本文關(guān)鍵詞：SSRF生物大分子光束線站自動化集成及實驗技術(shù)發(fā)展　出處：《中國科學(xué)院研究生院(上海應(yīng)用物理研究所)》2017年博士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 生物大分子晶體學(xué)光束線站 控制與數(shù)據(jù)采集 微晶體衍射 微束準直器 復(fù)合折射透鏡

【摘要】：上海光源生物大分子晶體學(xué)光束線站BL17U1是我國第三代同步輻射裝置上的首條高性能光束線,是通過晶體學(xué)方法開展生物大分子三維結(jié)構(gòu)研究的重要平臺。三代同步輻射裝置提供了高亮度高穩(wěn)定性的光源,為了充分利用光束線的光學(xué)以及硬件設(shè)備性能,方便用戶群體高效地開展晶體學(xué)衍射數(shù)據(jù)采集,高度集成的自動化數(shù)據(jù)采集系統(tǒng)是光束線技術(shù)的重要組成部分。得益于同步輻射領(lǐng)域的開放與合作,上海光源在建設(shè)過程中,采用了EPICS開發(fā)環(huán)境�；贓PICS環(huán)境開發(fā)的運動控制和數(shù)據(jù)獲取可以滿足光束線調(diào)試及運行的需求。實驗站設(shè)備底層控制同樣采用EPICS開發(fā)環(huán)境,為了解決衍射實驗中的曝光時間的準確性,部分功能由硬件層的代碼實現(xiàn)。通過調(diào)研國際上同類的實驗站用戶控制及數(shù)據(jù)采集系統(tǒng),發(fā)現(xiàn)生物大分子晶體學(xué)線站因為其自身特點,需要提供給用戶界面直觀、易于操作、功能完善的數(shù)據(jù)采集系統(tǒng)。斯坦福開發(fā)的Blu-Ice/DCS是最早實現(xiàn)該類功能的使用于生物大分子晶體學(xué)光束線站的系統(tǒng),并被多條光束線借鑒。在本論文的工作中,通過EPICS應(yīng)用及相關(guān)硬件服務(wù)器開發(fā),我完成了基于開源系統(tǒng)的線站控制與數(shù)據(jù)采集自動化系統(tǒng)的開發(fā)工作。根據(jù)光束線的實際硬件特性及控制結(jié)構(gòu),開發(fā)的各項功能齊全、圖形化界面方便用戶實驗,例如能量自動聯(lián)動、自動化熒光掃描、集成自動化機械手等。該自動化集成系統(tǒng),成功地應(yīng)用于上海光源首條生物大分子晶體學(xué)光束線站,為該光束線的運行開放提供了充分的技術(shù)保障,也為今后的新功能開發(fā)提供了良好的基礎(chǔ)。結(jié)合晶體學(xué)實驗技術(shù)的發(fā)展,微晶體衍射在結(jié)構(gòu)解析方面發(fā)揮著越來越重要的作用,而微細光束是開展微晶體衍射的前提。為了獲得微細光束,在已經(jīng)運行的生物大分子晶體學(xué)光束線上,微束準直器的發(fā)展可以快速地獲得不同尺寸的束斑。同類儀器成功地應(yīng)用于國際上其它線站,并通過實驗數(shù)據(jù)采集與對比,證明了微細光束的重要性。為了在上海光源現(xiàn)有生物大分子光束線上實現(xiàn)該功能,結(jié)合衍射儀的硬件布局,我發(fā)展了適用于光束線BL17U1的微細準直器儀器,該準直器兼容于現(xiàn)有衍射儀系統(tǒng)。對獲得的微細束斑特性進行了測量,并應(yīng)用于數(shù)據(jù)采集。對于微晶體衍射,因其衍射能力一般比大尺寸的晶體弱,在束斑相同的情況下,提供更多的光子對結(jié)構(gòu)解析有重要幫助。為了提供微細光束的光通量,我采用復(fù)合折射透鏡聚焦方案,設(shè)計了利用復(fù)合折射透鏡聚焦獲得微細光束的模式,獲得了光通量密度增益并開展相關(guān)數(shù)據(jù)采集的研究,為在該線站開展微晶體衍射實驗提供了充分技術(shù)準備。
[Abstract]:Shanghai light source biological macromolecule crystallographic beamline station BL17U1 is the first high-performance beamline on the third generation synchrotron radiation facility in China. It is an important platform for studying the three-dimensional structure of biological macromolecules by crystallography. The three generation synchrotron radiation device provides high brightness and high stability of the light source, in order to make full use of the optical beam line and the hardware performance, convenient user groups to effectively carry out the crystallographic diffraction data, highly integrated automation data acquisition system is an important part of the beam line technology. Thanks to the openness and cooperation in the field of synchrotron radiation, the Shanghai light source has adopted the EPICS development environment in the process of construction. The motion control and data acquisition based on the EPICS environment can meet the needs of the beam line debugging and operation. The bottom of the experimental station is also controlled by the EPICS development environment. In order to solve the accuracy of the exposure time in the diffraction experiment, some functions are implemented by the code of the hardware layer. Through investigating the international similar experimental station user control and data acquisition system, it is found that the macromolecular crystallographic line station, because of its own characteristics, needs to provide the user interface with intuitive, easy operation and perfect data acquisition system. The Blu-Ice/DCS developed by Standford is the first system to use this kind of function in the beam line station of the macromolecular crystallography, and is used for reference by a number of beam lines. In the work of this thesis, through EPICS application and related hardware server development, I completed the development of line station control and data acquisition automation system based on open source system. According to the actual hardware characteristics and control structure of the beamline, all functions developed, graphical interfaces are convenient for user experiments, such as energy automatic linkage, automatic fluorescence scanning, integrated automation manipulator and so on. The automatic integrated system has been successfully applied to the first biological macromolecular crystallographic beamline station of Shanghai light source, providing sufficient technical support for the operation and opening of the beamline, and also providing a good foundation for the development of new functions in the future. Combined with the development of crystallographic experimental technology, microcrystal diffraction plays a more and more important role in structural analysis. Micro beam is the prerequisite for developing microcrystal diffraction. In order to obtain the micro beam, the development of the microbeam collimator can quickly obtain the beam spots of different sizes on the already operating biological macromolecule crystallographic beam line. Similar instruments have been successfully applied to other international line stations, and the importance of micro beam is proved by the acquisition and comparison of experimental data. In order to realize this function on the existing biologic macromolecule beam line in Shanghai light source, combined with the hardware layout of the diffractometer, I developed a micro collimator instrument suitable for beam line BL17U1, which is compatible with the existing diffractive instrument system. The characteristics of the obtained micro beam spot are measured and applied to data acquisition. For microcrystalline diffraction, its diffraction ability is generally weaker than that of large size crystal. When the beam spot is the same, providing more photons is helpful for structural analysis. In order to provide micro beam flux, I adopt the compound refractive lens focusing scheme is designed by using compound refractive lens focusing obtain fine beam mode, the flux density gain and carry out research related to data collection, in the line station to carry out micro crystal diffraction experiment provides sufficient technical preparation.
【學(xué)位授予單位】：中國科學(xué)院研究生院(上海應(yīng)用物理研究所)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2017
【分類號】：O734

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