發(fā)布時(shí)間：2018-08-19 07:19

【摘要】：血脂異常易誘發(fā)心梗、冠心病和腦卒中等心腦血管疾病。因此,研發(fā)一種預(yù)防和緩解血脂異常的功能性食品是非常必要的。本研究以課題組自行分離的高效降膽固醇植物乳桿菌M1-UVs29為出發(fā)菌株,以植物乳桿菌M1-UVs29和米糠油為主要功效成分,研制一款新型降脂軟膠囊,并通過(guò)體外與體內(nèi)試驗(yàn),對(duì)降脂軟膠囊進(jìn)行功能性評(píng)價(jià),主要研究結(jié)果如下:(1)依次利用Plackett-Burman,最陡爬坡和Box-Benhnken對(duì)植物乳桿菌M1-UVs29冷凍干燥保護(hù)劑成分進(jìn)行優(yōu)化。確定保護(hù)劑的最佳配比為:脫脂乳13.00%(w/v),海藻糖7.60%(w/v),山梨醇2.70%(w/v),該條件下菌粉存活率達(dá)86.28%。添加和未添加保護(hù)劑的植物乳桿菌M1-UVs29,在模擬人工胃液、人工腸液、儲(chǔ)存時(shí)間和熱處理?xiàng)l件下的存活能力差異顯著(P0.05),表明該保護(hù)劑具有較好的保護(hù)能力。(2)將經(jīng)過(guò)保護(hù)劑包埋的植物乳桿菌M1-UVs29和米糠油結(jié)合制成具有降脂功能的軟膠囊。進(jìn)行單因素優(yōu)化確定降脂軟膠囊內(nèi)容物最佳配比為:植物乳桿菌菌粉14.00%、低聚果糖14.00%、米糠油69.97%、蜂蠟2.00%和抗氧化劑0.03%,該軟膠囊感官指標(biāo)、理化指標(biāo)、衛(wèi)生指標(biāo)、加速試驗(yàn)和穩(wěn)定性試驗(yàn)符合質(zhì)量標(biāo)準(zhǔn),說(shuō)明該配方符合降脂軟膠囊工業(yè)化生產(chǎn)要求。(3)降脂軟膠囊體外功能評(píng)價(jià)。以軟膠囊內(nèi)容物體外降膽固醇以及脂肪吸附能力作為檢測(cè)指標(biāo),結(jié)果表明:體外降膽固醇能力最高可達(dá)到59.23%,脂肪吸附能力最高可達(dá)到21.14%,說(shuō)明該軟膠囊內(nèi)容物在體外試驗(yàn)中可以起到降脂、降膽固醇的功效。(4)降脂軟膠囊體內(nèi)功能評(píng)價(jià)。以該軟膠囊內(nèi)容物作為原料,C57BL/6J小鼠為動(dòng)物試驗(yàn)對(duì)象。以降脂軟膠囊PH、PM、PL組與FAT組相比,通過(guò)對(duì)C57BL/6J小鼠的體重、各部分脂肪與體重比、內(nèi)臟與體重比、腸道菌群、血清中TC、TG、LDL、HDL、血清中INS、TNF-α、TBARS、肝臟中TG、肝臟中FAS和CPT-1等指標(biāo)分析,結(jié)果表明降脂軟膠囊各劑量組與FAT組相比,差異顯著(P0.05),肝臟組織病理分析發(fā)現(xiàn)該降脂軟膠囊可以改善高脂飲食造成的血脂異常癥狀。綜上所述,該降脂軟膠囊配方合理,植物乳桿菌M1-UVs29活菌數(shù)符合國(guó)家質(zhì)量標(biāo)準(zhǔn),穩(wěn)定性良好,具有預(yù)防和改善高血脂的作用。
[Abstract]:Dyslipidemia can easily induce myocardial infarction, coronary heart disease and stroke and other cardiovascular and cerebrovascular diseases. Therefore, it is necessary to develop a functional food to prevent and alleviate dyslipidemia. In this study, a new antilipidemic soft capsule was developed with Lactobacillus plantarum M1-UVs29 isolated by our team as the starting strain, Lactobacillus plantarum M1-UVs29 and rice bran oil as the main functional components, and tested in vitro and in vivo. The main results were as follows: (1) Plackett-Burman, steepest climbing slope and Box-Benhnken were used to optimize the composition of M1-UVs29 freeze-drying protectant for Lactobacillus plantarum. The optimum ratio of protective agent was determined as follows: skim milk 13.00% (w / v), trehalose 7.60% (w / v), sorbitol 2.70% (w / v), and the survival rate of bacteria powder reached 86.28%. Lactobacillus plantarum M1-UVs29, added and not added, in simulated artificial gastric juice, artificial intestinal fluid, There were significant differences between storage time and survival ability under heat treatment (P0.05), which indicated that the protective agent had better protective ability. (2) soft capsules with lipid-lowering function were made from Lactobacillus plantarum M1-UVs29 and rice bran oil. The optimum proportion of contents of Jiangzhi soft capsule was determined as follows: Lactobacillus plantarum powder 14.00, fructose 14.00, rice bran oil 69.97, beeswax 2.00% and antioxidant 0.03. The accelerated test and stability test accord with the quality standard, which shows that the formula meets the requirement of industrial production of Jiangzhi soft capsule. (3) the function evaluation of Jiangzhi soft capsule in vitro. The content of soft capsule was used as the index of cholesterol lowering and fat adsorption in vitro. The results showed that the ability of lowering cholesterol in vitro was the highest 59.23 and the ability of fat adsorption was 21.14. The results showed that the content of the soft capsule could reduce lipid and reduce cholesterol in vitro. (4) the function of Jiangzhi soft capsule in vivo was evaluated. The soft capsule contents were used as raw material for C57BL / 6J mice. Compared with FAT group, the weight of C57BL/6J mice, the ratio of fat to body weight, the ratio of viscera to body weight, intestinal flora, serum TCG-TGG-LDLL-HDL, serum INSTNF- 偽 TBARS, liver, FAS and CPT-1 were analyzed. The results showed that there were significant differences between the different dosage groups of Jiangzhi soft capsule and FAT group (P0.05). The pathological analysis of liver tissue showed that Jiangzhi soft capsule could improve the hyperlipidemia symptoms caused by high-fat diet. To sum up, the formula of Jiangzhi soft capsule is reasonable, the number of live bacteria of Lactobacillus plantarum M1-UVs29 accords with the national quality standard, the stability is good, and it has the function of preventing and improving hyperlipidemia.
【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：TS201.3;TS218

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