發(fā)布時(shí)間：2018-06-05 21:50

  本文選題：瞿麥 + AM真菌　； 參考：《河北大學(xué)》2017年碩士論文

【摘要】：近年來,中藥材瞿麥(Dianthus superbus L.)的抗腫瘤作用受到普遍關(guān)注,其需求量逐漸增大。但瞿麥的道地產(chǎn)區(qū)河北、內(nèi)蒙古等地的土壤均有不同程度的鉛含量超標(biāo)問題,使鉛在種植的藥材中累積,進(jìn)一步影響臨床醫(yī)治效果。目前,國(guó)內(nèi)外常用化學(xué)、生物共生、聯(lián)合共生等方法來進(jìn)行土壤修復(fù),但對(duì)于是否會(huì)影響種植植物的品質(zhì)卻鮮有報(bào)道。本文通過盆栽實(shí)驗(yàn)研究了接種叢枝菌根真菌(AM真菌)、種植共生植物(三葉草)、分別添加重金屬螯合物EDTA和磷肥磷酸鈣對(duì)鉛污染下瞿麥生長(zhǎng)、品質(zhì)及藥效的影響,為科學(xué)種植中藥材提供實(shí)踐依據(jù)。主要研究結(jié)果如下:1.除添加EDTA外,其他方法均能阻礙瞿麥對(duì)土壤中鉛的吸收,使鉛含量顯著降低,在AM真菌、三葉草與瞿麥共生時(shí),瞿麥中的鉛含量達(dá)到最低,降至1.29 mg·g-1。與對(duì)照組相比,有AM真菌參與的修復(fù)技術(shù)對(duì)瞿麥生長(zhǎng)的改善最為顯著。2.運(yùn)用響應(yīng)面法優(yōu)化了瞿麥中大黃素的提取條件,最終確定了瞿麥中大黃素提取的最優(yōu)條件為:液固比61倍,加入1 mL質(zhì)量分?jǐn)?shù)為8%的鹽酸,在40℃時(shí)超聲30 min。并測(cè)得在與AM真菌共生時(shí),瞿麥中活性成分累計(jì)最多,大黃素含量為6.5 mg·g~(-1)。3.使用MTT法測(cè)定瞿麥的石油醚提取物對(duì)子宮頸癌細(xì)胞系Hela細(xì)胞和人胃癌細(xì)胞株MGC-803的抑制作用。實(shí)驗(yàn)表明,瞿麥?zhǔn)兔烟崛∥飳?duì)Hela細(xì)胞的抑制率更顯著(EDTA組除外),且植物體內(nèi)重金屬的含量與腫瘤細(xì)胞抑制率也有一定的相關(guān)性。
[Abstract]:In recent years, Dianthus superbus L. The anti-tumor effect of 1% has received widespread attention, and its demand is increasing gradually. However, the lead content in the soils of Hebei, Inner Mongolia and other places in the true-producing areas of Qumai was higher than the standard, which caused the accumulation of lead in the cultivated medicinal materials and further affected the clinical therapeutic effect. At present, chemical, biological symbiosis, combined symbiosis and other methods are commonly used to repair soil at home and abroad, but there are few reports on whether it will affect the quality of planting plants. The effects of inoculation of arbuscular mycorrhizal fungi (Amycorrhizal mycorrhizal fungi) on the growth, quality and efficacy of diqu wheat under lead pollution were studied in this paper. The effects of chelate EDTA and calcium phosphate fertilizer on the growth, quality and efficacy of diqu wheat under lead pollution were studied in this paper. To provide practical basis for scientific cultivation of traditional Chinese medicine. The main results are as follows: 1. In addition to the addition of EDTA, all the other methods could inhibit the absorption of lead in soil by diqu wheat, and make the lead content decrease significantly. When AM fungi, clover and cu wheat symbiosis, the lead content in diqu wheat reached the lowest level, to 1.29 mg g -1. Compared with the control group, the improvement of diqu wheat growth with AM fungi was the most significant. 2. 2. The extraction conditions of emodin from diqu wheat were optimized by response surface method. The optimum extraction conditions were determined as follows: the ratio of liquid to solid was 61 times, the concentration of 1 mL hydrochloric acid was 8%, and the ultrasonic time was 30 min at 40 鈩，

本文編號(hào)：1983515