長野雷夫松氏菌RE3-4吸附鉛、鎘及對蘆葦?shù)拇偕饔醚芯?/H1>
發(fā)布時間：2018-01-27 06:43
本文關(guān)鍵詞： 菌株RE3-4 促生作用 鉛 鎘 海藻酸鈉小球 出處：《安徽大學(xué)》2017年碩士論文 論文類型：學(xué)位論文
【摘要】：內(nèi)生菌因具有固氮、促生長、抗病害、耐重金屬及降解污染物等特性,通過分泌物質(zhì)促進植物生長,間接達到凈化污染物的目的,且能利用其耐受性和吸附性直接降低環(huán)境中污染物含量。本文以一株蘆葦內(nèi)生菌——長野雷夫松氏菌RE3-4為研究對象,考察了該菌株的生物特性及其應(yīng)用。通過對菌株RE3-4合成生長素及其對重金屬耐受性的研究,揭示其促進蘆葦苗生長的內(nèi)在機制;依據(jù)擬合Langmuir型、Freundlich型等溫吸附曲線,比較菌株RE3-4對鉛、鎘吸附能力的大小;研制具有防流失、可回收的海藻酸鈉(Na-Alg)小球,強化菌株RE3-4在水溶液中去除鉛、鎘離子的能力;采用SEM(-EDX)、FT-IR分析手段,揭示菌株吸附鉛、鎘離子的內(nèi)在機理。主要結(jié)論如下:(1)菌株RE3-4在L-色氨酸(L-trp)存在的條件下能夠合成生長素類似物吲哚乙酸(IAA)。菌株合成的IAA能顯著促進蘆葦苗生長,最佳促生濃度為1.0 mg/LO(2)菌株RE3-4對重金屬離子具有耐受性,其中菌株對鉛的耐受性最強,銅次之,對鎘的耐受性最弱;菌株侵染的蘆葦種子在不同含鉛量培養(yǎng)基上生長不同,且在一定程度(鉛濃度400mg/kg)上菌株能提高蘆葦種子的萌發(fā)率及其幼苗根莖的生長。(3)通過等溫吸附曲線擬合發(fā)現(xiàn)菌株RE3~4可吸附Pb和Cd;在吸附過程中蛋白質(zhì)起主要作用,磷脂起次要作用,多糖、氨基酸等物質(zhì)也對Pb和Cd有吸附作用;菌株通過細胞破損、斷裂、聚集、形變等作用對Pb和Cd離子產(chǎn)生抗性。(4)在水溶液pH為5.0,反應(yīng)運行8 h條件下,Na-Alg小球?qū)b和Cd離子的吸附達到平衡;吸附過程中,O-H、C-H、C-O等官能團與Pb和Cd離子發(fā)生交互作用,多糖區(qū)也吸附了一定量的Pb和Cd;Na-Alg小球表面發(fā)生共價結(jié)合、晶體沉淀及離子交換等反應(yīng)從而減少水溶液中金屬離子的量;吸附Pb和Cd的Na-Alg小球解吸附后可進行二次吸附,具有較好的循環(huán)再利用性能。
[Abstract]:Endophytic bacteria have the characteristics of nitrogen fixation, growth promotion, disease resistance, resistance to heavy metals and degradation of pollutants, through secreting substances to promote plant growth, indirectly achieve the purpose of purifying pollutants. And its tolerance and adsorption can directly reduce the content of pollutants in the environment. In this paper, a Reed endophytic bacterium, RE3-4, was used as the research object. The biological characteristics and application of this strain were investigated. The mechanism of promoting the growth of Reed seedlings was revealed by studying the biosynthesis of auxin and its tolerance to heavy metals by RE3-4. According to fitting the Langmuir type Freundlich isotherm adsorption curve, the adsorption ability of RE3-4 to lead and cadmium was compared. In order to enhance the ability of RE3-4 to remove lead and cadmium ions in aqueous solution, a sodium alginate (Na-Alg) ball with anti-loss and recoverability was developed. It was revealed that the strain adsorbed lead by means of SEMM-EDX FT-IR analysis. The intrinsic mechanism of cadmium ion. The main conclusions are as follows: the strain RE3-4 can synthesize the auxin analogue indoleacetic acid (IIA) in the presence of L-trpL- tryptophan. The IAA synthesized by the strain could significantly promote the growth of Reed seedlings. The best growth promotion concentration was 1.0 mg / L ~ (2)) strain RE3-4 had the highest tolerance to heavy metal ions, among which the strain had the strongest tolerance to lead, followed by copper, and had the weakest tolerance to cadmium. The Reed seeds infected by the strain grew differently on different lead content media. To a certain extent (lead concentration 400 mg / kg), the strain could increase the germination rate of Reed seeds and the growth of seedling rhizomes. The results of isothermal adsorption curve showed that RE3~4 could adsorb Pb and CD. Protein plays a major role in the adsorption process, phospholipid plays a secondary role, polysaccharides, amino acids and other substances also have the adsorption of Pb and CD; The strain produced resistance to Pb and CD ions by cell breakage, breakage, aggregation, deformation and so on. Under the condition of pH 5.0 in aqueous solution, reaction run for 8 h. The adsorption of Pb and CD on Na-Alg beads reached equilibrium. In the process of adsorption, the functional groups such as O-HHU C-HnC-O interact with Pb and CD ions, and some amounts of Pb and CD are also adsorbed in the polysaccharide region. The surface of Na-Alg pellets is covalently bound, crystal precipitation and ion exchange reactions occur so as to reduce the amount of metal ions in aqueous solution. The secondary adsorption of Pb and CD by Na-Alg spheres can be carried out after desorption, and it has good recycling performance.
【學(xué)位授予單位】：安徽大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：X172
【參考文獻】
相關(guān)期刊論文 前10條
1 丁文s，
本文編號：1467857
本文鏈接：http://sikaile.net/shoufeilunwen/benkebiyelunwen/1467857.html
本文關(guān)鍵詞： 菌株RE3-4 促生作用 鉛 鎘 海藻酸鈉小球 出處：《安徽大學(xué)》2017年碩士論文 論文類型：學(xué)位論文
【摘要】：內(nèi)生菌因具有固氮、促生長、抗病害、耐重金屬及降解污染物等特性,通過分泌物質(zhì)促進植物生長,間接達到凈化污染物的目的,且能利用其耐受性和吸附性直接降低環(huán)境中污染物含量。本文以一株蘆葦內(nèi)生菌——長野雷夫松氏菌RE3-4為研究對象,考察了該菌株的生物特性及其應(yīng)用。通過對菌株RE3-4合成生長素及其對重金屬耐受性的研究,揭示其促進蘆葦苗生長的內(nèi)在機制;依據(jù)擬合Langmuir型、Freundlich型等溫吸附曲線,比較菌株RE3-4對鉛、鎘吸附能力的大小;研制具有防流失、可回收的海藻酸鈉(Na-Alg)小球,強化菌株RE3-4在水溶液中去除鉛、鎘離子的能力;采用SEM(-EDX)、FT-IR分析手段,揭示菌株吸附鉛、鎘離子的內(nèi)在機理。主要結(jié)論如下:(1)菌株RE3-4在L-色氨酸(L-trp)存在的條件下能夠合成生長素類似物吲哚乙酸(IAA)。菌株合成的IAA能顯著促進蘆葦苗生長,最佳促生濃度為1.0 mg/LO(2)菌株RE3-4對重金屬離子具有耐受性,其中菌株對鉛的耐受性最強,銅次之,對鎘的耐受性最弱;菌株侵染的蘆葦種子在不同含鉛量培養(yǎng)基上生長不同,且在一定程度(鉛濃度400mg/kg)上菌株能提高蘆葦種子的萌發(fā)率及其幼苗根莖的生長。(3)通過等溫吸附曲線擬合發(fā)現(xiàn)菌株RE3~4可吸附Pb和Cd;在吸附過程中蛋白質(zhì)起主要作用,磷脂起次要作用,多糖、氨基酸等物質(zhì)也對Pb和Cd有吸附作用;菌株通過細胞破損、斷裂、聚集、形變等作用對Pb和Cd離子產(chǎn)生抗性。(4)在水溶液pH為5.0,反應(yīng)運行8 h條件下,Na-Alg小球?qū)b和Cd離子的吸附達到平衡;吸附過程中,O-H、C-H、C-O等官能團與Pb和Cd離子發(fā)生交互作用,多糖區(qū)也吸附了一定量的Pb和Cd;Na-Alg小球表面發(fā)生共價結(jié)合、晶體沉淀及離子交換等反應(yīng)從而減少水溶液中金屬離子的量;吸附Pb和Cd的Na-Alg小球解吸附后可進行二次吸附,具有較好的循環(huán)再利用性能。
[Abstract]:Endophytic bacteria have the characteristics of nitrogen fixation, growth promotion, disease resistance, resistance to heavy metals and degradation of pollutants, through secreting substances to promote plant growth, indirectly achieve the purpose of purifying pollutants. And its tolerance and adsorption can directly reduce the content of pollutants in the environment. In this paper, a Reed endophytic bacterium, RE3-4, was used as the research object. The biological characteristics and application of this strain were investigated. The mechanism of promoting the growth of Reed seedlings was revealed by studying the biosynthesis of auxin and its tolerance to heavy metals by RE3-4. According to fitting the Langmuir type Freundlich isotherm adsorption curve, the adsorption ability of RE3-4 to lead and cadmium was compared. In order to enhance the ability of RE3-4 to remove lead and cadmium ions in aqueous solution, a sodium alginate (Na-Alg) ball with anti-loss and recoverability was developed. It was revealed that the strain adsorbed lead by means of SEMM-EDX FT-IR analysis. The intrinsic mechanism of cadmium ion. The main conclusions are as follows: the strain RE3-4 can synthesize the auxin analogue indoleacetic acid (IIA) in the presence of L-trpL- tryptophan. The IAA synthesized by the strain could significantly promote the growth of Reed seedlings. The best growth promotion concentration was 1.0 mg / L ~ (2)) strain RE3-4 had the highest tolerance to heavy metal ions, among which the strain had the strongest tolerance to lead, followed by copper, and had the weakest tolerance to cadmium. The Reed seeds infected by the strain grew differently on different lead content media. To a certain extent (lead concentration 400 mg / kg), the strain could increase the germination rate of Reed seeds and the growth of seedling rhizomes. The results of isothermal adsorption curve showed that RE3~4 could adsorb Pb and CD. Protein plays a major role in the adsorption process, phospholipid plays a secondary role, polysaccharides, amino acids and other substances also have the adsorption of Pb and CD; The strain produced resistance to Pb and CD ions by cell breakage, breakage, aggregation, deformation and so on. Under the condition of pH 5.0 in aqueous solution, reaction run for 8 h. The adsorption of Pb and CD on Na-Alg beads reached equilibrium. In the process of adsorption, the functional groups such as O-HHU C-HnC-O interact with Pb and CD ions, and some amounts of Pb and CD are also adsorbed in the polysaccharide region. The surface of Na-Alg pellets is covalently bound, crystal precipitation and ion exchange reactions occur so as to reduce the amount of metal ions in aqueous solution. The secondary adsorption of Pb and CD by Na-Alg spheres can be carried out after desorption, and it has good recycling performance.
【學(xué)位授予單位】：安徽大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：X172
【參考文獻】
相關(guān)期刊論文 前10條
1 丁文s，
本文編號：1467857
本文鏈接：http://sikaile.net/shoufeilunwen/benkebiyelunwen/1467857.html
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