【摘要】：探討超富集植物高濃度金屬富集量對其生理機制的影響,對于了解超富集植物強大的解毒機制與其生理機制的相關(guān)性有重要意義。采用室內(nèi)原礦土栽培的方法,研究了賀州某錳礦區(qū)未開采區(qū)(T2)、探礦區(qū)(T3)、恢復區(qū)(T4)、開采區(qū)(T5)、尾礦庫(T6)土壤及桂林未受污染的菜園土(T1)對錳超富集植物酸模葉蓼(Polygonum lapathifolium L.)生長,錳吸收,葉片銨態(tài)氮、硝態(tài)氮、游離脯氨酸、可溶性蛋白質(zhì)含量及氮素代謝關(guān)鍵酶(硝酸還原酶NR)、谷氨酰胺合成酶GS、谷氨酸合酶GOGAT和谷氨酸脫氫酶GDH)活性的影響。結(jié)果表明,隨著Mn處理濃度的提高,酸模葉蓼根、莖、葉中Mn含量顯著增加(P0.05),葉綠素a、葉綠素b和總?cè)~綠素在T3處理時含量最高,此時分別為對照的1.15、1.23和1.17倍,但所有錳處理下酸模葉蓼生物量無顯著性差異(P0.05)。Mn處理一定程度上提高了葉片中可溶性蛋白質(zhì)含量,引起了酸模葉蓼氮素代謝關(guān)鍵酶活性的變化及硝態(tài)氮、銨態(tài)氮和脯氨酸含量的顯著增加(P0.05)。T6處理硝態(tài)氮、銨態(tài)氮和脯氨酸含量最高,分別比對照提高了17.6%、43.8%和206.2%。NR、GS和GOGAT活性呈先增加后降低的變化趨勢,NR、GS和GOGAT活性分別在T2、T3、T4處理時最高,此時分別比對照提高了6.4%、28.8%和66.4%。GDH活性則隨著Mn處理濃度的增加而增加,在T6處理時活性最高,比對照提高了1.74倍。以上表明,GDH在解毒Mn毒害過程中具有重要作用。
[Abstract]:It is important to explore the effect of high concentration metal enrichment on the physiological mechanism of superenriched plants, and to understand the correlation between the strong detoxification mechanism and physiological mechanism of super-enriched plants. The effects of unmined (T2), prospecting (T3), recovery (T4), mining area (T5), tailings (T6) and uncontaminated vegetable garden soil (T1) on manganese superenriched (Polygonum lapathifolium L.) were studied in a manganese mine area in Hezhou. Growth, manganese uptake, leaf ammonium nitrogen, nitrate nitrogen, free proline, soluble protein content and activities of key enzymes of nitrogen metabolism (nitrate reductase NR), glutamine synthase GS, glutamate synthase GOGAT and glutamate dehydrogenase GDH) were investigated. The results showed that the content of Mn in the root, stem and leaf of Polygonum hydropiper increased significantly with the increase of Mn concentration (P0.05). The content of chlorophyll a, chlorophyll b and total chlorophyll in the treatment of T3 was the highest, which was 1.150.23 and 1.17 times of that of the control, respectively. However, there was no significant difference in the biomass of Polygonum acuminatum under all manganese treatments (P0.05). Mn treatment increased the content of soluble protein in leaves to some extent, and caused the change of key enzyme activity of nitrogen metabolism and nitrate nitrogen in leaves of Polygonum hydropiper L.). The content of ammonium nitrogen and proline increased significantly (P0.05). Ammonium nitrogen and proline contents were the highest in T6 treatment, which increased by 17.6% and 206.2% respectively compared with the control. The activities of NR,GS and GOGAT increased first and then decreased. The activities of NR,GS and GOGAT were the highest in T2T3T4 treatment. Compared with the control, the activity of 66.4%.GDH and the activity of 66.4%.GDH were increased by 6.48% and 28.8%, respectively, and increased with the increase of Mn concentration. The activity of T6 was the highest and 1.74 times higher than that of the control. These results suggest that GDH plays an important role in the detoxification of Mn.
【作者單位】： 邵陽學院城鄉(xiāng)建設學院;廣西師范大學生命科學學院;廣西師范大學環(huán)境與資源學院;廣西壯族自治區(qū)環(huán)境監(jiān)測中心站;桂林電子科技大學生命與環(huán)境科學學院;
【基金】：國家自然科學基金項目(41661077;51368011) 國家重點研發(fā)計劃項目(2017YFD0801500) 湖南省自然科學基金項目(2016JJ6135) 廣西自然科學基金項目(2016GXNSFAA380046) 桂林市科學研究與技術(shù)開發(fā)項目(2016012504) 廣西巖溶生態(tài)與環(huán)境變化研究重點實驗室項目(YRHJ15K002;YRHJ15Z026)
【分類號】：X173;X53

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