本文關(guān)鍵詞：Wip1在糖尿病視網(wǎng)膜病變前膜中的表達(dá)　出處：《廣西醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： Wip1 視網(wǎng)膜前膜 增生性糖尿病視網(wǎng)膜病變 NF-κB 膠質(zhì)細(xì)胞

【摘要】：目的:通過檢測野生型P53誘導(dǎo)的磷酸酶1(Wild-type p53-induced phosphatase 1,Wip1)在增生性糖尿病視網(wǎng)膜病變前膜中的表達(dá),同時(shí)分析Wip1和核轉(zhuǎn)錄因子κB(nuclear factor kappa-B,NF-κB)、Wip1和膠質(zhì)細(xì)胞在PDR前膜中的定位關(guān)系,初步探討其參與增生性糖尿病視網(wǎng)膜病變前膜形成的機(jī)制。方法:從玻璃體切割術(shù)中獲取患者視網(wǎng)膜前膜組織,取增生性糖尿病視網(wǎng)膜病變患者的前膜作為實(shí)驗(yàn)組(PDR組),取不合并其他眼病患者的特發(fā)性視網(wǎng)膜前膜(idiopathic epiretinal membrane,i ERM)作為對照組(iERM組)。利用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)檢測前膜組織中Wip1的mRNA表達(dá)。用免疫組化及免疫熒光法檢測前膜中Wip1的蛋白表達(dá)。運(yùn)用免疫熒光雙染法觀察Wip1和GFAP(視網(wǎng)膜膠質(zhì)細(xì)胞的標(biāo)記物),以及Wip1和NF-κB的共定位關(guān)系。結(jié)果:共收集26例視網(wǎng)膜前膜組織,其中PDR組17例,iERM組9例。RT-PCR結(jié)果顯示W(wǎng)ip1的mRNA在PDR組中呈高表達(dá),而在iERM組中僅有微弱表達(dá)。免疫組化和免疫熒光結(jié)果均顯示W(wǎng)ip1在PDR組的前膜中有較強(qiáng)的陽性信號。通過免疫熒光雙染發(fā)現(xiàn)Wip1和GFAP、NF-κB在PDR組前膜中的表達(dá)有相關(guān)性。結(jié)論:本研究結(jié)果說明Wip1可能通過其與NF-κB的相互作用參與了增生性糖尿病視網(wǎng)膜病變中纖維血管膜的形成。
[Abstract]:Objective: through the detection of wild type P53 induced phosphatase 1 (Wild-type p53-induced 1 phosphatase, Wip1) expression in proliferative diabetic retinopathy before in the film, and the analysis of the Wip1 and nuclear factor kappa B (nuclear factor kappa-B, NF- K B), relationship between the localization of Wip1 and glial cells in the PDR membrane in the preliminary to explore the mechanism of the participation of proliferative diabetic retinopathy before film formation. Methods: from the patients with epiretinal membrane cutting obtain vitreous body tissue, membrane from patients with proliferative diabetic retinopathy as experimental group (PDR group), were not associated with other eye disease in patients with idiopathic epiretinal membrane (idiopathic epiretinal membrane, I ERM) as control group (group iERM). The mRNA expression of Wip1 in the anterior membrane was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Immunofluorescence and immunofluorescence were used to detect the protein expression of Wip1 in the anterior membrane. Wip1 and GFAP (the markers of retinal glia) and the co localization of Wip1 and NF- kappa B were observed by double staining with immunofluorescence. Results: a total of 26 cases of retinal anterior membrane were collected, including 17 cases in group PDR and 9 in group iERM. The RT-PCR results showed that the mRNA of Wip1 was highly expressed in the PDR group, but only in the iERM group. The results of immunohistochemistry and immunofluorescence showed that Wip1 had a strong positive signal in the anterior membrane of group PDR. The expression of Wip1, GFAP and NF- kappa B in the anterior membrane of group PDR was correlated by double staining of immunofluorescence. Conclusion: the results of this study suggest that Wip1 may be involved in the formation of fibrous vascular membrane in proliferative diabetic retinopathy through its interaction with NF- kappa B.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R587.2;R774.1

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