,三者均有診斷價(jià)值。結(jié)論:KRT1、KRT10、GSTP1為食管鱗狀細(xì)胞癌的發(fā)生發(fā)展中的重要變化分子,KRT1、KRT10與食管鱗癌的分化有關(guān),可作為食管鱗癌惡性程度判斷的重要指標(biāo),KRT10有望成為食管鱗癌診斷中的特異性分子標(biāo)記物,GSTP1與患者的年齡、性別、族別、淋巴結(jié)轉(zhuǎn)移有關(guān),可對(duì)食管鱗癌的協(xié)助診斷、惡性程度判斷及后期個(gè)體化治療有一定指導(dǎo)意義。
[Abstract]:Objective: to analyze the large data information expressed by KRT1, KRT10 and GSTP1 genes in various tumors and the expression and clinical significance in Kazak's esophageal squamous cell carcinoma. Methods: the www.proteinatlas.org biological information public platform, the query expression of KRT1, KRT10, GSTP1 gene in normal human tissues and tumor; using semi quantitative analysis of 28 pairs of cancer and corresponding fresh esophageal cancer tissues, the expression of GSTP1 Western KRT1, KRT10 blot; immunohistochemistry Chemical Technology (IHC) expression were detected in 60 of esophageal squamous cell carcinoma tissue and normal esophageal mucosa tissues of KRT1, KRT10, GSTP1, combined with clinical data, data analysis on the results of immunohistochemistry, and were evaluated by ROC curve in diagnosis of three proteins. Results: 1.Western blot showed that KRT1, KRT10 and GSTP1 three protein expression in esophageal squamous cell carcinoma tissues than adjacent normal tissues (P0.001), there were no significant difference in Kazak and Han in 2. (P0.05); immunohistochemistry showed that the expression of KRT1, KRT10 and GSTP1 in esophageal squamous cell carcinoma were higher than those in adjacent normal tissues (P0.001), combined with analysis of clinical pathological parameters, the results showed that KRT1, KRT10 expression and differentiation of esophageal squamous cell carcinoma (P0.05), GSTP1 strongly positive in esophageal squamous cell carcinoma and patients' age, gender, nationality, is associated with lymph node metastasis (P0.05), according to the diagnostic value of the area under the ROC curve showed that KRT10 protein three proteins in the highest GSTP1 protein KRT1 protein, secondly, the lowest value of three has diagnosis. Conclusion: KRT1, KRT10, GSTP1 as an important change in the development of molecular differentiation of esophageal squamous cell carcinoma, KRT1, KRT10 and esophageal squamous cell carcinoma, as an important index to judge the malignant degree of esophageal squamous cell carcinoma, KRT10 is expected to become the specific molecular markers for diagnosis of esophageal squamous cell carcinoma, GSTP1 and the age and sex of the patients, nationality and lymph node metastasis of esophageal squamous cell carcinoma, can assist diagnosis and individualized treatment of judging malignant degree and later have a certain significance.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R735.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 Jun-Hui Guo;Guo-Lan Xing;Xin-Hui Fang;Hui-Fang Wu;Bo Zhang;Jin-Zhong Yu;Zong-Min Fan;Li-Dong Wang;;Proteomic profiling of fetal esophageal epithelium, esophageal cancer, and tumor-adjacent esophageal epithelium and immunohistochemical characterization of a representative differential protein, PRX6[J];World Journal of Gastroenterology;2017年08期

2 黃冬云;許文景;徐興祥;張旭東;;細(xì)胞角蛋白在鱗狀細(xì)胞癌、基底細(xì)胞癌及移行細(xì)胞癌診斷中的作用[J];臨床肺科雜志;2016年07期

3 吳彪;余晶晶;王志炎;;食管未分化癌5例臨床病理特點(diǎn)分析[J];現(xiàn)代實(shí)用醫(yī)學(xué);2016年05期

4 張浩亮;吳愷;侯智亮;趙松;;食管鱗狀細(xì)胞癌血漿外泌體腫瘤標(biāo)志物的蛋白質(zhì)組學(xué)篩選[J];中華實(shí)驗(yàn)外科雜志;2016年05期

5 張俊珍;劉_g;姬凱元;楊?yuàn)檴?胡帥鵬;劉學(xué)賢;范瑞文;;小鼠角蛋白10(K10)基因啟動(dòng)子的克隆及活性分析[J];中國(guó)農(nóng)業(yè)科學(xué);2016年09期

6 趙云崗;張?zhí)?谷娟;王廣超;向慧妮;劉瑞敏;晁瑋霞;賈彩云;韓大正;楊文義;馬遠(yuǎn)方;齊義軍;;iTRAQ聯(lián)用串聯(lián)質(zhì)譜鑒定食管鱗狀細(xì)胞癌差異表達(dá)蛋白質(zhì)及蛋白質(zhì)相互作用網(wǎng)絡(luò)[J];中國(guó)生物化學(xué)與分子生物學(xué)報(bào);2016年01期

7 王洪江;王勇濤;王興名;;EphA2表達(dá)水平與食管鱗癌臨床病理特征關(guān)系研究[J];中國(guó)全科醫(yī)學(xué);2015年33期

8 鄧彥超;張海平;孫清超;張瓊;張昌明;;新疆哈薩克民族食管癌淋巴結(jié)轉(zhuǎn)移的血清蛋白質(zhì)組學(xué)研究[J];健康之路;2015年09期

9 張景萍;李卉;孫曉宏;瓦熱斯江·依不拉音;沙亞哈提·別爾克哈之;劉伊寧;李曉苗;來(lái)雯婷;杰別克·瓦提別克;美麗吾爾提·達(dá)吾列提汗;李惠武;;Atp5a1基因在新疆哈薩克族食管癌中的表達(dá)分析[J];新疆醫(yī)科大學(xué)學(xué)報(bào);2015年07期

10 閻景紅;艾尼瓦爾·艾木都拉;郝潔;楊玉剛;包永星;;2009-2012年新疆腫瘤監(jiān)測(cè)點(diǎn)新源縣惡性腫瘤發(fā)病分析[J];新疆醫(yī)科大學(xué)學(xué)報(bào);2014年06期

相關(guān)博士學(xué)位論文 前1條

1 趙佳;食管鱗狀細(xì)胞癌血漿腫瘤標(biāo)記物的蛋白質(zhì)組學(xué)篩選及驗(yàn)證[D];鄭州大學(xué);2016年

相關(guān)碩士學(xué)位論文 前1條

1 吳平香;FBXO8通過(guò)泛素化GSTP1參與結(jié)直腸癌侵襲和轉(zhuǎn)移的分子機(jī)制[D];南方醫(yī)科大學(xué);2015年

，

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