本文關(guān)鍵詞：蘆薈苷氧化石墨烯凝膠的制備及其對(duì)創(chuàng)傷修復(fù)作用的研究　出處：《浙江大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 蘆薈苷 氧化石墨烯 水凝膠 創(chuàng)面愈合 疤痕抑制

【摘要】：目的:考察蘆薈苷對(duì)急性創(chuàng)傷的治療效果,進(jìn)一步制備以氧化石墨烯水凝膠為載體的蘆薈苷局部給藥體系,并探討其對(duì)皮膚急性創(chuàng)傷治療和疤痕抑制的作用及其相關(guān)機(jī)制。方法:1.通過(guò)體內(nèi)外一系列實(shí)驗(yàn)考察蘆薈苷對(duì)皮膚創(chuàng)傷的治療效果。體外方面,考察了蘆薈苷對(duì)人永生化角質(zhì)形成細(xì)胞(HaCaT)、人皮膚成纖維細(xì)胞(HSF)以及血管內(nèi)皮細(xì)胞(Endo)的增殖與遷移的影響,采用Tube formation實(shí)驗(yàn)考察蘆薈苷對(duì)血管形成的作用。體內(nèi)實(shí)驗(yàn)方面,通過(guò)建立大鼠急性創(chuàng)傷模型考察蘆薈苷對(duì)傷口愈合率的影響,并對(duì)愈合后的皮膚進(jìn)行掃描電鏡觀察、組織染色(HE、Masson)、免疫組化染色(CD31、EGF)考察愈合后的皮膚微觀結(jié)構(gòu)及組成。2.構(gòu)建攜載蘆薈苷的氧化石墨烯水凝膠,對(duì)處方進(jìn)行優(yōu)化,并進(jìn)一步對(duì)形態(tài)、藥物釋放等進(jìn)行一系列研究。建立凝膠中蘆薈苷的HPLC檢測(cè)方法。3.對(duì)制備的蘆薈苷氧化石墨烯凝膠進(jìn)行體外抑菌作用的考察。在體內(nèi)實(shí)驗(yàn)中,對(duì)蘆薈苷氧化石墨烯凝膠的皮膚刺激性以及創(chuàng)傷修復(fù)實(shí)驗(yàn)做進(jìn)一步的考察。采用掃描電鏡、HE與Masson三色染色、CD31與α-SMA免疫組化染色以及測(cè)定修復(fù)皮膚內(nèi)的VEGF的含量等方法驗(yàn)證蘆薈苷氧化石墨烯凝膠的創(chuàng)傷修復(fù)效果。結(jié)果:1.蘆薈苷對(duì)血管內(nèi)皮細(xì)胞和皮膚成纖維細(xì)胞有一定的促進(jìn)增殖作用,對(duì)HSF細(xì)胞有較強(qiáng)的遷移促進(jìn)作用,同時(shí)還具有一定的抑菌作用,顯示出一定的促進(jìn)愈合的效果。體內(nèi)實(shí)驗(yàn)結(jié)果顯示蘆薈苷溶液能提高傷口愈合率。治療后形成的皮膚,新生血管數(shù)量較多,含有毛囊等附屬器官,膠原蛋白排列較為整齊疏松,與正常皮膚呈現(xiàn)相似的形態(tài)。2.確定制劑處方中各個(gè)成分的含量:蘆薈苷1mg/mL;卡波姆0.5%;氧化石墨烯125μg/mL;三乙醇胺調(diào)節(jié)pH到5。采用本處方制備的蘆薈苷氧化石墨烯凝膠質(zhì)地均勻細(xì)膩,具有合適的粘度,易于涂布,且具有一定的藥物緩釋功能,適合皮膚局部給藥。3.按照處方量配制的蘆薈苷氧化石墨烯凝膠具有良好的抑菌效果,皮膚刺激性實(shí)驗(yàn)證明其沒(méi)有皮膚刺激性。同時(shí),蘆薈苷氧化石墨烯凝膠能提高皮膚創(chuàng)面的傷口愈合率,對(duì)愈合后的皮膚進(jìn)行組織形態(tài)以及免疫組化等考察,結(jié)果表明蘆薈苷氧化石墨烯凝膠能促進(jìn)皮膚血管新生及部分皮膚附屬器官的形成,顯示了一定的疤痕抑制效果。結(jié)論:蘆薈苷氧化石墨烯凝膠可以作為良好的局部給藥體系,促進(jìn)創(chuàng)面的愈合并減少疤痕的形成。
[Abstract]:Objective: To investigate the therapeutic effect of aloin on acute trauma, and further prepare the aloin local drug delivery system based on graphene oxide hydrogel, and to explore its effect on skin acute wound treatment and scar inhibition and its related mechanism. Methods: 1. through a series of experiments in vivo and in vivo, the therapeutic effect of aloin on skin trauma was investigated. In vitro, the effects of aloin on the proliferation and migration of human immortalized keratinocytes (HaCaT), human skin fibroblasts (HSF) and vascular endothelial cells (Endo) were investigated. The effect of aloin on angiogenesis was investigated by Tube formation. In vivo experiment, through the establishment of rat model to investigate the effect of acute trauma of aloin on wound healing rate, and after the healing of skin were observed by scanning electron microscope, tissue staining (HE, Masson), immunohistochemical staining (CD31, EGF) skin microstructure and composition of healing. 2. to construct the graphene oxide hydrogel carrying aloin, optimize the prescription, and further study a series of studies on morphology and drug release. To establish a HPLC method for the determination of aloin in gel. 3. the Bacteriostasis of aloin oxide graphene gel prepared in vitro was investigated. In vivo experiments, the skin irritation of aloin oxide gels and the experiment of trauma repair were further investigated. SEM, HE and Masson trichrome staining, CD31 and -SMA immunohistochemical staining and the content of VEGF in the repaired skin were used to verify the wound healing effect of aloin glucoside graphene gel. Results: 1., aloin has certain promoting effect on proliferation of vascular endothelial cells and dermal fibroblasts, and has a strong migration and promotion effect on HSF cells, and also has a certain bacteriostatic effect, showing a certain effect of promoting healing. The results of the experiment in vivo showed that the aloin solution could improve the healing rate of the wound. After treatment, there are many new skin vessels, including hair follicle and other accessory organs. Collagen is arranged orderly and loose, showing similar morphology with normal skin. 2. to determine the content of each component in the formulation: aloin 1mg/mL; carbomer 0.5%; graphene oxide 125 g/mL triethanolamine; adjust pH to 5. The aloe glucoside graphene oxide gel prepared by this prescription is homogeneous and exquisite in texture. It has the appropriate viscosity and is easy to coat, and has certain drug release function. It is suitable for topical administration of skin. 3. the aloin graphene oxide gel prepared according to the prescription has a good bacteriostasis effect, and the skin irritation test proved that it has no skin irritation. At the same time, aloin graphene oxide gel can improve the healing rate of skin wound wound tissue morphology and immunohistochemical study on the skin after healing, results show that aloin graphene oxide gel can promote the formation of skin angiogenesis and part of skin appendages, showed a certain inhibitory effect of scar. Conclusion: the aloin graphene oxide gel can be used as a good local drug delivery system to promote the healing of the wound and reduce the formation of scar.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R283.6;R285

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