【摘要】：國(guó)內(nèi)外對(duì)細(xì)菌的作用及作用機(jī)制研究較多,而對(duì)真菌的作用及機(jī)制研究較少.為了提高NO,NO_x的脫除效率,本文對(duì)脫氮塔生物膜中的反硝化真菌進(jìn)行了分離、純化及鑒定,通過搖瓶實(shí)驗(yàn)研究了反硝化真菌對(duì)NO_3~-的作用特征,最后在脫氮塔中投加了分離的反硝化真菌純菌株擴(kuò)大培養(yǎng)液,研究了其對(duì)NO_x脫除效率的影響,還對(duì)脫氮塔內(nèi)真菌的群落結(jié)構(gòu)進(jìn)行了高通量測(cè)序分析.實(shí)驗(yàn)結(jié)果表明:經(jīng)過真菌形態(tài)特征和脫氮(NO_3~-)特性研究及ITS序列測(cè)定分析,將3LNB菌株鑒定為鐮刀菌的腐皮鐮刀菌Fusarium solani.高通量測(cè)序分析結(jié)果證明了脫氮塔內(nèi)確實(shí)存在鐮刀菌屬的腐皮鐮刀菌Fusarium solani.在NO_3~-—N濃度高達(dá)10.01g/L的條件下,實(shí)驗(yàn)共10d,細(xì)菌反硝化組反硝化率為5.6%,其脫除效率為0.56g/L;真菌3LNB菌株的反硝化率能達(dá)到15.1%,其脫除效率為1.51g/L.實(shí)驗(yàn)室實(shí)驗(yàn)證明3LNB菌株對(duì)NO_3~-具有較強(qiáng)的反硝化能力.將3LNB純菌株擴(kuò)大培養(yǎng)液400mL(4%)投加到脫氮塔中,NO,NO_x的平均脫除率分別提高了5.12%,5.36%.脫氮塔凈化實(shí)驗(yàn)證明3LNB培養(yǎng)液能強(qiáng)化微生物對(duì)NO,NO_x的脫除效能,這可能主要與腐皮鐮刀菌等真菌細(xì)胞存在細(xì)胞色素P450nor有關(guān).
[Abstract]:There are many studies on the function and mechanism of bacteria at home and abroad, but less on fungi. In order to improve the removal efficiency of NO,NO_x, denitrifying fungi in biofilm of denitrification tower were isolated, purified and identified, and the action characteristics of denitrifying fungi on NO_3~- were studied by shaking flask experiment. Finally, the expanded culture medium of isolated denitrifying fungi was added to the denitrifying tower, and the effect of the isolated denitrifying fungi on the removal efficiency of NO_x was studied, and the community structure of fungi in the denitrification tower was analyzed by high throughput sequencing. The results showed that the 3LNB strain was identified as Fusarium saprophyllum Fusarium solani. by studying the morphological characteristics of fungi, the characteristics of NO_3~- and the analysis of ITS sequence. The results showed that the strain of Fusarium oxysporum was identified as Fusarium oxysporum Fusarium solani.. The results of high throughput sequencing showed that there was a Fusarium solani. in the denitrifying tower. When the concentration of NO_3~--N was as high as 10.01g/L, the denitrification rate of bacterial denitrification group was 5.6% and the removal efficiency was 0.56 g / L for 10 days. The denitrification rate of fungal 3LNB strain was up to 15.1%, and the removal efficiency was 1.51g 路L ~ (- 1) 路h ~ (- 1). Laboratory experiments showed that 3LNB strain had strong denitrification ability to NO_3~-. The average removal rate of 3LNB was increased by 5.12% and 5.36% respectively when 400mL (4%) was added to the nitrogen removal column. The purification experiment of nitrogen removal column showed that 3LNB medium could enhance the removal efficiency of NO,NO_x by microorganisms, which might be related to the existence of cytochrome P450nor in fungal cells, such as Fusarium saprophyllum.
【作者單位】： 云南大學(xué)生態(tài)學(xué)與環(huán)境學(xué)院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(51168046,51278447) 云南省自然科學(xué)基金資助項(xiàng)目(2015FB111)~~
【分類號(hào)】：X172;X701

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本文編號(hào)：2473812