發(fā)布時(shí)間：2019-01-02 09:22

【摘要】：為系統(tǒng)考察磺胺類抗生素藥物(SAs)污染物長(zhǎng)期暴露下的生物毒性效應(yīng),以5種磺胺類抗生素(SAs):磺胺氯噠嗪(SCP)、磺胺嘧啶(SD)、磺胺吡啶(SPY)、磺胺甲惡唑(SMX)和磺胺二甲嘧啶(SM2)為研究對(duì)象,應(yīng)用直接均分射線法(Equ-Ray)設(shè)計(jì)10個(gè)二元抗生素混合物體系(每個(gè)二元混合物體系設(shè)計(jì)5條具有不同濃度比的射線),應(yīng)用優(yōu)化的長(zhǎng)期微板毒性分析法(L-MTA)系統(tǒng)測(cè)試這些抗生素在16 h對(duì)發(fā)光菌青海弧菌(Vibrio-qinghaiensis sp.-Q67,Q67)的發(fā)光抑制毒性,并應(yīng)用濃度加和模型(CA)分析混合物毒性相互作用。結(jié)果表明:5種SAs及其混合物射線對(duì)Q67在16 h呈現(xiàn)明顯的毒性,但不同的抗生素毒性大小不同,以半數(shù)效應(yīng)濃度的負(fù)對(duì)數(shù)(pEC_(50))為毒性大小指標(biāo),5種SAs的毒性大小順序?yàn)?SMX(p EC50=3.95)SCP(p EC50=3.65)SPY(pEC_(50)=3.41)SD(pEC_(50)=3.36)SM2(pEC_(50)=3.21);10個(gè)SAs的二元混合物體系中7個(gè)呈現(xiàn)出加和作用,3個(gè)呈現(xiàn)出拮抗作用;3個(gè)混合物體系拮抗作用隨組分濃度比的變化呈現(xiàn)不同的變化規(guī)律:混合物體系SCP-SPY和SCP-SMX中的射線拮抗作用均隨SCP濃度比逐漸減小,即從R1到R2逐漸明顯,從R2到R5幾乎不變,而SCP-SM2體系,拮抗作用隨組分SCP的濃度比逐漸減小,即從R1到R2逐漸明顯,從R2到R5,逐漸變得不明顯,R5在較高濃度區(qū)甚至出現(xiàn)了協(xié)同作用。
[Abstract]:In order to investigate the biological toxicity of sulfamethylamine antibiotic (SAs) under long-term exposure, five kinds of sulfonamides, (SAs):, sulfadiazine, (SCP), sulfadiazine, (SD), sulfamyridine, (SPY), were used in this study. Sulfamethoxazole (SMX) and sulfamethazine (SM2) were studied. Ten binary antibiotic mixtures (5 rays with different concentration ratios were designed for each binary mixture system) were designed by direct mean ray method (Equ-Ray). The chemiluminescence inhibition toxicity of these antibiotics to Vibrio Qinghai (Vibrio-qinghaiensis sp.-Q67,Q67) at 16 h was tested by an optimized long-term microplate toxicity analysis (L-MTA) system. The toxicity interaction of mixture was analyzed by concentration sum model (CA). The results showed that five kinds of SAs and its mixture showed obvious toxicity to Q67 at 16 h, but the toxicity of different antibiotics was different. The negative logarithm of half effect concentration (pEC_ (50) was taken as the index of toxicity. The order of toxicity of five SAs was: SMX (p EC50=3.95) SCP (p EC50=3.65) SPY (pEC_ (50) = 3.41) SD (pEC_ (50) = 3.36 SM2 (pEC_ (50) = 3.21). Of the 10 binary mixture systems of SAs, 7 showed additive action and 3 showed antagonism. The antagonism of the three mixture systems showed different laws with the change of component concentration ratio: the ray antagonism in SCP-SPY and SCP-SMX decreased with the SCP concentration ratio, that is, from R1 to R2, the antagonism was obvious from R1 to R2. From R2 to R5, the antagonism decreased with the concentration ratio of SCP, that is, from R1 to R2, but from R2 to R5, and the antagonism of R5 even showed synergism in the higher concentration of R5.
【作者單位】： 安徽建筑大學(xué)環(huán)境與能源工程學(xué)院安徽省水污染控制與廢水資源化重點(diǎn)實(shí)驗(yàn)室;清華大學(xué)新興有機(jī)污染物控制北京市重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(21677001,21207002) 安徽省自然科學(xué)基金項(xiàng)目(1708085MB50)~~
【分類號(hào)】：X171.5

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