本文選題：前胸腺 + 轉(zhuǎn)錄組��； 參考：《沈陽農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：前胸腺是昆蟲體內(nèi)最重要的內(nèi)分泌器官之一,合成和分泌的蛻皮激素在調(diào)控昆蟲生長(zhǎng)發(fā)育過程中起著關(guān)鍵作用。然而,我們對(duì)這一重要內(nèi)分泌器官在分子水平的認(rèn)識(shí)還相當(dāng)有限,原因在于前胸腺基因組信息的匱乏。為了填補(bǔ)這一缺憾,本論文以兩種重要的經(jīng)濟(jì)昆蟲(家蠶和柞蠶)為材料,利用高通量測(cè)序技術(shù),對(duì)這兩種昆蟲前胸腺的轉(zhuǎn)錄組進(jìn)行了比較。同時(shí),基于轉(zhuǎn)錄組數(shù)據(jù),在前胸腺中鑒定出了一整套的生物鐘基因。此外,對(duì)柞蠶轉(zhuǎn)錄組數(shù)據(jù)中的SSR位點(diǎn)信息進(jìn)行了分析。本論文是首次進(jìn)行昆蟲前胸腺的比較轉(zhuǎn)錄組分析,為深入理解這一重要內(nèi)分泌器官的特征和功能提供非常有價(jià)值的信息資源。主要結(jié)果如下:1.柞蠶和家蠶前胸腺的轉(zhuǎn)錄組測(cè)序和比較分析。拼接組裝后,柞蠶得到64,301個(gè)transcripts(平均長(zhǎng)度 657 bp)和 44,067 個(gè) Unigenes。家蠶得到 49,287 個(gè) transcripts(平均長(zhǎng)度1,064 bp)和32,302個(gè)Unigenes。柞蠶和家蠶數(shù)據(jù)均已儲(chǔ)存在NCBI Sequence Read Archive中,登錄號(hào)分別為SRR5119598和SRR5119600。柞蠶和家蠶中分別有22,402個(gè)(50.84%)和 23,157 個(gè)(71.69%)Unigenes 具有匹配的 homologous hits。比較分析發(fā)現(xiàn),這兩個(gè)前胸腺在GO terms、COG category和KO assignments的功能注釋結(jié)果是高度相似的。另外,兩個(gè)前胸腺轉(zhuǎn)錄組數(shù)據(jù)中,共鑒定出6,357對(duì)直系同源基因。2.生物鐘基因的鑒定和RT-PCR檢測(cè)。生物鐘在昆蟲的行為和生理過程中起著重要調(diào)控作用。從柞蠶和家蠶前胸腺轉(zhuǎn)錄組數(shù)據(jù)中,我們鑒定出了 13個(gè)生物鐘基因,包括cryptochrome 1、cryptochrome 2、period、timeless、clock、cycle、shaggy、double-time、vrille、PAR-domainprotein 1、slimb、casein kinase Ⅱalpha、casein kinase Ⅱbetaa。RT-PCR檢測(cè)結(jié)果表明,這些基因在前胸腺中均有表達(dá)。這些結(jié)果表明,在昆蟲前胸腺中也存在一個(gè)外圍生物鐘。3.柞蠶SSR位點(diǎn)信息分析。在柞蠶轉(zhuǎn)錄組數(shù)據(jù)中,共鑒定出2,322個(gè)SSR位點(diǎn),分布于1,615條Unigenes中,發(fā)生頻率為37.60%,平均分布距離為4.76 kb。柞蠶SSR中,以單昔酸重復(fù)占主導(dǎo)地位。獲得的轉(zhuǎn)錄組數(shù)據(jù)為SSR引物設(shè)計(jì)提供了信息依據(jù),進(jìn)一步為柞蠶遺傳多樣性分析、功能基因發(fā)掘、遺傳圖譜構(gòu)建等的奠定了基礎(chǔ)。
[Abstract]:Prothymus is one of the most important endocrine organs in insects. Ecdysone synthesized and secreted plays a key role in the regulation of insect growth and development. However, our understanding of this important endocrine organ at the molecular level is rather limited, due to the lack of information on the genome of the prethymus. In order to fill this shortcoming, two important economic insects (Bombyx mori and Antheraea pernyi) were used in this paper to compare the transcriptome of prethymus using high-throughput sequencing technique. At the same time, a whole set of biological clock genes were identified in the prethymus based on transcriptional data. In addition, SSR site information in transcriptional data of tussah was analyzed. This paper is the first time to carry out comparative transcriptome analysis of insect prethymus to provide valuable information resources for further understanding the characteristics and functions of this important endocrine organ. The main results are as follows: 1. Transcriptome sequencing and comparative analysis of prethymus of tussah and Bombyx mori. After stitching and assembling, 641 transcripts (average length 657 BP) and 44067 Unigenes were obtained from tussah silkworm (Antheraea pernyi). Bombyx mori obtained 49287 transcripts (average length 1064 BP) and 32302 Unigenes. The data of Antheraea pernyi and Bombyx mori have been stored in NCBI sequence read Archive, and the login numbers are SRR5119598 and SRR5119600respectively. In tussah silkworm and Bombyx mori, there are 22402 and 71.69 Unigenes, respectively. A comparative analysis showed that the functional annotation results of the two prothymocytes in go termscog category and KO assignments were highly similar. In addition, 6357 pairs of homologous genes. 2. 2 were identified from two pre thymus transcriptional data. Identification of biological clock gene and RT-PCR detection. Biological clock plays an important role in insect behavior and physiological processes. From transcriptional data of prethymus of Antheraea pernyi and Bombyx mori, we have identified 13 clock genes, including cryptochrome 1 Cryptochrome 2 (cryptochrome 1) Cryptochrome 2 (cryptochrome 2) and clock. The results of double-time detection of PAR-domainprotein 1 and kinase 鈪，

本文編號(hào)：2028069