【摘要】：隨著我國經(jīng)濟(jì)的快速發(fā)展,我國人民的生活水平得到了飛速的提升。與過去相比,我國人民飲食喜歡和飲食結(jié)構(gòu)發(fā)生了巨大的變化,肉制品成為飯桌上的常客,并且隨著生活節(jié)奏的加快,外食成了目前人們的主要就餐方式。這一系列的改變都導(dǎo)致人民飲食攝取的油脂大幅度增加,高血脂、心腦血管疾病的發(fā)生率也在大大的增加。血脂異常是導(dǎo)致這一系列疾病的重要因素,它是指人體內(nèi)甘油三酯、總膽固醇、高密度脂蛋白和低密度脂蛋白等一系列指標(biāo)異常。絞股藍(lán)(Gynostemma pentaphyllum Thunb.Makion)具有改善脂質(zhì)代謝、抗癌防衰、滋補(bǔ)保健、增強(qiáng)體質(zhì)等多種功能,有著"南方人參"之美譽(yù)。絞股藍(lán)皂苷是它的主要活性物質(zhì),具有降血糖、降血脂、保護(hù)心腦血管、抗腫瘤、抗氧化及抗炎等生理活性,越來越受到國內(nèi)外學(xué)者的關(guān)注。本課題以降血脂有效成分絞股藍(lán)達(dá)瑪烷型四環(huán)三萜類化合物為研究對象,通過建立體外HepG2肝細(xì)胞脂肪堆積模型,評價(jià)絞股藍(lán)皂苷清除肝細(xì)胞脂肪堆積的能力。主要的研究內(nèi)容和結(jié)論如下:采用MTT法,篩選了 5個絞股藍(lán)皂苷對HepG2細(xì)胞活力的影響,確定化合物SL-1和SL-2為合適的樣品,并且明確合適的給藥濃度范圍為10μg/ml-100 μg/ml。通過反復(fù)柱層析法,從絞股藍(lán)總皂苷中快速分離制備絞股藍(lán)皂苷SL-1和SL-2。利用油酸誘導(dǎo)建立HepG2細(xì)胞脂肪堆積模型,從細(xì)胞內(nèi)脂滴形態(tài)、總膽固醇(TC值)、甘油三酯(TG值)、高密度脂蛋白膽固醇(HDL-c值)、低密度脂蛋白膽固醇(LDL-c值)等方面評價(jià)絞股藍(lán)皂苷的降脂作用。試驗(yàn)結(jié)果表明,絞股藍(lán)皂苷SL-1和SL-2均可明顯減輕細(xì)胞內(nèi)脂質(zhì)堆積現(xiàn)象,顯著降低細(xì)胞中TG、TC含量,其中100μg/ml絞股藍(lán)皂苷SL-1對TG、TC的清除率分別為達(dá)54.8%和52.7%;100 μg/ml絞股藍(lán)皂苷SL-2對TG、TC的清除率分別為達(dá)78.3%和80.2%;并且高濃度的SL-1和SL-2對HDL和LDL也有很好的影響作用。由此可見,兩種絞股藍(lán)皂苷均具有良好的體外降脂活性,對脂肪肝有較好的預(yù)防效果。
[Abstract]:With the rapid development of our economy, our people's living standards have been rapidly improved. Compared with the past, the Chinese people's diet and diet structure have undergone great changes, meat products have become frequent guests on the table, and with the acceleration of life rhythm, external food has become the main way for people to eat at present. This series of changes have led to a significant increase in people's dietary fat intake, hyperlipidemia, cardiovascular and cerebrovascular diseases. Dyslipidemia is an important factor leading to this series of diseases. It refers to a series of abnormal indexes such as triglyceride, total cholesterol, high density lipoprotein and low density lipoprotein. Gynostemma pentaphyllum (Gynostemma pentaphyllum Thunb.Makion) has many functions, such as improving lipid metabolism, anticancer and anti-aging, nourishing health care, strengthening physique and so on. Gynostemma pentaphyllum saponins are the main active substances, which have the physiological activities of lowering blood sugar, lowering blood lipids, protecting heart and cerebrovascular vessels, anti-tumor, anti-oxidation and anti-inflammation and so on, which are paid more and more attention by scholars at home and abroad. The aim of this study was to evaluate the ability of gynostemma pentaphyllum saponins (Gypenosides) to scavenge lipid accumulation in hepatocytes by establishing an in vitro HepG2 hepatocyte fat accumulation model. The main contents and conclusions were as follows: by MTT method, the effects of five gynostemosides on the viability of HepG2 cells were screened, and the suitable samples of SL-1 and SL-2 were determined, and the suitable concentration range was 10 渭 g/ml-100 渭 g / ml. Rapid separation and preparation of SL-1 and SL-2. from total saponins of gynostemma pentaphyllum by repeated column chromatography The lipid accumulation model of HepG2 cells was established by oleic acid induction. Total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-c) and low density lipoprotein cholesterol (LDL-c) were used to evaluate the antilipidemic effect of gynostemma pentaphyllum saponins. The results showed that both ginsenoside SL-1 and SL-2 could significantly reduce the accumulation of lipid and the content of TG,TC in the cells. The clearance rates of 100 渭 g/ml gynosaponin SL-1 to TG,TC were 54.8% and 52.7 渭 g/ml, respectively, and the clearance rates of SL-2 to TG,TC were 78.3% and 80.2%, respectively, and the high concentrations of SL-1 and SL-2 also had a good effect on HDL and LDL. It can be seen that both gynostemma pentaphyllum saponins have good lipid-lowering activity in vitro and have a better preventive effect on fatty liver.
【學(xué)位授予單位】：沈陽農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TS201.4
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本文編號：2221996