【摘要】：近年來水污染、食品安全、疾病健康等問題日益突出,嚴(yán)重威脅著人類的健康。傳統(tǒng)檢測方法存在儀器體積較大、價格昂貴、操作復(fù)雜等問題,因此發(fā)展一種低成本、高靈敏度的生化檢測技術(shù)成為當(dāng)前的研究熱點之一。背向散射干涉(Back-scattering interferometry,BSI)技術(shù)具有免標(biāo)記、所需樣品量小、檢測限低、靈敏度高等優(yōu)點,有望應(yīng)用于食品檢測、環(huán)境監(jiān)測、臨床診斷以及生化分析等領(lǐng)域中。將激光入射到通有待測物質(zhì)的毛細管內(nèi),光在毛細管內(nèi)外界面多角度的反射和折射,在環(huán)繞管軸360°范圍內(nèi)產(chǎn)生了干涉條紋,人們將其形象地稱為“背向散射干涉”。當(dāng)管內(nèi)液體的折射率改變時,干涉條紋花樣不變,但相對于初始干涉條紋有一定移動,根據(jù)移動量與折射率變化間關(guān)系,可實現(xiàn)折射率變化、抗原-抗體等分子間作用的測量。本文在BSI技術(shù)基礎(chǔ)上,結(jié)合溫度控制和快速傅里葉變換,搭建了一套基于BSI的高靈敏度定量分析與檢測系統(tǒng),并實現(xiàn)了微體積非吸光物質(zhì)高靈敏度的定量檢測;由于吸光物質(zhì)吸收激光會產(chǎn)生光熱效應(yīng),并引起溶液折射率的改變,因而該系統(tǒng)還可以用于微體積吸光物質(zhì)的高靈敏定量檢測。本文主要研究內(nèi)容如下:1、采用溫度控制系統(tǒng)和保溫裝置,實現(xiàn)了待測樣品所處環(huán)境的溫度的精確控制,使得檢測過程中,樣品不受周圍環(huán)境溫度波動的影響,實驗過程中溫度的波動僅有±0.007℃,提高了檢測的分辨率和系統(tǒng)的穩(wěn)定性,并通過Lab VIEW實現(xiàn)了數(shù)據(jù)的實時采集和自動化處理。2、通過對不同濃度的甘油和不同溫度超純水的檢測,驗證了該檢測系統(tǒng)對非吸光物質(zhì)的高靈敏度定量檢測性能�；诩t墨水溶液中的光熱效應(yīng),對不同濃度品紅墨水的光熱信號進行了測量,結(jié)果表明該檢測系統(tǒng)可用于對吸光物質(zhì)的定量檢測。3、過量的亞硝酸鹽對人體健康具有極大的危害,因此對亞硝酸鹽的定量檢測具有重要的意義。利用改進的格里斯重氮化反應(yīng),使得亞硝酸鹽通過顯色反應(yīng)轉(zhuǎn)變?yōu)樽霞t色偶氮化合物,該化合物吸收綠色激光會產(chǎn)生明顯的光熱效應(yīng)。采用波長為532 nm的激光作為激發(fā)光源,利用該化合物溶液的光熱效應(yīng)和BSI技術(shù)實現(xiàn)了亞硝酸鹽濃度的定量檢測;對自來水及加標(biāo)自來水樣中的亞硝酸鹽進行了檢測,并與紫外-可見光分光光度法的檢測結(jié)果進行了對比,結(jié)果表明基于光熱效應(yīng)和BSI技術(shù)可實現(xiàn)亞硝酸鹽的高靈敏度定量檢測,使用3σ方法得到檢測限為0.05 mg/L。
[Abstract]:In recent years, water pollution, food safety, disease health and other problems have become increasingly prominent, serious threats to human health. Traditional detection methods have many problems, such as large volume, high price and complex operation. Therefore, the development of a low cost and high sensitivity biochemical detection technology has become one of the current research hotspots. Back-scattering interferometry (BSI) has the advantages of low sample size, low detection limit and high sensitivity. It is expected to be used in food detection, environmental monitoring, clinical diagnosis and biochemical analysis. When the laser is incident into the capillary tube of the material to be measured, the reflection and refraction of light at various angles at the inner and outer interface of the capillary produces interference fringes in 360 擄range around the tube axis, which is vividly referred to as "backscattering interference". When the refractive index of liquid in the tube is changed, the pattern of interference fringes is invariable, but there is a certain movement relative to the initial interference fringes. According to the relationship between the amount of movement and the change of refractive index, the measurement of refractive index change and the interaction between antigen-antibody and other molecules can be realized. In this paper, based on BSI technology and temperature control and fast Fourier transform, a high sensitivity quantitative analysis and detection system based on BSI is built, and the quantitative detection of micro-volume non-absorbent material with high sensitivity is realized. The system can also be used for the sensitive and quantitative detection of microvolume absorbent materials because of the photothermal effect and the change of the refractive index of the solution when the absorbent laser absorbs the light. The main contents of this paper are as follows: 1. The temperature control system and the insulation device are used to realize the accurate control of the temperature of the environment in which the samples are to be tested, so that the samples are not affected by the ambient temperature fluctuations during the detection process. The temperature fluctuation in the experiment is only 鹵0.007 鈩，

本文編號：2192633