【摘要】：探討超富集植物高濃度金屬富集量對(duì)其生理機(jī)制的影響,對(duì)于了解超富集植物強(qiáng)大的解毒機(jī)制與其生理機(jī)制的相關(guān)性有重要意義。采用室內(nèi)原礦土栽培的方法,研究了賀州某錳礦區(qū)未開(kāi)采區(qū)(T2)、探礦區(qū)(T3)、恢復(fù)區(qū)(T4)、開(kāi)采區(qū)(T5)、尾礦庫(kù)(T6)土壤及桂林未受污染的菜園土(T1)對(duì)錳超富集植物酸模葉蓼(Polygonum lapathifolium L.)生長(zhǎng),錳吸收,葉片銨態(tài)氮、硝態(tài)氮、游離脯氨酸、可溶性蛋白質(zhì)含量及氮素代謝關(guān)鍵酶(硝酸還原酶NR)、谷氨酰胺合成酶GS、谷氨酸合酶GOGAT和谷氨酸脫氫酶GDH)活性的影響。結(jié)果表明,隨著Mn處理濃度的提高,酸模葉蓼根、莖、葉中Mn含量顯著增加(P0.05),葉綠素a、葉綠素b和總?cè)~綠素在T3處理時(shí)含量最高,此時(shí)分別為對(duì)照的1.15、1.23和1.17倍,但所有錳處理下酸模葉蓼生物量無(wú)顯著性差異(P0.05)。Mn處理一定程度上提高了葉片中可溶性蛋白質(zhì)含量,引起了酸模葉蓼氮素代謝關(guān)鍵酶活性的變化及硝態(tài)氮、銨態(tài)氮和脯氨酸含量的顯著增加(P0.05)。T6處理硝態(tài)氮、銨態(tài)氮和脯氨酸含量最高,分別比對(duì)照提高了17.6%、43.8%和206.2%。NR、GS和GOGAT活性呈先增加后降低的變化趨勢(shì),NR、GS和GOGAT活性分別在T2、T3、T4處理時(shí)最高,此時(shí)分別比對(duì)照提高了6.4%、28.8%和66.4%。GDH活性則隨著Mn處理濃度的增加而增加,在T6處理時(shí)活性最高,比對(duì)照提高了1.74倍。以上表明,GDH在解毒Mn毒害過(guò)程中具有重要作用。
[Abstract]:It is important to explore the effect of high concentration metal enrichment on the physiological mechanism of superenriched plants, and to understand the correlation between the strong detoxification mechanism and physiological mechanism of super-enriched plants. The effects of unmined (T2), prospecting (T3), recovery (T4), mining area (T5), tailings (T6) and uncontaminated vegetable garden soil (T1) on manganese superenriched (Polygonum lapathifolium L.) were studied in a manganese mine area in Hezhou. Growth, manganese uptake, leaf ammonium nitrogen, nitrate nitrogen, free proline, soluble protein content and activities of key enzymes of nitrogen metabolism (nitrate reductase NR), glutamine synthase GS, glutamate synthase GOGAT and glutamate dehydrogenase GDH) were investigated. The results showed that the content of Mn in the root, stem and leaf of Polygonum hydropiper increased significantly with the increase of Mn concentration (P0.05). The content of chlorophyll a, chlorophyll b and total chlorophyll in the treatment of T3 was the highest, which was 1.150.23 and 1.17 times of that of the control, respectively. However, there was no significant difference in the biomass of Polygonum acuminatum under all manganese treatments (P0.05). Mn treatment increased the content of soluble protein in leaves to some extent, and caused the change of key enzyme activity of nitrogen metabolism and nitrate nitrogen in leaves of Polygonum hydropiper L.). The content of ammonium nitrogen and proline increased significantly (P0.05). Ammonium nitrogen and proline contents were the highest in T6 treatment, which increased by 17.6% and 206.2% respectively compared with the control. The activities of NR,GS and GOGAT increased first and then decreased. The activities of NR,GS and GOGAT were the highest in T2T3T4 treatment. Compared with the control, the activity of 66.4%.GDH and the activity of 66.4%.GDH were increased by 6.48% and 28.8%, respectively, and increased with the increase of Mn concentration. The activity of T6 was the highest and 1.74 times higher than that of the control. These results suggest that GDH plays an important role in the detoxification of Mn.
【作者單位】： 邵陽(yáng)學(xué)院城鄉(xiāng)建設(shè)學(xué)院;廣西師范大學(xué)生命科學(xué)學(xué)院;廣西師范大學(xué)環(huán)境與資源學(xué)院;廣西壯族自治區(qū)環(huán)境監(jiān)測(cè)中心站;桂林電子科技大學(xué)生命與環(huán)境科學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(41661077;51368011) 國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2017YFD0801500) 湖南省自然科學(xué)基金項(xiàng)目(2016JJ6135) 廣西自然科學(xué)基金項(xiàng)目(2016GXNSFAA380046) 桂林市科學(xué)研究與技術(shù)開(kāi)發(fā)項(xiàng)目(2016012504) 廣西巖溶生態(tài)與環(huán)境變化研究重點(diǎn)實(shí)驗(yàn)室項(xiàng)目(YRHJ15K002;YRHJ15Z026)
【分類(lèi)號(hào)】：X173;X53

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