本文選題：海馬 + 自發(fā)活動�。� 參考：《華東師范大學》2017年碩士論文

【摘要】：海馬是哺乳動物大腦邊緣系統(tǒng)的重要組成部分,在學習和記憶、空間定位等功腦能中起到重要作用。目前普遍認為,海馬功能的實現(xiàn)與海馬神經(jīng)元活動的同步性關系密切。對海馬同步性活動的神經(jīng)機制已有較多的研究,但這一問題尚待進一步明確。本論文采用幼年大鼠的離體全海馬標本,對海馬CA1同步性活動的海馬內(nèi)部環(huán)路機制進行了研究。在該離體全海馬標本中,我們通過胞外場電位記錄可觀察到CA1內(nèi)部活動具有高度同步性。接下來,我們從CA3側(cè)開始,沿垂直于海馬長軸方向切割開3/4的海馬組織,在切割口兩側(cè)所記錄的CA1場電位活動在1小時內(nèi)仍然顯著同步,維持在未作切割的完整海馬組水平;然而其活動的同步不能長期維持,在3小時和5小時開展的記錄中不能見到明顯的同步性活動。另外,我們從海馬CA1側(cè)開始,沿垂直于海馬長軸方向切割開3/4的海馬組織,在切割口兩側(cè)記錄CA1場電位,發(fā)現(xiàn):切開1小時內(nèi)CA1場電位的同步性活動消失;然而,在切開3小時后可觀察到CA1活動的同步性得到明顯恢復,至5小時同步性回到完整海馬水平。為進一步觀察從CA1方向切割海馬后,CA1同步性從消失至恢復的過程,我們接下來采用雙膜片鉗的方法,觀察CA1神經(jīng)元之間的同步性活動,與場電位的實驗結(jié)果類似:在切割兩小時內(nèi)所記錄到的CA1細胞間的膜電位活動的同步性消失,4小時之后開展的記錄可見同步性恢復到接近正常未切割的水平。本論文的研究顯示:離體全海馬CA1腦區(qū)的同步性依賴于CA1內(nèi)部及CA3環(huán)路的完整性。并且,海馬CA1內(nèi)部部分連接的存在可以在短時間內(nèi)維持CA1同步性活動,而其同步性的長期維持需要CA3輸入的完整;同時,海馬CA3完整的輸入可以使得CA1內(nèi)部連接斷開時能逐步恢復CA1活動的同步。這些結(jié)果為進一步理解海馬CA1同步性活動的環(huán)路機制提供了新的數(shù)據(jù)。
[Abstract]:Hippocampus is an important part of mammalian limbic system, which plays an important role in learning and memory, spatial localization and other functional brain functions. It is generally believed that the realization of hippocampal function is closely related to the synchronism of hippocampal neuron activity. The neural mechanism of hippocampal synchronous activity has been studied, but this problem needs to be further clarified. In this paper, the mechanism of hippocampal loop of hippocampal CA1 synchronous activity was studied using isolated hippocampal specimens of young rats. In this isolated hippocampal specimen, we observed that the internal activity of CA1 was highly synchronous by recording extracellular field potential. Next, we cut 3 / 4 of hippocampal tissue perpendicular to the hippocampal long axis from the CA3 side. The CA1 field potential activity recorded on both sides of the incisor was still significantly synchronized within 1 hour, and maintained at the level of intact hippocampal group without cutting. However, the synchronization of its activities can not be maintained for a long time, and no obvious synchronous activities can be observed in the records of 3 and 5 hours. In addition, we cut 3 / 4 of the hippocampal tissue perpendicular to the hippocampal long axis from the hippocampal CA1 side. We recorded CA1 field potentials on both sides of the incised orifice and found that the synchronous activity of CA1 field potentials disappeared within 1 hour of incision; however, It was observed that the synchronization of CA1 activity recovered significantly 3 hours after incision and returned to the intact hippocampal level at 5 hours. In order to further observe the process from disappearance to recovery of CA1 synchrony after hippocampal incising in the direction of CA1, we then used double patch clamp method to observe the synchronous activity between CA1 neurons. The results were similar to those of the field potential experiment: the synchronism of membrane potential activity between CA1 cells recorded within two hours of cutting was disappeared 4 hours later, and the synchronicity of the recording was restored to the normal level of uncleavage. The present study shows that the synchronism of the isolated hippocampal CA1 brain depends on the integrity of the CA 1 internal and CA 3 loop. Moreover, the existence of the internal partial connections in hippocampal CA1 can maintain the synchronous activity of CA1 for a short period of time, and the long-term maintenance of the synchronization requires the integrity of CA3 input. The complete input of hippocampal CA3 can gradually restore the synchronization of CA1 activity when the internal connection of CA1 is disconnected. These results provide new data for further understanding the loop mechanism of hippocampal CA1 synchronism.
【學位授予單位】：華東師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：Q42

【參考文獻】

相關期刊論文 前1條

1 Yang Zhan;;Harnessing GABAergic Transmission for Slow Oscillations[J];Neuroscience Bulletin;2016年05期

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本文編號：2071649