本文關(guān)鍵詞：蘆薈苷氧化石墨烯凝膠的制備及其對創(chuàng)傷修復(fù)作用的研究　出處：《浙江大學》2017年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 蘆薈苷 氧化石墨烯 水凝膠 創(chuàng)面愈合 疤痕抑制

【摘要】：目的:考察蘆薈苷對急性創(chuàng)傷的治療效果,進一步制備以氧化石墨烯水凝膠為載體的蘆薈苷局部給藥體系,并探討其對皮膚急性創(chuàng)傷治療和疤痕抑制的作用及其相關(guān)機制。方法:1.通過體內(nèi)外一系列實驗考察蘆薈苷對皮膚創(chuàng)傷的治療效果。體外方面,考察了蘆薈苷對人永生化角質(zhì)形成細胞(HaCaT)、人皮膚成纖維細胞(HSF)以及血管內(nèi)皮細胞(Endo)的增殖與遷移的影響,采用Tube formation實驗考察蘆薈苷對血管形成的作用。體內(nèi)實驗方面,通過建立大鼠急性創(chuàng)傷模型考察蘆薈苷對傷口愈合率的影響,并對愈合后的皮膚進行掃描電鏡觀察、組織染色(HE、Masson)、免疫組化染色(CD31、EGF)考察愈合后的皮膚微觀結(jié)構(gòu)及組成。2.構(gòu)建攜載蘆薈苷的氧化石墨烯水凝膠,對處方進行優(yōu)化,并進一步對形態(tài)、藥物釋放等進行一系列研究。建立凝膠中蘆薈苷的HPLC檢測方法。3.對制備的蘆薈苷氧化石墨烯凝膠進行體外抑菌作用的考察。在體內(nèi)實驗中,對蘆薈苷氧化石墨烯凝膠的皮膚刺激性以及創(chuàng)傷修復(fù)實驗做進一步的考察。采用掃描電鏡、HE與Masson三色染色、CD31與α-SMA免疫組化染色以及測定修復(fù)皮膚內(nèi)的VEGF的含量等方法驗證蘆薈苷氧化石墨烯凝膠的創(chuàng)傷修復(fù)效果。結(jié)果:1.蘆薈苷對血管內(nèi)皮細胞和皮膚成纖維細胞有一定的促進增殖作用,對HSF細胞有較強的遷移促進作用,同時還具有一定的抑菌作用,顯示出一定的促進愈合的效果。體內(nèi)實驗結(jié)果顯示蘆薈苷溶液能提高傷口愈合率。治療后形成的皮膚,新生血管數(shù)量較多,含有毛囊等附屬器官,膠原蛋白排列較為整齊疏松,與正常皮膚呈現(xiàn)相似的形態(tài)。2.確定制劑處方中各個成分的含量:蘆薈苷1mg/mL;卡波姆0.5%;氧化石墨烯125μg/mL;三乙醇胺調(diào)節(jié)pH到5。采用本處方制備的蘆薈苷氧化石墨烯凝膠質(zhì)地均勻細膩,具有合適的粘度,易于涂布,且具有一定的藥物緩釋功能,適合皮膚局部給藥。3.按照處方量配制的蘆薈苷氧化石墨烯凝膠具有良好的抑菌效果,皮膚刺激性實驗證明其沒有皮膚刺激性。同時,蘆薈苷氧化石墨烯凝膠能提高皮膚創(chuàng)面的傷口愈合率,對愈合后的皮膚進行組織形態(tài)以及免疫組化等考察,結(jié)果表明蘆薈苷氧化石墨烯凝膠能促進皮膚血管新生及部分皮膚附屬器官的形成,顯示了一定的疤痕抑制效果。結(jié)論:蘆薈苷氧化石墨烯凝膠可以作為良好的局部給藥體系,促進創(chuàng)面的愈合并減少疤痕的形成。
[Abstract]:Objective: To investigate the therapeutic effect of aloin on acute trauma, and further prepare the aloin local drug delivery system based on graphene oxide hydrogel, and to explore its effect on skin acute wound treatment and scar inhibition and its related mechanism. Methods: 1. through a series of experiments in vivo and in vivo, the therapeutic effect of aloin on skin trauma was investigated. In vitro, the effects of aloin on the proliferation and migration of human immortalized keratinocytes (HaCaT), human skin fibroblasts (HSF) and vascular endothelial cells (Endo) were investigated. The effect of aloin on angiogenesis was investigated by Tube formation. In vivo experiment, through the establishment of rat model to investigate the effect of acute trauma of aloin on wound healing rate, and after the healing of skin were observed by scanning electron microscope, tissue staining (HE, Masson), immunohistochemical staining (CD31, EGF) skin microstructure and composition of healing. 2. to construct the graphene oxide hydrogel carrying aloin, optimize the prescription, and further study a series of studies on morphology and drug release. To establish a HPLC method for the determination of aloin in gel. 3. the Bacteriostasis of aloin oxide graphene gel prepared in vitro was investigated. In vivo experiments, the skin irritation of aloin oxide gels and the experiment of trauma repair were further investigated. SEM, HE and Masson trichrome staining, CD31 and -SMA immunohistochemical staining and the content of VEGF in the repaired skin were used to verify the wound healing effect of aloin glucoside graphene gel. Results: 1., aloin has certain promoting effect on proliferation of vascular endothelial cells and dermal fibroblasts, and has a strong migration and promotion effect on HSF cells, and also has a certain bacteriostatic effect, showing a certain effect of promoting healing. The results of the experiment in vivo showed that the aloin solution could improve the healing rate of the wound. After treatment, there are many new skin vessels, including hair follicle and other accessory organs. Collagen is arranged orderly and loose, showing similar morphology with normal skin. 2. to determine the content of each component in the formulation: aloin 1mg/mL; carbomer 0.5%; graphene oxide 125 g/mL triethanolamine; adjust pH to 5. The aloe glucoside graphene oxide gel prepared by this prescription is homogeneous and exquisite in texture. It has the appropriate viscosity and is easy to coat, and has certain drug release function. It is suitable for topical administration of skin. 3. the aloin graphene oxide gel prepared according to the prescription has a good bacteriostasis effect, and the skin irritation test proved that it has no skin irritation. At the same time, aloin graphene oxide gel can improve the healing rate of skin wound wound tissue morphology and immunohistochemical study on the skin after healing, results show that aloin graphene oxide gel can promote the formation of skin angiogenesis and part of skin appendages, showed a certain inhibitory effect of scar. Conclusion: the aloin graphene oxide gel can be used as a good local drug delivery system to promote the healing of the wound and reduce the formation of scar.
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R283.6;R285

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