本文選題：毛細(xì)管電泳 + 安培檢測(cè)　； 參考：《遼寧師范大學(xué)》2015年碩士論文

【摘要】：毛細(xì)管電泳(Capillary Electrophoresis,CE)是一種在毛細(xì)管中待測(cè)物溶液以高壓電場(chǎng)為驅(qū)動(dòng)力實(shí)現(xiàn)高效快速分離的分離技術(shù)。隨著近年來的發(fā)展,其應(yīng)用的范圍更加廣泛,已經(jīng)應(yīng)用在藥物、食品、生物以及環(huán)境分析等各個(gè)領(lǐng)域。雌酚類激素因能夠促進(jìn)動(dòng)物生長、增加乳產(chǎn)量等作用而被廣泛應(yīng)用于動(dòng)物生產(chǎn),這些激素通過食物鏈,進(jìn)入人體內(nèi),有致癌等毒副作用,包括己烯雌酚、己烷雌酚和雙烯雌酚。目前,單獨(dú)檢測(cè)己烯雌酚和檢測(cè)己烯雌酚及其他雌激素的報(bào)道有很多,而同時(shí)檢測(cè)己烯雌酚、己烷雌酚和雙烯雌酚的報(bào)道還比較少見,同時(shí)檢測(cè)分離這三種物質(zhì)具有很大的意義。本論文基于毛細(xì)管電泳—安培檢測(cè)技術(shù)獨(dú)特的優(yōu)點(diǎn),從新緩沖體系的研究入手,定性定量雌酚類激素及其相關(guān)藥物和魚肉中的殘留成分,使毛細(xì)管電泳-安培檢測(cè)的應(yīng)用范圍更廣泛。本文主要包括以下三個(gè)部分:第一章:緒論該章介紹了毛細(xì)管電泳概況,重點(diǎn)介紹了毛細(xì)管電泳樣品預(yù)富集技術(shù)和在線樣品富集技術(shù),環(huán)糊精的結(jié)構(gòu)和性質(zhì)以及在分析化學(xué)中的應(yīng)用,并簡單的介紹了雌激素殘留的主要途徑及對(duì)環(huán)境和人類的影響,雌激素的研究進(jìn)展,闡述了本文的選題意義。第二章:β-環(huán)糊精修飾膠束電動(dòng)毛細(xì)管色譜法對(duì)雌酚類激素的檢測(cè)本文建立了β-環(huán)糊精修飾膠束毛細(xì)管電泳-安培檢測(cè)聯(lián)用技術(shù)測(cè)定雌酚類激素己烷雌酚(HEX)、己烯雌酚(DES)和雙烯雌酚(DE)殘留的新方法。研究了一種毛細(xì)管電泳-電化學(xué)檢測(cè)(CZE-AD)的新緩沖體系,此體系可在酸性條件下,運(yùn)用β-環(huán)糊精和膠束SDS對(duì)樣品進(jìn)行檢測(cè)。實(shí)驗(yàn)考察了緩沖體系的組成、濃度、分離電壓、檢測(cè)電位及進(jìn)樣時(shí)間等對(duì)實(shí)驗(yàn)結(jié)果的影響。DE、HEX與DES的分別在4×10-5-5×10-4 mol/L,1×10-5-5×10-4mol/L和1×10-5-5×10-4 mol/L范圍內(nèi)呈良好線性,相關(guān)系數(shù)(r2)分別為0.9819、0.9939、0.9867,檢出限為10-6 mol/L。本方法選擇性好,分析速度快,操作簡單,能夠在復(fù)雜混合物中測(cè)定各組分,并得出滿意的結(jié)果。第三章:毛細(xì)管電泳-安培檢測(cè)法測(cè)定動(dòng)物組織中3種雌酚類激素的研究建立了毛細(xì)管區(qū)帶電泳-安培檢測(cè)法同時(shí)對(duì)三種雌酚類激素己烷雌酚(HEX)、己烯雌酚(DES)及雙烯雌酚(DE)的殘留量進(jìn)行測(cè)定。研究了緩沖體系的組成、濃度、分離電壓、檢測(cè)電位以及進(jìn)樣時(shí)間等對(duì)實(shí)驗(yàn)結(jié)果的影響。本方法線性范圍寬,目標(biāo)組分在10-7-10-5mol/L范圍內(nèi)呈良好線性,檢出限(LOD)低,達(dá)到10-9-10-8 mol/L,靈敏度比以往文獻(xiàn)高,且在實(shí)際樣品中也能很好的檢測(cè)。
[Abstract]:Capillary Electrophoresis (CE) is a separation technique for high efficiency and rapid separation of the solution in a capillary with high pressure electric field as the driving force. With the development of recent years, its application has been widely used in various fields, such as drugs, food, raw materials and environmental analysis. It is widely used in animal production to promote animal growth and increase milk production. These hormones enter the human body through the food chain and have the toxic and side effects of carcinogenesis, including diethylstilbestrol, hexbestrol and dienethylstilbestrol. There are many reports of diethylstilbestrol and diethylstilbestrol and other estrogens at the same time. The reports of diethylstilbestrol, hexbestrol and dienethylstilbestrol are relatively rare, and the detection of these three substances is of great significance. Based on the unique advantages of capillary electrophoresis - ampere detection technology, this paper, starting with the study of the new buffer system, qualitatively and quantitatively quantified the residual components in the estradiol and related drugs and fish. The application scope of capillary electrophoresis - ampere detection is more extensive. This article mainly includes the following three parts: Chapter 1: introduction to introduction of the general situation of capillary electrophoresis, focusing on capillary electrophoresis sample preconcentration technology and on-line sample enrichment technology, structure and properties of cyclodextrin, and its application in analytical chemistry, and simple The main ways of estrogen residue and its influence on environment and human were introduced. The research progress of estrogen and the significance of this topic were expounded. The second chapter: beta cyclodextrin modified micellar electrokinetic capillary chromatography for the determination of estrin, the determination of female by beta cyclodextrin modified gel capillary electrophoresis and amperometric detection was established. A new method for the residue of hexenostrol (HEX), diethylstilbestrol (DES) and diethylstilbestrol (DE) of phenolic hormones. A new buffer system for capillary electrophoresis electrochemical detection (CZE-AD) was studied. The system can be detected by beta cyclodextrin and micellar SDS under acidic conditions. The composition, concentration and separation voltage of the buffer system are investigated experimentally. The effect of detection potential and sampling time on the experimental results.DE, HEX and DES are in good linearity in the range of 4 x 10-5-5 x 10-4 mol/L, 1 x 10-5-5 x 10-4mol/L and 1 x 10-5-5 x 10-4 mol/L, and the correlation coefficient (R2) is 0.9819,0.9939,0.9867, the detection limit is 10-6 mol/L. this method is selective, the analysis speed is fast, the operation is simple and can be used. The third chapter: the determination of 3 kinds of estradiol in animal tissues by capillary electrophoresis and amperometric detection established the capillary zone electrophoresis ampere detection method and the residue of three diethylstilbestrolestones (HEX), diethylstilbestrol (DES) and diethylstilbestrol (DE) The influence of the composition of the buffer system, the concentration, the separation voltage, the detection potential and the sampling time on the experimental results is studied. The linear range of the method is wide, the target component is in good linearity in the range of 10-7-10-5mol/L, the detection limit (LOD) is low, 10-9-10-8 mol/L is reached, and the sensitivity is higher than the previous literature, and it can also be good in the actual sample. Testing.
【學(xué)位授予單位】：遼寧師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：O657

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