本文關(guān)鍵詞：擬南芥生物節(jié)律鐘相關(guān)基因LHY、CCA1和GI對(duì)其營(yíng)養(yǎng)生長(zhǎng)時(shí)相轉(zhuǎn)變的作用　出處：《天津農(nóng)學(xué)院》2017年碩士論文　論文類型：學(xué)位論文

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【摘要】：高等植物的生長(zhǎng)發(fā)育在整個(gè)生命周期中是一個(gè)連續(xù)的過(guò)程,包括胚胎發(fā)育和胚后發(fā)育。胚后發(fā)育也能夠劃分為營(yíng)養(yǎng)發(fā)育期和生殖發(fā)育期,唯有通過(guò)營(yíng)養(yǎng)生長(zhǎng)時(shí)期中的幼齡期和成熟期,才會(huì)完成營(yíng)養(yǎng)生長(zhǎng)時(shí)相轉(zhuǎn)變(vegetative phase change,VPC)。VPC是植物生長(zhǎng)發(fā)育的一個(gè)重要生理過(guò)程,只有經(jīng)歷這一轉(zhuǎn)變,植物體才能進(jìn)入生殖生長(zhǎng),完成開(kāi)花、結(jié)果。植物在生長(zhǎng)發(fā)育過(guò)程中,一些表型變化、莖頂端分生組織的變化以及在分子表達(dá)水平上的不同等,都與營(yíng)養(yǎng)生長(zhǎng)時(shí)相轉(zhuǎn)變有著密不可分的聯(lián)系。最早蓮座葉遠(yuǎn)軸面表皮毛可作為劃分幼齡期和成熟期的一個(gè)標(biāo)記;莖尖分生組織在植株生長(zhǎng)中起著重要作用,是植物生長(zhǎng)發(fā)育的重要途徑;miR156及其靶基因家族SPLs(SQUAMOSA PROMOTER BINDING PROTEIN LIKE)中的SPL3基因在VPC中發(fā)揮重要作用。植物通過(guò)一個(gè)內(nèi)在的“鐘”來(lái)達(dá)到對(duì)夜長(zhǎng)的應(yīng)答進(jìn)行調(diào)控的目的,在一定條件下,植物每天都能維持24 h的節(jié)律,調(diào)控這種節(jié)律的內(nèi)在機(jī)制叫做生物節(jié)律鐘(circadian clock)。營(yíng)養(yǎng)生長(zhǎng)時(shí)相轉(zhuǎn)變與植物自身生物鐘節(jié)律密切相關(guān),但生物節(jié)律鐘相關(guān)基因在此轉(zhuǎn)變中的作用仍不明確。本研究從形態(tài)特點(diǎn)、解剖結(jié)構(gòu)以及調(diào)控VPC的miR156和SPL3表達(dá)的分子檢測(cè)等各方面分析了擬南芥(Arabidopsis thaliana)生物節(jié)律鐘(circadian clock)相關(guān)基因突變體lhy、cca1、gi的營(yíng)養(yǎng)生長(zhǎng)時(shí)相轉(zhuǎn)變特點(diǎn)。結(jié)果表明,突變體lhy、cca1生長(zhǎng)周期與野生型Col-0的相差不大,但gi的生長(zhǎng)周期比Col-0的延長(zhǎng);從蓮座葉葉片的狀態(tài)、葉片長(zhǎng)寬比、遠(yuǎn)軸面表皮毛出現(xiàn)等營(yíng)養(yǎng)生長(zhǎng)形態(tài)特點(diǎn)和莖尖分生組織解剖組織特點(diǎn)剖析,lhy、cca1、gi突變體都延遲了營(yíng)養(yǎng)生長(zhǎng)時(shí)相轉(zhuǎn)變。此外,RT-PCR和Q-PCR檢測(cè)營(yíng)養(yǎng)生長(zhǎng)時(shí)相轉(zhuǎn)變過(guò)程中關(guān)鍵調(diào)控因子miR156和SPL3的表達(dá)量結(jié)果也顯示,lhy、cca1、gi突變體也延遲了營(yíng)養(yǎng)生長(zhǎng)時(shí)相轉(zhuǎn)變。
[Abstract]:Higher plant growth and development throughout the life cycle is a continuous process, including embryo development and embryo development. Post embryonic development can also be divided into nutrition and reproductive growth period and development period, only through the period of vegetative growth in juvenile and mature period, will complete the health camp long phase transition (vegetative phase change, VPC.VPC) is one of the most important physiological processes in plant growth and development, only through this transformation, plants can enter the reproductive growth, flowering, results in plant growth and development, some phenotypic changes, changes in stem apical meristem and in the expression of the different, and vegetative growth when the phase transition is inextricably linked. The earliest rosette leaf abaxial epidermal hairs can be used as a marker of juvenile and mature period; meristem in plants plays an important role in the growth of That is an important way for plant growth and development; miR156 and its target gene family SPLs (SQUAMOSA PROMOTER BINDING PROTEIN LIKE) in the SPL3 gene play an important role in VPC. The plant through an internal clock to achieve the regulation of the night long response, under certain conditions, the plant every day maintain 24 h rhythm, the intrinsic mechanism of regulation of this rhythm is called circadian clock (circadian clock). The vegetative growth phase transition is closely related with the plant's own biological clock rhythm, but the change of circadian clock related genes in this role is not clear. This study from the aspects of morphological characteristics, anatomical structure and expression regulation VPC miR156 and SPL3 detection analysis of Arabidopsis thaliana (Arabidopsis thaliana) biological clock (circadian clock) gene mutant lhy, CCA1, GI during the vegetative growth phase transition characteristics. Results Ming, mutant lhy, CCA1 growth cycle has little difference with the wild type Col-0, but the growth cycle of GI than the Col-0 extension; from rosette leaf state, leaf length and width ratio, abaxial trichomes appeared as vegetative morphological features and meristem anatomical characteristics analysis, lhy, CCA1, GI mutants have delayed the vegetative growth phase transition. In addition, the expression of RT-PCR and Q-PCR detection when the vegetative growth phase transformation of key regulatory factor miR156 and SPL3 results also showed that lhy, CCA1, GI mutants also delayed the vegetative growth phase shift.

【學(xué)位授予單位】：天津農(nóng)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：Q943.2;Q945

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本文編號(hào)：1406949