本文選題：硅橡膠　切入點(diǎn)：氧化石墨烯　出處：《太原理工大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：硅橡膠由于其自身的理化穩(wěn)定性、生理惰性和耐老化等優(yōu)良的特性而廣泛應(yīng)用于植入材料,但其表面易于發(fā)生細(xì)菌黏附,進(jìn)而形成生物膜,引發(fā)感染和多種并發(fā)癥,使病人承受極大的痛苦。在硅橡膠表面制備抗菌涂層是減少這類細(xì)菌感染的簡(jiǎn)單而有效的手段。但常用的抗菌劑如銀離子等抗菌涂層在有效地阻止細(xì)菌黏附、增殖的同時(shí),存在細(xì)胞毒性等潛在危險(xiǎn),其應(yīng)用受到了限制。為此,為獲得安全、有效的抗菌涂層,本研究在硅橡膠基體上經(jīng)化學(xué)改性后將氧化石墨烯接枝在其表面,形成氧化石墨烯抗菌涂層。首先通過紫外輻照對(duì)硅橡膠表面進(jìn)行活化,使其表面產(chǎn)生活性基團(tuán),隨后在其表面接枝硅烷偶聯(lián)劑(?-巰丙基)三甲氧基硅烷,從而使氧化石墨烯與硅橡膠表面牢固鍵和,形成抗菌涂層。為了增大氧化石墨烯比表面積,利用溶致液晶軟模板制備出有序納米結(jié)構(gòu)氧化石墨烯,并將其接枝在硅橡膠基體表面,形成有序結(jié)構(gòu)氧化石墨烯抗菌涂層。利用拉曼光譜(Raman Spectra)、同步輻射小角X-射線散射(SAXS)表征氧化石墨烯微觀結(jié)構(gòu);采用掃描電子顯微鏡(SEM)觀察樣品表面的微觀形貌;通過接觸角測(cè)量?jī)x來觀察樣品表面親疏水性變化;采用偏光顯微鏡(POM)和低角度X-射線衍射(Low-angle XRD)表征溶致液晶模板結(jié)構(gòu);采用低角度XRD和透射電子顯微鏡(TEM)測(cè)定結(jié)構(gòu)化氧化石墨烯的有序結(jié)構(gòu);利用平板菌落計(jì)數(shù)法和熒光染色法評(píng)價(jià)氧化石墨烯涂層及有序結(jié)構(gòu)氧化石墨烯涂層的抗菌性能;通過細(xì)胞活死染色法檢測(cè)細(xì)胞死活,判定氧化石墨烯抗菌涂層對(duì)內(nèi)皮細(xì)胞的細(xì)胞毒性。以上實(shí)驗(yàn)得到的主要結(jié)論有:(1)通過紫外輻照及硅烷偶聯(lián)劑的橋接作用,將氧化石墨烯接枝在硅橡膠基體表面,形成氧化石墨烯涂層。(2)硅橡膠基體表面的氧化石墨烯涂層對(duì)大腸桿菌和金黃色葡萄球菌都表現(xiàn)出了抗菌性,且氧化石墨烯涂層對(duì)大腸桿菌和金黃色葡萄球菌的抗菌性更多地來源于其氧化應(yīng)激作用,而不僅僅是機(jī)械物理損傷。(3)硅橡膠基體表面的氧化石墨烯涂層對(duì)內(nèi)皮細(xì)胞沒有表現(xiàn)出明顯的細(xì)胞毒性。(4)利用溶致液晶軟模板可成功制備出層狀和六方有序結(jié)構(gòu)氧化石墨烯。(5)與硅橡膠基體表面的氧化石墨烯涂層相比,有序結(jié)構(gòu)氧化石墨烯涂層的抗菌性有所增強(qiáng)。
[Abstract]:Silicone rubber is widely used in implant materials because of its physical and chemical stability, physiological inertia and aging resistance. However, bacterial adhesion is easy to occur on the surface of silicone rubber, resulting in biofilm, infection and many complications. The preparation of antibacterial coatings on silicone rubber surfaces is a simple and effective means to reduce such bacterial infections. But common antimicrobial agents such as silver ions can effectively prevent bacteria from adhering and proliferating. In order to obtain a safe and effective antibacterial coating, graphene oxide was grafted onto the surface of silicone rubber matrix after chemical modification. The surface of silicone rubber was first activated by ultraviolet irradiation to produce active groups, and then the silane coupling agent was grafted on the surface. In order to increase the specific surface area of graphene oxide, graphene oxide was prepared by lyotropic liquid crystal soft template. It was grafted on the surface of silicone rubber to form an ordered graphene oxide antibacterial coating. The microstructure of graphene oxide was characterized by Raman Spectra-Raman spectroscopy and SAXS. Scanning electron microscopy (SEM) was used to observe the microstructure of the sample surface, the hydrophobicity of the sample surface was observed by contact angle measuring instrument, the structure of lyotropic liquid crystal template was characterized by polarizing microscope (POM) and low-angle X-ray diffraction (Low-angle XRD). The ordered structure of structured graphene oxide was determined by low angle XRD and transmission electron microscope (TEM), and the antibacterial properties of graphene oxide coating and ordered graphene oxide coating were evaluated by plate colony counting and fluorescence staining. The cytotoxicity of graphene oxide coating on endothelial cells was determined by the method of cell death staining. The main conclusions of the above experiments are: 1) bridging by ultraviolet irradiation and silane coupling agent. Graphene oxide was grafted on the surface of silicone rubber to form graphene oxide coating. 2) the graphene oxide coating on the surface of silicone rubber showed antibacterial activity against Escherichia coli and Staphylococcus aureus. Moreover, the antibacterial activity of graphene oxide coating against Escherichia coli and Staphylococcus aureus was mainly due to its oxidative stress. Not only mechanical and physical damage. (3) graphene oxide coating on silicone rubber substrates showed no obvious cytotoxicity to endothelial cells. 4) layered and hexagonal ordered oxygen could be successfully prepared by using lyotropic liquid crystal soft template. Compared with the graphene oxide coating on the surface of silicone rubber, The antibacterial properties of the ordered graphene oxide coating were improved.
【學(xué)位授予單位】：太原理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TB306;R318.08

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