本文選題：海兔 + 位置移動(dòng)�。� 參考：《南京大學(xué)》2017年碩士論文

【摘要】：神經(jīng)活動(dòng)的蠕動(dòng)波(rolling wave)所引起的位置移動(dòng)是廣泛存在于脊椎和無(wú)脊椎動(dòng)物中的節(jié)律性運(yùn)動(dòng),而控制這種運(yùn)動(dòng)的神經(jīng)環(huán)路被稱為中樞模式發(fā)生器。在海兔(Aplysia californica)中,以蠕動(dòng)波所產(chǎn)生的逃跑位置移動(dòng)行為是研究位置移動(dòng)中樞模式發(fā)生器的絕佳模型。與那些由多個(gè)體節(jié)神經(jīng)網(wǎng)絡(luò)或體節(jié)神經(jīng)節(jié)調(diào)控的位置移動(dòng)不同,海兔的中樞模式發(fā)生器存在于單一的腳神經(jīng)節(jié)中。根據(jù)先前的研究,我們將工作的重點(diǎn)放在了腹側(cè)腳神經(jīng)節(jié)上。我們用色素回填定位了右側(cè)腳神經(jīng)節(jié)腹面p1神經(jīng)根部的一個(gè)細(xì)胞團(tuán),并且用半離體實(shí)驗(yàn)確認(rèn)了它們可以引起肌肉收縮,進(jìn)一步定性為運(yùn)動(dòng)神經(jīng)元。用電生理記錄的方法同時(shí)進(jìn)行細(xì)胞內(nèi)記錄和細(xì)胞外神經(jīng)記錄,發(fā)現(xiàn)了運(yùn)動(dòng)神經(jīng)元興奮發(fā)放模式與運(yùn)動(dòng)指示器PPCN運(yùn)動(dòng)神經(jīng)存在時(shí)相差,并將所記錄的細(xì)胞與PPCN的時(shí)相關(guān)系、細(xì)胞間電偶聯(lián)做了總結(jié),發(fā)現(xiàn)時(shí)相相近的運(yùn)動(dòng)神經(jīng)元互相電偶聯(lián)。同時(shí)在p7根部記錄到與單一時(shí)相運(yùn)動(dòng)神經(jīng)元電偶聯(lián)的中間神經(jīng)元。這些不同時(shí)相節(jié)律性發(fā)放的神經(jīng)元有可能是位置移動(dòng)行為蠕動(dòng)波的神經(jīng)基礎(chǔ),而電偶聯(lián)現(xiàn)象可能是時(shí)間節(jié)律性發(fā)放的理論基礎(chǔ)。依據(jù)色素回填的實(shí)驗(yàn),我們猜測(cè)有協(xié)調(diào)型中間神經(jīng)元的存在。我們的研究對(duì)海兔蠕動(dòng)波的中樞控制有了一定初步的了解,為將來(lái)更進(jìn)一步的研究打下了堅(jiān)實(shí)基礎(chǔ)。
[Abstract]:The peristaltic wave rolling wave caused by neural activity is a rhythmic movement widely found in vertebrates and invertebrates. The neural loop that controls this movement is called the central pattern generator. In the sea rabbit Aplysia californica, the flight position movement behavior caused by peristaltic waves is an excellent model for studying the central pattern generator of position movement. The central pattern generator of sea rabbits is located in a single ganglion instead of those that are regulated by multiple ganglion networks or ganglion. Based on previous studies, we focused our work on the ventral ganglion of the foot. We have located a cell mass in the root of the ventral face of the right foot ganglion by pigment backfilling. The semi-isolated experiments have confirmed that they can induce muscle contraction and are further characterized as motoneurons. The electrophysiological recording method was used to record both intracellular and extracellular nerves. It was found that the excitatory mode of motor neurons was different from that of PPCN motor nerves, and the time-dependent relationship between the recorded cells and PPCN was found. The results of intercellular electrocoupling showed that motoneurons with similar phases were electrically coupled with each other. At the same time, the interneuron electrically coupled with a single temporal motor neuron was recorded at the root of p7. These rhythmic neurons may be the neural basis of peristaltic waves, while the electrocoupling phenomenon may be the theoretical basis of temporal rhythmic distribution. Based on the experiment of pigment backfilling, we speculate the existence of coordinated intermediate neurons. Our study provides a preliminary understanding of the central control of peristaltic waves in sea rabbits, which lays a solid foundation for further research.
【學(xué)位授予單位】：南京大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：Q42

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