本文選題：NTP蛋白 + 脅迫��； 參考：《深圳大學》2017年碩士論文

【摘要】：高鹽、干旱、高溫或低溫等非生物脅迫嚴重影響現(xiàn)代農業(yè)的可持續(xù)發(fā)展。而生物本身可以通過調節(jié)RNA的豐度與RNA的穩(wěn)定性來提高對逆境的耐受性。NTP(核苷酸轉移酶,Nucleotidyltransferase)是一類包含Poly(A)聚合酶和NT結構域(末端尿苷酸轉移酶,Nucleotidyltransferase)蛋白,它能在RNA的末端非模板地添加核苷酸,從而改變RNA的穩(wěn)定性。影響RNA在體內的豐度。雖然對植物中個別的NTP蛋白已經(jīng)有初步的研究,但是大多數(shù)NTP蛋白的功能還尚不清楚。本實驗首先通過生物信息學的方法分析了擬南芥中10種NTP的蛋白序列及結構特點,NTP蛋白雖然都擁有相同的結構域的非穩(wěn)定蛋白,但其酸堿性差異較大;NTP蛋白與其他蛋白有明顯的互作關系,并且10種NTP蛋白都與CSDP1直接互作,CSDP1是一種脅迫相關基因,直接參與對脅迫的應答;10種基因中均存在脅迫元件,表明NTP蛋白可能參與各種脅迫應答,并在脅迫響應中發(fā)揮功能。其次本實驗通過熒光定量PCR的方法,對10種NTP基因分別進行了鹽、干旱、冷、熱等脅迫下的誘導表達分析。發(fā)現(xiàn)HESO1、NTP6和NTP7是受鹽、干旱、冷、熱等脅迫誘導。最后通過3種突變體在鹽及干旱中的表型分析,發(fā)現(xiàn)在鹽脅迫下heso1、ntp6和ntp7萌發(fā)率、變綠子葉率、根長及生物量均低于野生型植株(Col-WT),說明heso1、ntp6和ntp7對鹽脅迫的耐受性下降;在干旱脅迫下,heso1、ntp6和ntp7在失水后植物組織受損較Col-WT更嚴重,說明heso1、ntp6和ntp7對干旱脅迫的耐受性也下降。綜上所述,本研究發(fā)現(xiàn)NTP家族中的HESO1、NTP6和NTP7受到非生物脅迫的誘導表達,并對擬南芥的非生物脅迫耐受性產生重要影響,說明HESO1、NTP6和NTP7可能參與植物對環(huán)境脅迫的反應。
[Abstract]:Abiotic stresses such as high salt, drought, high temperature or low temperature seriously affect the sustainable development of modern agriculture.By regulating the abundance of RNA and the stability of RNA, the organism can improve its tolerance to stress. NTP (nucleotidyltransferase) is a class of nucleotidyltransferase and nucleotidyltransferase protein.It can change the stability of RNA by adding nucleotides to the end of RNA without template.The abundance of RNA in vivo was affected.Although a few NTP proteins have been studied in plants, the function of most NTP proteins remains unclear.In this study, the sequence and structural characteristics of 10 NTP proteins in Arabidopsis thaliana were analyzed by bioinformatics.However, there was a significant difference between NTP protein and other proteins, and all 10 NTP proteins were directly interacting with CSDP1. CSDP1 was a stress related gene, and there were stress elements in all 10 genes directly involved in the response to stress.The results suggest that NTP protein may participate in various stress responses and play a role in stress response.Secondly, the induced expression of 10 NTP genes under salt, drought, cold and heat stress were analyzed by fluorescence quantitative PCR.It was found that HESO1 NTP6 and NTP7 were induced by salt, drought, cold and heat stress.Finally, the phenotypic analysis of three mutants in salt and drought showed that the germination rate, green cotyledon rate, root length and biomass of Heso1ntp6 and ntp7 under salt stress were lower than those of wild type plant Col-WTP, which indicated that the tolerance of Hheso1ntp6 and ntp7 to salt stress was decreased.Hesheso1ntp6 and ntp7 were damaged more seriously than Col-WT after water loss, which indicated that the tolerance of Hesheso1ntp6 and ntp7 to drought stress was also decreased.In conclusion, we found that HESO1 NTP6 and NTP7 in NTP family were induced by abiotic stress and had an important effect on abiotic stress tolerance of Arabidopsis thaliana, suggesting that HESO1 NTP6 and NTP7 may be involved in plant response to environmental stress.
【學位授予單位】：深圳大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：Q943.2;Q945.78

【參考文獻】

相關期刊論文 前10條

1 趙彥坤;王秀堂;王靜;傅曉藝;董章輝;史占良;郭進考;何明琦;;熱脅迫對不同小麥品種灌漿速率的影響[J];中國生態(tài)農業(yè)學報;2016年09期

2 李洋;于麗杰;金曉霞;;植物重金屬脅迫耐受機制[J];中國生物工程雜志;2015年09期

3 劉馳;李家寶;芮俊鵬;安家興;李香真;;16S rRNA基因在微生物生態(tài)學中的應用[J];生態(tài)學報;2015年09期

4 朱金方;劉京濤;陸兆華;夏江寶;柳海寧;金悅;;鹽脅迫對中國檉柳幼苗生理特性的影響[J];生態(tài)學報;2015年15期

5 沈恩惠;劉揚;葉楚玉;樊龍江;;植物非編碼小RNA研究進展(英文)[J];浙江大學學報(農業(yè)與生命科學版);2014年04期

6 白冠軍;任衍鋼;宋玉奇;衛(wèi)紅萍;;tRNA的發(fā)現(xiàn)和揭示[J];生物學通報;2014年06期

7 張金政;張起源;孫國峰;何卿;李曉東;劉洪章;;干旱脅迫及復水對玉簪生長和光合作用的影響[J];草業(yè)學報;2014年01期

8 王樹鳳;胡韻雪;孫海菁;施翔;潘紅偉;陳益泰;;鹽脅迫對2種櫟樹苗期生長和根系生長發(fā)育的影響[J];生態(tài)學報;2014年04期

9 楊映雪;;非生物脅迫對植物的影響及植物的抵抗機制[J];生物技術世界;2013年09期

10 饒玉春;楊窯龍;黃李超;潘建偉;馬伯軍;錢前;曾大力;;水稻耐冷脅迫的研究進展[J];分子植物育種;2013年03期

，

本文編號：1750455