本文選題：污水處理 + 細(xì)胞態(tài)DNA�。� 參考：《環(huán)境科學(xué)》2017年09期

【摘要】：為探究污水處理系統(tǒng)中抗生素抗性基因(ARGs)、特別是胞外游離態(tài)ARGs的賦存特征,本研究選取生活污水處理系統(tǒng)和工業(yè)廢水處理系統(tǒng)各一座,采用熒光定量PCR對(duì)細(xì)胞態(tài)及游離態(tài)ARGs豐度變化開(kāi)展研究.在生活污水處理系統(tǒng)M中,進(jìn)水sulⅡ、tet C、bla PSE-1和erm B這4種ARGs細(xì)胞態(tài)的絕對(duì)豐度均大幅高于游離態(tài)的絕對(duì)豐度,生物處理未對(duì)抗生素抗性菌(ARBs)產(chǎn)生富集效應(yīng);MBR的超濾膜有效削減了水中細(xì)胞態(tài)和游離態(tài)DNA,最終ARGs的總?cè)コ蕿?.54~4.95 logs.在焦化廢水處理系統(tǒng)C中,生物處理對(duì)攜帶sulⅡ的ARBs產(chǎn)生了富集效應(yīng),但游離態(tài)sulⅡ的相對(duì)豐度和絕對(duì)豐度均有所降低;其后混凝-砂濾工藝使水中細(xì)胞態(tài)和游離態(tài)sulⅡ的絕對(duì)豐度分別出現(xiàn)了下降和上升,游離態(tài)sulⅡ在總sulⅡ中的比例從生物處理出水中的0.05%,上升到混凝-砂濾出水中的1.33%,并在25℃恒溫避光靜置5d后進(jìn)一步上升至9.31%.ARBs深度去除及殘留細(xì)胞裂解,使污水處理系統(tǒng)出水中游離ARGs在總ARGs中的比例有所上升.游離態(tài)ARGs介導(dǎo)ARGs在污水處理系統(tǒng)出水受納環(huán)境中的傳播擴(kuò)散風(fēng)險(xiǎn)有待后續(xù)研究進(jìn)行深入評(píng)估.
[Abstract]:In order to investigate the occurrence characteristics of antibiotic resistant gene ARGsN, especially extracellular free ARGs in sewage treatment system, a domestic wastewater treatment system and an industrial wastewater treatment system were selected in this study. The changes of cellular and free ARGs abundance were studied by fluorescence quantitative PCR. In the domestic sewage treatment system M, the absolute abundance of four ARGs cell forms, sul 鈪，

本文編號(hào)：1960971