皮層谷氨酸能和GABA能神經(jīng)元可塑性與學(xué)習(xí)效率相關(guān)
發(fā)布時(shí)間：2018-11-03 16:01
【摘要】：目的:通過(guò)建立的聯(lián)合式學(xué)習(xí)記憶模型小鼠,探究學(xué)習(xí)效率與桶狀皮層中不同類型神經(jīng)元可塑性之間的關(guān)系。方法:1.造模:實(shí)驗(yàn)動(dòng)物主要選用出生20天后的C57Thy1-YFP/GAD67-GFP小鼠(以下簡(jiǎn)稱小鼠),實(shí)驗(yàn)小鼠總共分為兩組,第一組小鼠在以乙酸丁酯作為氣味源刺激訓(xùn)練的同時(shí),機(jī)械擺動(dòng)小鼠右側(cè)胡須,擺動(dòng)頻率為5Hz,持續(xù)時(shí)間為20秒,每隔2小時(shí)訓(xùn)練一次,每天訓(xùn)練5次,連續(xù)訓(xùn)練10天,訓(xùn)練期間,將每只小鼠放在家庭籠中飼養(yǎng)并觀察情況。第二組不同于第一組小鼠的是在給予乙酸丁酯作為氣味源嗅覺(jué)刺激的同時(shí)沒(méi)有胡須擺動(dòng)的刺激條件,而是在嗅覺(jué)條件完成一小時(shí)后再刺激胡須使其周期性擺動(dòng),此為第二組實(shí)驗(yàn)小鼠的一個(gè)訓(xùn)練方法。一天內(nèi)反復(fù)實(shí)驗(yàn)五次,從而形成不配對(duì)的對(duì)照訓(xùn)練組。2.行為監(jiān)測(cè):在每天的訓(xùn)練完成后,拍攝并分析小鼠胡須擺動(dòng)情況,拍攝時(shí)只給予小鼠乙酸丁酯的嗅覺(jué)刺激(嗅覺(jué)誘導(dǎo)的胡須擺動(dòng)情況),隨后用MATLAB軟件進(jìn)行運(yùn)動(dòng)追蹤胡須擺動(dòng)軌跡。依據(jù)胡須的擺動(dòng)軌跡情況對(duì)小鼠學(xué)習(xí)效率進(jìn)行劃分,第一組小鼠產(chǎn)生高效學(xué)習(xí)組、低效學(xué)習(xí)組,第二組小鼠為不對(duì)稱訓(xùn)練對(duì)照組。3.電生理實(shí)驗(yàn):聯(lián)合式訓(xùn)練第6天時(shí)對(duì)各組小鼠分別進(jìn)行電生理實(shí)驗(yàn),本實(shí)驗(yàn)主要對(duì)小鼠腦內(nèi)barrel區(qū)的谷氨酸能神經(jīng)元和GABA能神經(jīng)元的編碼能力和突觸傳遞效率這兩類指標(biāo)的變化進(jìn)行著重考察和分析。結(jié)果:1.行為學(xué)結(jié)果:(1)高效學(xué)習(xí)組與低效學(xué)習(xí)組相比,小鼠胡須擺動(dòng)頻率增加(P0.05),角度增加(P0.05);(2)低效學(xué)習(xí)組與不對(duì)稱訓(xùn)練組,小鼠胡須擺動(dòng)頻率增加(P0.01),角度增加(P0.01);(3)高效學(xué)習(xí)組與不對(duì)稱訓(xùn)練組,小鼠胡須擺動(dòng)頻率顯著增加(P0.001),角度顯著增加(P0.001)。2.電生理實(shí)驗(yàn)結(jié)果:(1)谷氨酸能神經(jīng)元實(shí)驗(yàn)結(jié)果:1)高效學(xué)習(xí)組與低效學(xué)習(xí)組相比,小鼠大腦桶狀皮層谷氨酸能神經(jīng)元在單位時(shí)間內(nèi)動(dòng)作電位發(fā)放數(shù)量增加(P0.05),s EPSC(自發(fā)性興奮性突觸后電流)的電流發(fā)放間距減小(P0.05)、幅度增加(P0.05),s IPSC(自發(fā)性抑制性突觸后電流)的電流發(fā)放間距增加(P0.05)、幅度減小(P0.05);2)低效學(xué)習(xí)組與不對(duì)稱訓(xùn)練組相比,小鼠大腦桶狀皮層的單位時(shí)間內(nèi)動(dòng)作電位發(fā)放個(gè)數(shù)增加(P0.051),s EPSC(自發(fā)性興奮性突觸后電流)的電流發(fā)放間距減小(P0.01)、幅度增加(P0.01),s IPSC(自發(fā)性抑制性突觸后電流)的電流發(fā)放間距增加(P0.01)、幅度減小(P0.01);3)高效學(xué)習(xí)組與不對(duì)稱訓(xùn)練組相比,小鼠大腦桶狀皮層谷氨酸能神經(jīng)元在單位時(shí)間內(nèi)動(dòng)作電位發(fā)放個(gè)數(shù)增加(P0.001),s EPSC(自發(fā)性興奮性突觸后電流)的發(fā)放間距減小(P0.001)、幅度增加(P0.001),s IPSC(自發(fā)性抑制性突觸后電流)的發(fā)放間距增加(P0.001)、幅度減小(P0.001)。(2)GABA能神經(jīng)元實(shí)驗(yàn)結(jié)果:1)高效學(xué)習(xí)組與低效學(xué)習(xí)組相比,小鼠大腦桶狀皮層GABA能神經(jīng)元在單位時(shí)間內(nèi)動(dòng)作電位發(fā)放個(gè)數(shù)減少(P0.05),s EPSC(自發(fā)性興奮性突觸后電流)的電流發(fā)放間距增加(P0.05)、幅度減小(P0.05);2)低效學(xué)習(xí)組與不對(duì)稱訓(xùn)練組相比,小鼠大腦桶狀皮層GABA能神經(jīng)元在單位時(shí)間內(nèi)動(dòng)作電位發(fā)放個(gè)數(shù)減少(P0.01),s EPSC(自發(fā)性興奮性突觸后電流)的電流發(fā)放間距增加(P0.01)、幅度減小(P0.01);3)高效學(xué)習(xí)組與不對(duì)稱訓(xùn)練組相比,小鼠大腦桶狀皮層GABA能神經(jīng)元在單位時(shí)間內(nèi)動(dòng)作電位發(fā)放個(gè)數(shù)減少(P0.001),s EPSC(自發(fā)性興奮性突觸后電流)的電流發(fā)放間距增加(P0.001)、幅度減小(P0.001)。結(jié)論:配對(duì)的胡須和氣味刺激能夠建立嗅覺(jué)誘導(dǎo)的胡須擺動(dòng)情況,且出現(xiàn)高學(xué)習(xí)效率和低學(xué)習(xí)效率。學(xué)習(xí)效率與桶狀皮層不同類型神經(jīng)元可塑性變化相關(guān)。與未配對(duì)訓(xùn)練組相比,配對(duì)訓(xùn)練組的小鼠桶狀皮層谷氨酸能神經(jīng)元功能上調(diào),GABA能神經(jīng)元功能下調(diào);且學(xué)習(xí)效率高的小鼠這種上調(diào)和下調(diào)比學(xué)習(xí)效率低的小鼠更顯著。
[Abstract]:Objective: To explore the relationship between learning efficiency and plasticity of different types of neurons in barrel-shaped cortex by establishing a mouse model of combined learning and memory model. Method: 1. Model: The experimental animals were randomly divided into two groups: the first group of mice were randomly divided into two groups: the first group of mice stimulated the training with butyl acetate as the odor source, the right beards of the mice were shaken, the swing frequency was 5Hz, and the duration was 20 seconds. Each mouse was trained once every 2 hours, five times a day, 10 days of continuous training, and each mouse was kept and observed in a family cage during training. The second group is different from the first group of mice, which is a training method for the second group of experimental mice after the stimulation condition that butyl acetate is administered as an odor source olfactory stimulus without a beard swing, but is stimulated periodically after the olfactory condition is completed for one hour. Repeat the experiment five times a day to form an unpaired control training group. Behavior monitoring: After each day's training, shoot and analyze the rocking condition of the mouse's beard. Only the olfactory stimuli of butyl acetate (smell induced beard swing) were given during shooting, and then the trajectory of beard swing was traced with MATLAB software. The learning efficiency of the mice was divided on the basis of the swinging locus of the beard. The first group of mice produced the high-efficiency learning group and the inefficient learning group. The second group of mice was the asymmetric training control group. Electrophysiological experiment: Electrophysiological experiments were carried out on each group of mice on the sixth day of combined training. The experiment was mainly focused on the changes of the coding ability and synaptic transmission efficiency of glutamate neurons and GABA-energy neurons in the barrel region of mice. Result: 1. Results: (1) Compared with inefficient learning group, the swinging frequency of beard of mice increased (P 0.05), the angle increased (P 0.05), (2) inefficient learning group and asymmetric training group increased (P0.01), and the angle increased (P0.01). (3) The swinging frequency of beard of mice increased significantly (P0.001) and the angle was increased significantly (P0.001) in the high efficiency learning group and the asymmetric training group (P0.001). The results of electrophysiological experiments: (1) The experimental results of glutamate neurons: 1) Compared with inefficient learning group, the number of action potentials of glutamate neurons in barrel-shaped cortex of mice increased in unit time (P0.05). The current distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P 0.05), the current distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.05), and the amplitude decreased (P0.05). The number of action potential distribution in the barrel cortex of mouse brain increased (P 0.01), and the current distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P0.01), and the amplitude was increased (P0.01). The current distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.01), and the amplitude was decreased (P0.01); 3) The number of action potentials (P0.001) was increased in barrel-shaped cortical glutamate neurons in mouse brain compared with asymmetric training group (P <0.01). The distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P0. 001), the distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.001), and the amplitude decreased (P0.001). (2) GABA-energy neuron experiment results: 1) Compared with inefficient learning group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potential distribution in unit time (P0.05), and the current distribution interval of s EPSC (spontaneous excitable synaptic current) increased (P0.05). Compared with asymmetric training group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potentials in unit time (P0.01), and the current distribution interval of s EPSC (spontaneous excitability postsynaptic current) increased (P0.01). Compared with asymmetric training group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potentials (P0.001) in unit time compared with asymmetric training group (P <0.01), and the current distribution interval of s EPSC (spontaneous excitable synaptic current) increased (P <0.001). Amplitude decreased (P0.001). Conclusion: Paired beard and odor stimulation can establish olfactory induced beard swing and high learning efficiency and low learning efficiency. The learning efficiency was related to the plasticity of different types of neurons in barrel cortex. Compared with the unpaired training group, the mouse barrel-shaped cortical glutamate neuron function of the paired training group is up-regulated, the function of GABA can be down-regulated, and the mouse with high learning efficiency is more remarkable than the mice with low learning efficiency.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R338
本文編號(hào)：2308256
[Abstract]:Objective: To explore the relationship between learning efficiency and plasticity of different types of neurons in barrel-shaped cortex by establishing a mouse model of combined learning and memory model. Method: 1. Model: The experimental animals were randomly divided into two groups: the first group of mice were randomly divided into two groups: the first group of mice stimulated the training with butyl acetate as the odor source, the right beards of the mice were shaken, the swing frequency was 5Hz, and the duration was 20 seconds. Each mouse was trained once every 2 hours, five times a day, 10 days of continuous training, and each mouse was kept and observed in a family cage during training. The second group is different from the first group of mice, which is a training method for the second group of experimental mice after the stimulation condition that butyl acetate is administered as an odor source olfactory stimulus without a beard swing, but is stimulated periodically after the olfactory condition is completed for one hour. Repeat the experiment five times a day to form an unpaired control training group. Behavior monitoring: After each day's training, shoot and analyze the rocking condition of the mouse's beard. Only the olfactory stimuli of butyl acetate (smell induced beard swing) were given during shooting, and then the trajectory of beard swing was traced with MATLAB software. The learning efficiency of the mice was divided on the basis of the swinging locus of the beard. The first group of mice produced the high-efficiency learning group and the inefficient learning group. The second group of mice was the asymmetric training control group. Electrophysiological experiment: Electrophysiological experiments were carried out on each group of mice on the sixth day of combined training. The experiment was mainly focused on the changes of the coding ability and synaptic transmission efficiency of glutamate neurons and GABA-energy neurons in the barrel region of mice. Result: 1. Results: (1) Compared with inefficient learning group, the swinging frequency of beard of mice increased (P 0.05), the angle increased (P 0.05), (2) inefficient learning group and asymmetric training group increased (P0.01), and the angle increased (P0.01). (3) The swinging frequency of beard of mice increased significantly (P0.001) and the angle was increased significantly (P0.001) in the high efficiency learning group and the asymmetric training group (P0.001). The results of electrophysiological experiments: (1) The experimental results of glutamate neurons: 1) Compared with inefficient learning group, the number of action potentials of glutamate neurons in barrel-shaped cortex of mice increased in unit time (P0.05). The current distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P 0.05), the current distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.05), and the amplitude decreased (P0.05). The number of action potential distribution in the barrel cortex of mouse brain increased (P 0.01), and the current distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P0.01), and the amplitude was increased (P0.01). The current distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.01), and the amplitude was decreased (P0.01); 3) The number of action potentials (P0.001) was increased in barrel-shaped cortical glutamate neurons in mouse brain compared with asymmetric training group (P <0.01). The distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P0. 001), the distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.001), and the amplitude decreased (P0.001). (2) GABA-energy neuron experiment results: 1) Compared with inefficient learning group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potential distribution in unit time (P0.05), and the current distribution interval of s EPSC (spontaneous excitable synaptic current) increased (P0.05). Compared with asymmetric training group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potentials in unit time (P0.01), and the current distribution interval of s EPSC (spontaneous excitability postsynaptic current) increased (P0.01). Compared with asymmetric training group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potentials (P0.001) in unit time compared with asymmetric training group (P <0.01), and the current distribution interval of s EPSC (spontaneous excitable synaptic current) increased (P <0.001). Amplitude decreased (P0.001). Conclusion: Paired beard and odor stimulation can establish olfactory induced beard swing and high learning efficiency and low learning efficiency. The learning efficiency was related to the plasticity of different types of neurons in barrel cortex. Compared with the unpaired training group, the mouse barrel-shaped cortical glutamate neuron function of the paired training group is up-regulated, the function of GABA can be down-regulated, and the mouse with high learning efficiency is more remarkable than the mice with low learning efficiency.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R338
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