天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

當(dāng)前位置:主頁(yè) > 碩博論文 > 工程博士論文 >

兩種提取工藝對(duì)金福菇和羊肚菌多糖的影響

發(fā)布時(shí)間:2024-12-21 01:11
  食用菌多糖具有諸多藥理作用,是食用菌中重要的生物活性成分。眾所周知的,提取方法會(huì)極大的影響食用菌多糖的化學(xué)結(jié)構(gòu),進(jìn)而影響其生物活性,采用新型快速、有效的多糖提取技術(shù)對(duì)食用菌多糖具有重要意義。在本文中,我們研究了兩種新提取技術(shù)對(duì)金福菇(Tricholoma lobayense)和羊肚菌(Morchella esculenta)多糖的提取效果,闡明了提取方法對(duì)其提取率、結(jié)構(gòu)及生物活性的影響。主要研究?jī)?nèi)容如下:1.閃提對(duì)金福菇多糖結(jié)構(gòu)及抗氧化性的影響以水為溶劑對(duì)金福菇多糖進(jìn)行閃式提取,對(duì)獲得的多糖TLH-G進(jìn)行結(jié)構(gòu)解析和抗氧化性研究。研究發(fā)現(xiàn)TLH-G是一種分子量為4.1×10~3 Da的雜多糖,總糖、蛋白質(zhì)和糖醛酸的含量分別為99.10%、0.72%和13.85%。單糖組成為甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖和半乳糖,摩爾比為1.00:0.33:0.56:14.13:2.92,其主鏈由→1)-β-D-Glcp-(6→和→1)-α-D-Galp-(3→構(gòu)成?寡趸匝芯勘砻鱐LH-G的DPPH和ABTS自由基的清除的IC50值分別為0.43 mg/mL和>0.5 m...

【文章頁(yè)數(shù)】:102 頁(yè)

【學(xué)位級(jí)別】:博士

【文章目錄】:
Acknowledgements
摘要
abstract
Chapter1.Introduction to polysaccharides from edible fungi and their extraction techniques
    1.1.Medicinal mushrooms
    1.2.Nutritious significance of mushrooms
    1.3.Mushroom bioactives
        1.3.1.Mushroom polysaccharides
            1.3.1.1.Glucans
            1.3.1.2.Chitin and chitosan
            1.3.1.3.Mannans and mannoproteins
        1.3.2.Other nutritious portions of mushrooms
    1.4.Structural properties of mushroom polysaccharides
    1.5.Conventional extraction methods for mushrooms
        1.5.1.Solvent-assisted extraction(SAE)
            1.5.1.1.Hydro-alcohol extractions
    1.6.Emerging technologies for polysaccharide extraction
        1.6.1.Ultrasound-assisted extraction(UAE)
        1.6.2.Enzyme-assisted extraction(EAE)
        1.6.3.Microwave-assisted extraction(MAE)
        1.6.4.Accelerated-solvent Extraction(ASE)or pressurized liquid extraction(PLE)
        1.6.5.Supercritical fluid extraction(SFE)
        1.6.6.Pulsed-electric field extraction(PEF)
        1.6.7.High-pressure homogenization extraction(HPH)
        1.6.8.Flash-assisted extraction(FAE)
        1.6.9.Hydrothermal extraction or subcritical water/pressurized hot water extraction
            1.6.9.1.Mechanisms involved during hydrothermal extraction
            1.6.9.2.Parameters affecting hydrothermal extraction
    Aims and objectives
    References
Chapter2.Structural elucidation and antioxidant activity of a novel heteroglycan from Tricholoma lobayense by flash extraction
    2.1.Introduction
    2.2.Experimental Section
        2.2.1.Materials and apparatus
        2.2.2.Extraction procedure
        2.2.3.Deproteination and purification
        2.2.4.Physical and chemical properties analysis
        2.2.5.Analysis of monosaccharide composition
        2.2.6.Methylation and GC-MS analysis
        2.2.7.NMR spectroscopy
        2.2.8.Antioxidant activity assays
            2.2.8.1.DPPH radical scavenging assay
            2.2.8.2.ABTS assay
    2.3.Results and discussions
        2.3.1.Physical and chemical properties of TLH-G
        2.3.2.FT-IR analysis of TLH-G
        2.3.3.TLH-G monosaccharide analysis and their effect on antioxidant activity
        2.3.4.Structural characterization of TLH-G via GC-MS analysis
        2.3.5 NMR analysis of TLH-G
        2.3.6.Antioxidant activity evaluation by DPPH and ABTS assays
        2.3.7.Glycosidic bond types in TLH-G polysaccharides
    References
Chapter3.Extraction of polysaccharides from Tricholoma lobayense by hydrothermal methodology
    3.1.Introduction
    3.2.Experimental section
        3.2.1.Materials and methods
        3.2.2.Extraction of Tricholoma lobayense mushroom
        3.2.3.Total carbohydrate and protein content
        3.2.4.Extraction at ideal conditions
        3.2.5.Purification of T.lobayense polysaccharides
        3.2.6.Antioxidant activity assays
            3.2.6.1.DPPH radical scavenging activity assay
            3.2.6.2.ABTS radical scavenging activity assay
            3.2.6.3.Hydroxyl radical scavenging activity assay
            3.2.6.4.Reducing power
            3.2.6.5.Superoxide radical scavenging activity assay
    3.3.Results and discussions
        3.3.1.Extraction parameters and optimization
        3.3.2.Optimization of ratio of solid to liquid(g/mL)
        3.3.3.Optimization of temperature
        3.3.4.Optimization of extraction time
        3.3.5.In vitro antioxidant assays
    References
Chapter4.Effects of hydrothermal extraction on polysaccharides from Morchella esculenta
    4.1 Introduction
    4.2.Experimental Section
        4.2.1.Materials and reagents
        4.2.2.Extraction of mushroom
        4.2.3.Purification of Morchella esculenta polysaccharides
        4.2.4.Total carbohydrate and protein content analysis
        4.2.5.In vitro Antioxidant activity assays
            4.2.5.1.DPPH radical scavenging assay
            4.2.5.2.ABTS radical scavenging assay
            4.2.5.3.Reducing power
            4.2.5.4.Hydroxyl radical scavenging assay
        4.2.6.Ethanol gradient precipitation
        4.2.7.HPLC analysis
        4.2.8.CTAB treatment
    4.3.Results and discussions
        4.3.1.Total sugar,protein,and in vitro antioxidant activities of M.esculenta polysaccharides
        4.3.2.Fractionation by ethanol gradient precipitation and their HPLC analysis
    References
Chapter5.Conclusions
Publications
Author’s profile



本文編號(hào):4018154

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/shoufeilunwen/gckjbs/4018154.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶da900***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
久久精品国产亚洲av麻豆尤物| 一区二区三区日韩中文| 不卡一区二区在线视频| 国产欧美日产久久婷婷| 尤物天堂av一区二区| 中文字幕欧美精品人妻一区| 亚洲欧洲日韩综合二区| 色涩一区二区三区四区| 日韩一本不卡在线观看| 精品伊人久久大香线蕉综合| 国产精品免费视频久久| 粉嫩一区二区三区粉嫩视频| 老司机精品国产在线视频| 视频一区日韩经典中文字幕| 伊人天堂午夜精品草草网| 五月婷婷综合缴情六月| 国产欧美日韩精品一区二| 精品国产亚洲免费91| 欧美成人高清在线播放| 熟女一区二区三区国产| 欧美大胆女人的大胆人体| 成年人免费看国产视频| 国产成人精品久久二区二区| 一区二区三区亚洲天堂| 亚洲淫片一区二区三区| 国内九一激情白浆发布| 日韩日韩日韩日韩在线| 中文字幕日韩无套内射| 情一色一区二区三区四| 国产成人精品一区二三区在线观看| 日韩欧美三级视频在线| 成人国产一区二区三区精品麻豆 | 经典欧美熟女激情综合网| 亚洲中文字幕高清视频在线观看| 日本一区二区三区久久娇喘| 色婷婷成人精品综合一区| 国产午夜精品美女露脸视频| 久久精品国产亚洲av麻豆尤物| 国产精品免费福利在线| 亚洲一区二区三区四区性色av| 国产一区二区三区丝袜不卡 |