吲哚類化合物引起的環(huán)境污染日益嚴(yán)重。在動物養(yǎng)殖中,來源于L-色氨酸無氧代謝產(chǎn)生的糞臭素（3-甲基吲哚,3MI）等吲哚類化合物是養(yǎng)殖場臭氣的主要成分,也是造成養(yǎng)殖場及其周邊環(huán)境惡化的主要因素。如何有效治理吲哚污染是養(yǎng)殖業(yè)可持續(xù)發(fā)展所面臨的難題。本研究使用富集培養(yǎng)法從家禽糞便中分離出能夠利3MI和吲哚的可培養(yǎng)細菌,在進行生化和分子鑒定的基礎(chǔ)上研究了其生化特性、培養(yǎng)條件、除臭功能及其機理,為吲哚生物除臭飼料添加劑的研制奠定基礎(chǔ)。本研究分離獲得能夠高效降解糞臭素的細菌2株,通過使用Biolog GEN III MicroPlate評估所分離菌株的生化特征并依據(jù)16S rDNA基因測序結(jié)果,將其鑒定為不動桿菌NTA1-2A（A.Toweneri）和不動桿菌TAT1-6A（A.guillouiae）。通過單因素實驗確定了降解糞臭素（3MI）、吲哚的最佳溫度和pH值,評估對糞臭素和吲哚的初始濃度的耐受性以及初始濃度對降解效果的影響。結(jié)果表明,菌株NTA1-2A和TAT1-6A降解3MI的最佳溫度和pH分別為31℃和6,兩菌株可有效降解初始濃度范圍為65²00mg/L的3MI;當(dāng)... 

【文章來源】：中國農(nóng)業(yè)科學(xué)院北京市

【文章頁數(shù)】：107 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
abstract
LIST OF ACRONYMS
CHAPTER1 INTRODUCTION
    1.1 GENERAL INTRODUCTION
CHAPTER2 LITERATURE REVIEW
    2.1 STUDY BACKGROUND
    2.2 FORMATION OF INDOLE AND SKATOLE
    2.3 3-METHYLINDOLE（SKATOLE）
        2.3.1 Effects of3-methylindole
        2.3.2 Bacterial degradation of3-methylindole
    2.4 MICROBIAL DEGRADATION OF INDOLE
        2.4.1 Anaerobic bacteria degradation of indole
        2.4.2 Aerobic degradation of indole
        2.4.3 Bacterial cometabolism of indole
        2.4.4 Fungal degradation of indole
    2.5 THE CURRENT STUDY
        2.5.1 Aims and objectives of current study
        2.5.2 Purpose and significance of the study
CHAPTER3 ISOLATION AND MOLECULAR CHARACTERIZATION OF3-METHYLINDOLEDEGRADING BACTERIA FROM POULTRY MANURE
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 Bacteria growth medium
        3.2.2 Identification of the selected strains NTA1-2A and TAT-6A
        3.2.3 Assessing3MI degradation
        3.2.4 Single factors affecting3MI degradation
        3.2.5 Effect of3MI initial concentrations
        3.2.6 Enzyme activity test
        3.2.7 Poultry manure fermentation experiment
        3.2.8 Sample preparation for HPLC analysis
    3.3 STATISTICAL ANALYSIS
    3.4 RESULTS
        3.4.1 Identification of strains NTA1-2A and TAT1-6A
        3.4.2 Effect of temperature and pH on growth and3MI Degradation
        3.4.3 3MI degradation at different initial concentrations
        3.4.4 3MI degradation efficiency of supernatant
        3.4.5 3MI removal from poultry manure
    3.5 DISCUSSION
    3.6 CONCLUSION
CHAPTER4 DEGRADATION OF INDOLE USING A.TOWENERI NTA1-2A AND A.GUILLOUIAE TAT1-6A BACTERIAL STRAINS
    4.1 INTRODUCTION
    4.2 MATERIALS AND METHODS
        4.2.1 Source of microorganisms
        4.2.2 Medium conditions and bacteria growth
        4.2.3 Indole degradation by NTA1-2A and TAT1-6A
        4.2.4 Factors affecting Indole degradation
        4.2.5 Enzyme activity test
        4.2.6 Poultry manure fermentation
        4.2.7 Sample preparation for HPLC analysis
    4.3 STATISTICAL ANALYSIS
    4.4 RESULTS
        4.4.1 Indole degradation efficiency
        4.4.2 Indole degradation at different initial concentrations
        4.4.3 Factors affecting Indole degradation
        4.4.4 Effect of indole on bacterial growth
        4.4.5 Indole degradation efficiency of supernatant
        4.4.6 Indole removal from poultry manure
    4.5 DISCUSSION
    4.6 CONCLUSION
CHAPTER5 ANALYSIS OF3-METHYLINDOLE AND INDOLE DEGRADATIONMETABOLITES
    5.1 INTRODUCTION
        5.1.1 3-methylindole degradation metabolites
        5.1.2 Indole degradation metabolites
    5.2 MATERIALS AND METHODS
        5.2.1 Source of microorganisms and Medium conditions
        5.2.2 Sample preparation and LC– MS/MS analysis
        5.2.3 Data analysis
        5.2.4 Protein identification by SDS-PAGE
    5.3 RESULTS
        5.3.1 3-methylindole degradation metabolites
        5.3.2 Indole degradation metabolites
        5.3.3 Metabolic enzymes
    5.4 DISCUSSION
    5.5 CONCLUSIONS
CHAPTER6 OVERALL CONCLUSIONS AND RECOMMENDATIONS
    6.1 CONCLUSIONS
    6.2 RECOMMENDATIONS
CHAPTER7 REFERENCES
ACKNOWLEDGMENTS
AUTHOR RESUME
APPENDICES



本文編號：3105317