本文關(guān)鍵詞：同步輻射原位微流設(shè)備的研制及在蛋白質(zhì)研究中的應(yīng)用　出處：《中國科學(xué)院研究生院(上海應(yīng)用物理研究所)》2016年博士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 同步輻射 小角X射線散射 原位微流設(shè)備 蛋白質(zhì)折疊

【摘要】：同步輻射小角X射線散射技術(shù)是解析物質(zhì)結(jié)構(gòu)信息的一種技術(shù)手段,因其特有優(yōu)勢,被廣泛應(yīng)用于凝聚態(tài)物理、生物化學(xué)、材料科學(xué)等學(xué)科領(lǐng)域。其中,蛋白質(zhì)等生物大分子溶液就是同步輻射小角散射技術(shù)研究的重要體系之一。利用同步輻射小角X射線散射技術(shù),可對(duì)溶液狀態(tài)(更接近生理狀態(tài))下的生物大分子樣品進(jìn)行微觀結(jié)構(gòu)和形態(tài)的研究。為了進(jìn)一步提升同步輻射小角X射線散射實(shí)驗(yàn)線站針對(duì)溶液樣品的原位實(shí)驗(yàn)?zāi)芰?拓展其在蛋白質(zhì)等生物大分子結(jié)構(gòu)研究中的應(yīng)用,本論文主要的工作是結(jié)合具體的實(shí)驗(yàn)需求,研制可用于同步輻射小角X射線散射實(shí)驗(yàn)的原位微流設(shè)備,并采用研制的原位微流設(shè)備進(jìn)行了蛋白質(zhì)的微觀結(jié)構(gòu)變化的應(yīng)用研究。主要的工作內(nèi)容如下:1.設(shè)計(jì)并建立了一種可控微流體蠕動(dòng)設(shè)備。采用LabVIEW軟件編寫了此蠕動(dòng)設(shè)備的控制程序,通過實(shí)驗(yàn)對(duì)可控微流體蠕動(dòng)設(shè)備的進(jìn)樣量、溶液粘度與蠕動(dòng)速度的關(guān)系進(jìn)行了標(biāo)定,并將此設(shè)備安裝于同步輻射小角X射線散射實(shí)驗(yàn)線站,使用溶菌酶蛋白質(zhì)溶液樣品,對(duì)該設(shè)備的防輻射損傷性能進(jìn)行了實(shí)驗(yàn)驗(yàn)證。此外,還對(duì)該設(shè)備進(jìn)行了硬件及軟件的優(yōu)化升級(jí)。2.設(shè)計(jì)并建立了一種溫度跳變微流芯片原位研究設(shè)備。利用ANSYS CFX流體力學(xué)軟件對(duì)多種微流通道結(jié)構(gòu)的流體換熱特性進(jìn)行了有限元分析,根據(jù)數(shù)值分析結(jié)果,確定了微流芯片的加熱方案,完成了微流芯片通道結(jié)構(gòu)的物理設(shè)計(jì),并提出了實(shí)際加工方案。通過熒光染料溫度指示劑標(biāo)定方法對(duì)微流芯片的跳變溫度進(jìn)行了標(biāo)定,并根據(jù)實(shí)驗(yàn)結(jié)果對(duì)微流芯片原型設(shè)計(jì)進(jìn)行了優(yōu)化改進(jìn)。最后,將微流設(shè)備安裝于同步輻射小角X射線散射實(shí)驗(yàn)線站,進(jìn)行了蛋白質(zhì)的復(fù)折疊過程的時(shí)間分辨SAXS研究。本論文的工作內(nèi)容以實(shí)際實(shí)驗(yàn)需求為起點(diǎn),研制了兩種用于同步輻射小角X射線散射實(shí)驗(yàn)的原位微流設(shè)備,推動(dòng)了上海光源同步輻射實(shí)驗(yàn)技術(shù)在溶液樣品中的應(yīng)用,對(duì)提升同步輻射溶液樣品原位小角散射實(shí)驗(yàn)?zāi)芰哂兄匾囊饬x。
[Abstract]:Synchrotron small angle X ray scattering technique is a kind of technical means of analytical information of material structure, because of its unique advantages, is widely used in condensed matter physics, biochemistry, materials science and other fields. Among them, one of the important system of bio macromolecules such as protein solution is on synchrotron radiation scattering technique using synchrotron small angle. Radiation small angle X ray scattering technique, the solution state (closer to the physiological state of biological macromolecules) to study the sample microstructure and morphology. In order to further enhance the synchrotron small angle X ray scattering experimental station for in situ experiments of solution samples, expand its application in the research of protein structure of biological macromolecules in this paper, the main work is the combination of the specific requirements of the development of experiment, can be used for in situ synchrotron small angle X ray scattering experiment of micro flow equipment, and using research Application of in situ micro flow equipment for the microstructure change of the protein. The main contents are as follows: 1. the design and establishment of a controllable micro peristaltic fluid equipment. The control program is compiled by using LabVIEW software the peristaltic equipment, the amount of sample experiment of controllable micro peristaltic fluid equipment, the relationship between viscosity and creep the speed was calibrated, and the equipment installed in the synchrotron small angle X ray scattering experimental station, using lysozyme protein solution samples of radiation damage performance of the device was tested. In addition, the device is optimized for hardware and software upgrade.2. designed and built a temperature jump in situ study of microfluidic chip devices. Fluid microchannel structure of using ANSYS CFX CFD software the heat transfer characteristics of the finite element analysis, according to the results of numerical analysis,. The heating program set a microfluidic chip, completed the physical design of microfluidic chip channel structure, and put forward the actual processing scheme. Through the fluorescent dye temperature indicator calibration method to calibrate the temperature jump of microfluidic chips, and according to the experimental results of the prototype design of microfluidic chip is improved. Finally, the micro flow equipment installed at the synchrotron small angle X ray scattering experimental station, the complex folding of the time-resolved SAXS study. The content of the thesis to the actual experimental demand as the starting point, two for in situ synchrotron small angle X ray scattering experiment of micro flow equipment was developed and promoted the application of Shanghai the light source synchrotron radiation technology in solution, has an important significance for enhancing the ability of small angle X-ray scattering of synchrotron radiation solution samples in situ.

【學(xué)位授予單位】：中國科學(xué)院研究生院(上海應(yīng)用物理研究所)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：Q51;O434.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 洪春霞;周平;李怡雯;曾建榮;邊風(fēng)剛;王R，

本文編號(hào)：1414550