【摘要】：“玉金香”甜瓜(Cucumis melo L.cv.Yujinxiang)是呼吸躍變型果實(shí),BTH處理可誘導(dǎo)其抗病性,影響果實(shí)香氣物質(zhì)等次生代謝。磷脂是果實(shí)細(xì)胞膜的主要構(gòu)成組分,磷脂酶作用下可水解為脂肪酸、甘油、磷酸和氨基醇等,進(jìn)而參與香氣等次生代謝。采后BTH處理“玉金香”甜瓜果實(shí),貯藏期間監(jiān)測PLA2、PLC和PLD活性及其基因表達(dá)特性,分析BTH誘抗處理對(duì)磷脂代謝底物、產(chǎn)物以及代謝酶活的影響,確定磷脂代謝與果實(shí)香氣釋放的相關(guān)性,探討采后BTH誘抗處理對(duì)甜瓜果實(shí)細(xì)胞膜磷脂代謝和香氣釋放的影響機(jī)理。結(jié)果顯示:1、采后BTH處理可抑制磷脂組分PC、PI降解,降低磷脂代謝產(chǎn)物PA和脂肪酸的含量,但在貯藏后期BTH處理組脂肪酸不飽和度高于對(duì)照。2、采后BTH處理降低PLA2、PLC和PLD的基因相對(duì)表達(dá)量和酶活性。3、采后BTH處理減少了樣品亞油酸代謝的C6、C9香氣組分釋放量,抑制了亞麻酸代謝的C6、C9醇醛類和油酸代謝的含有丁基、戊基的香氣組分及其大部分衍生酯類的釋放,促進(jìn)了乙酸(Z)-3-己烯酯、乙酸3,6-壬二烯酯、乙酸異丁酯酯類物質(zhì)的釋放。4、果實(shí)脂肪酸代謝香氣物質(zhì)總量與乙烯釋放的相關(guān)性系數(shù)較磷脂代謝大,呈極顯著正相關(guān)(P0.01),與PLA2活性呈顯著正相關(guān)(P0.05)、與PI呈顯著負(fù)相關(guān)(P0.05),其它因素與其相關(guān)性不顯著。乙烯釋放與脂肪酸代謝香氣物質(zhì)總量的相關(guān)性高于乙烯釋放與磷脂代謝,且高于磷脂代謝與脂肪酸代謝香氣總量的相關(guān)性。PLA2、磷脂代謝產(chǎn)物不飽和脂肪酸與香氣組分釋放相關(guān)性分別呈顯著正相關(guān)和負(fù)相關(guān)(P0.05)。BTH處理后削弱乙烯釋放、磷脂代謝與香氣物質(zhì)之間的相關(guān)性。綜上所述,采后BTH處理可通過抑制磷脂組分降解、磷脂酶活性及其基因相對(duì)表達(dá)量減少產(chǎn)物磷脂酸和不飽和脂肪酸的生成,進(jìn)而抑制不飽和脂肪酸代謝的香氣物質(zhì)的釋放;由乙烯釋放與脂肪酸代謝香氣組分釋放的相關(guān)性高于磷脂代謝,可推斷脂肪酸代謝香氣組分的前體物質(zhì)代謝—磷脂代謝對(duì)脂肪酸代謝香氣組分釋放的影響度可能小于后期的LOX代謝,乙烯是影響脂肪酸代謝香氣組分的關(guān)鍵因素。采后BTH處理降低了乙烯釋放、磷脂代謝與香氣物質(zhì)的相關(guān)性,削弱了乙烯釋放與磷脂代謝對(duì)香氣物質(zhì)釋放的影響。
[Abstract]:"Yujinxiang" muskmelon (Cucumis melo L.cv.Yujinxiang is a kind of fruit with respiratory jump, which can induce its disease resistance and affect the secondary metabolism of aroma substances. Phospholipid is the main component of fruit cell membrane. Phospholipase can be hydrolyzed into fatty acid, glycerol, phosphoric acid and amino alcohol, and then participate in the secondary metabolism of aroma. Postharvest BTH was used to treat muskmelon fruits. During storage, the activities of PLA2,PLC and PLD and their gene expression were monitored, and the effects of BTH induced resistance treatment on the activities of phospholipid metabolites, metabolites and enzymes were analyzed. To determine the relationship between phospholipid metabolism and aroma release, and to explore the mechanism of the effect of postharvest BTH induced treatment on lipid metabolism and aroma release of muskmelon fruit cell membrane. The results showed that BTH treatment after harvest could inhibit the degradation of phospholipid component PC,PI and decrease the content of PA and fatty acid, the metabolite of phospholipid. However, the unsaturation of fatty acids in the BTH treatment group was higher than that in the control group at the late stage of storage. The relative expression and enzyme activity of PLA2,PLC and PLD were decreased in postharvest BTH treatment, and the release of C6C9 aroma components of linoleic acid metabolism in the samples was decreased by postharvest BTH treatment. The aroma components of Ding Ji, pentyl group and most of their derivative esters were inhibited by the metabolism of linolenic acid, such as C _ 6C _ 9 alcoholaldehyde and oleic acid metabolism, which promoted the release of (Z) -3-hexene acetate and 3nylenediene acetate. The correlation coefficient between the total amount of fatty acid metabolic aroma and ethylene release was higher than that of phospholipid metabolism. There was a significant positive correlation (P0.01), a significant positive correlation with PLA2 activity (P0.05), a significant negative correlation with PI (P0.05), and no significant correlation between other factors. The correlation between ethylene release and fatty acid metabolic total aroma substance was higher than that of ethylene release and phospholipid metabolism. And higher than that of phospholipid metabolism and total aroma of fatty acid metabolism. PLA2. The relationship between unsaturated fatty acid and aroma component release was significantly positive and negative correlation (P0.05). BTH treatment weakened ethylene release. The correlation between phospholipid metabolism and aroma substances. In conclusion, BTH treatment after harvest can inhibit the degradation of phospholipid components, reduce the production of phosphatidic acid and unsaturated fatty acids by phospholipase activity and the relative expression of their genes, and then inhibit the release of aroma substances from unsaturated fatty acid metabolism. The correlation between ethylene release and aroma component release of fatty acid metabolism was higher than that of phospholipid metabolism. It can be concluded that the effect of metabolism of phospholipid on the release of aroma components of fatty acid metabolism may be less than that of LOX in the later stage. Ethylene is the key factor affecting the aroma component of fatty acid metabolism. Postharvest BTH treatment decreased ethylene release, the correlation between phospholipid metabolism and aroma substances, and the effect of ethylene release and phospholipid metabolism on aroma release.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TS255.3

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本文編號(hào)：2205614