本文選題：熒光探針 + 芘��； 參考：《鄭州大學(xué)》2017年碩士論文

【摘要】：熒光探針因其操作簡便、靈敏度高、響應(yīng)快速、成本低廉、不損樣品等眾多優(yōu)點而備受人們關(guān)注,并被廣泛的應(yīng)用在化學(xué)、生物學(xué)和醫(yī)學(xué)等研究領(lǐng)域。芘及其衍生物是一類性能優(yōu)異的熒光材料,具有化學(xué)結(jié)構(gòu)穩(wěn)定、易于合成、量子產(chǎn)率高、熒光壽命長等特點,尤其是在特定的條件下芘環(huán)可以發(fā)生單體與激基締合物之間的相互轉(zhuǎn)換,為其在熒光探針的設(shè)計與應(yīng)用中提供了廣泛空間。如今,已經(jīng)有很多基于芘及其衍生物的熒光探針被研究開發(fā)出來用于各種生物分子、金屬離子等的檢測。在這些熒光探針中,雖然有些具有較好的靈敏度和選擇性,但還是受到復(fù)雜的合成工序、差的水溶性等缺陷的制約,使其不能在實際應(yīng)用中得以推廣。因此,如何設(shè)計出易于合成、水溶性好、響應(yīng)快速并能夠得到實際應(yīng)用的芘基熒光探針,具有重要的研究意義�；谝陨系脑O(shè)計理念和研究目的,本文主要做了如下工作:1.兩步法合成了陰離子型芘衍生物4-(芘-1-基)丁烷-1-磺酸鈉(PBSS),在不同的緩沖溶液中由聚電解質(zhì)聚二烯基丙二甲基氯化銨(PDDA)在靜電作用力下誘導(dǎo)制備得到激基締合物(PPE),并對其進(jìn)行了光譜性質(zhì)等的研究。2.將激基締合物(PPE)用于對環(huán)境污染物2,4,6-三硝基苯酚(TNP)的檢測。TNP被廣泛用于印染、生物醫(yī)藥、煙花等相關(guān)領(lǐng)域,是一種典型的環(huán)境污染物,對它的相關(guān)研究與檢測也一直備受關(guān)注。本文通過TNP對PPE熒光的淬滅建立了一種新型的TNP快速檢測法。并通過一系列的實驗對機(jī)理進(jìn)行了探究,發(fā)現(xiàn)TNP在靜電作用、π-π作用以及電子轉(zhuǎn)移作用下直接與PPE結(jié)合,并未導(dǎo)致PPE解體�；谶@一獨特的機(jī)理實現(xiàn)了對TNP的高靈敏度、高選擇性、可視化的檢測,檢測限為5 n M。在實際樣品土壤和自來水中,PPE仍舊展現(xiàn)出對TNP很好的檢測效果,檢測限分別為10 n M和22.9 ppb。3.將激基締合物(PPE)用于對生物大分子肝素(heparin)的檢測。heparin是臨床用量最大的抗凝血藥物,同時也與眾多的生理和病理過程息息相關(guān),因此,實時監(jiān)測和量化血清中的heparin,對于生理過程的研究和臨床應(yīng)用具有重要的意義。本文利用heparin誘導(dǎo)芘的excimer-monomer的相互轉(zhuǎn)換導(dǎo)致PPE解離,實現(xiàn)heparin的快速可視化檢測。檢測限為0.14μM。在紫外燈的照射下,肉眼的檢測限為2.0μM。在實際樣品牛血清中檢測heparin時,同樣得到了令人滿意的檢測效果,這對未來heparin的檢測和研究具有重要的參考價值。
[Abstract]:Fluorescent probes have attracted much attention for their advantages such as simple operation, high sensitivity, rapid response, low cost and no damage to samples, and have been widely used in chemical, biological and medical research fields. Pyrene and its derivatives are a kind of fluorescent materials with excellent properties, such as stable chemical structure, easy synthesis, high quantum yield and long fluorescence lifetime. Especially under certain conditions, the conversion between monomer and excimer can occur in pyrene ring, which provides a wide range of space for the design and application of fluorescent probes. Nowadays, many fluorescent probes based on pyrene and its derivatives have been developed for the detection of various biomolecules and metal ions. Although some of these fluorescent probes have good sensitivity and selectivity, they are still restricted by the defects of complex synthesis process and poor water solubility, which make them impossible to be popularized in practical applications. Therefore, it is of great significance to design pyrene fluorescence probes which are easy to synthesize, water-soluble, fast response and can be applied in practice. Based on the above design ideas and research purposes, this paper mainly does the following work: 1. Anion pyrene derivative 4- (pyrene 1-yl) butane-1-sulfonate (PBSS) was synthesized by two-step method. The excimer was induced by polyelectrolyte polyallylidene dimethylammonium chloride (PDDA) in different buffer solution under electrostatic force. (PPE), and its spectral properties were studied. The excimer association complex (PPE) has been widely used in printing and dyeing, biomedicine, fireworks and other related fields. It is a typical environmental pollutant, and its related research and detection has been paid much attention. In this paper, a new rapid determination method of TNP was established by quenching the fluorescence of PPE by TNP. Through a series of experiments, it is found that TNP binds directly to PPE under electrostatic action, 蟺-蟺 interaction and electron transfer, and does not lead to the disintegration of PPE. Based on this unique mechanism, the high sensitivity, high selectivity and visual detection of TNP are realized. The detection limit is 5 nm. The detection limits of PPE for TNP in soil and tap water were 10 mm and 22. 9 ppb. 3, respectively. The use of excimer association complex (PPE) in the detection of biomacromolecular heparin (heparin) is the most widely used anticoagulant, and is closely related to many physiological and pathological processes. Real-time monitoring and quantification of heparin in serum is of great significance for the study of physiological processes and clinical application. In this paper, the conversion of excimer-monomer induced by pyrene by heparin leads to the dissociation of heparin, which realizes the rapid visual detection of heparin. The detection limit was 0.14 渭 M. Under the irradiation of ultraviolet lamp, the detection limit of naked eye was 2.0 渭 M. The detection of heparin in bovine serum is also satisfactory, which has important reference value for the detection and study of heparin in the future.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3

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