本文選題：高效液相色譜 + 金屬有機骨架　； 參考：《曲阜師范大學(xué)》2017年碩士論文

【摘要】：長期以來,食品和環(huán)境污染物的存在對人類的健康有著巨大的威脅,其來源廣泛,成分復(fù)雜多樣。長期暴露于這些污染物,即使含量較低,也可能經(jīng)過人體長期蓄積而引起細(xì)胞及組織代謝異常,造成人體免疫功能下降,從而導(dǎo)致各種機能障礙,還有可能誘發(fā)腫瘤。因此,開發(fā)高效、靈敏的檢測方法對食品和環(huán)境污染物進(jìn)行快速檢測,對于保護(hù)環(huán)境以及維護(hù)人類健康顯得十分重要。為了得到更加準(zhǔn)確的分析測定結(jié)果,進(jìn)行儀器分析之前,通常需要選擇適當(dāng)?shù)臉悠非疤幚砑夹g(shù),對痕量組分進(jìn)行萃取分離富集。本文主要研究了幾種新型樣品前處理方法在污染物萃取方面的應(yīng)用,并結(jié)合高效液相色譜對幾類食品及環(huán)境污染物進(jìn)行了檢測。主要研究內(nèi)容如下:第一章:綜述了高效液相色譜技術(shù)以及金屬有機骨架材料的特點,合成方法及相關(guān)應(yīng)用。第二章:采用分散液液微萃取對氯代苯氧酸除草劑進(jìn)行萃取,并用2-(11H-苯并[a]咔唑-11-基)-乙基-4-甲基苯磺酸(BCETS)衍生試劑對待測物進(jìn)行標(biāo)記,結(jié)合HPLC-FLD對其進(jìn)行檢測。通過對萃取條件及衍生條件的優(yōu)化,該方法成功應(yīng)用于農(nóng)作物中8種氯代苯氧酸類除草劑的檢測。第三章:成功合成了UiO-67金屬有機骨架材料,將其作為分散固相萃取吸附劑對幾種植物生長調(diào)節(jié)劑進(jìn)行萃取富集,并用熒光衍生試劑對待測物進(jìn)行標(biāo)記,通過質(zhì)譜鑒定解釋了該類衍生反應(yīng)的原理,通過對衍生條件以及萃取條件的優(yōu)化,實現(xiàn)了水果中植物生長調(diào)節(jié)劑的高靈敏檢測。第四章:成功合成了具有大尺寸孔徑的JUC-48金屬有機骨架材料,通過XRD,掃描電子顯微鏡鑒定了其結(jié)構(gòu)。利用材料的大尺寸孔徑,高比面積的特點將其應(yīng)用于多環(huán)芳烴的富集,結(jié)合高效液相色譜分析,建立了多環(huán)芳烴的靈敏檢測方法,在最優(yōu)條件下,該方法成功檢測了環(huán)境水樣中7種多環(huán)芳烴。第五章:成功地合成了磁性固相微萃取吸附劑Fe_3O_4@JUC-48磁性復(fù)合材料,并將其用于磺胺類抗生素的磁固相萃取,該方法操作簡單,消耗有機溶劑少,萃取效率高,聯(lián)合高效液相色譜檢測,實現(xiàn)了肉類食品中的6種磺胺類抗生素的高靈敏檢測。
[Abstract]:For a long time, the existence of food and environmental pollutants has a great threat to human health. The long-term exposure to these pollutants, even if the content is low, may lead to abnormal metabolism of cells and tissues through long-term accumulation of human body, resulting in the decline of human immune function, resulting in various functional disorders, and may induce tumors. Therefore, it is very important to develop an efficient and sensitive method for the rapid detection of food and environmental pollutants for the protection of the environment and the maintenance of human health. In order to obtain more accurate analytical results, it is usually necessary to select appropriate sample pretreatment techniques for the extraction and enrichment of trace components before instrument analysis. In this paper, the application of several new sample pretreatment methods in pollutant extraction was studied, and several kinds of food and environmental pollutants were detected by high performance liquid chromatography (HPLC). The main contents are as follows: chapter 1: high performance liquid chromatography (HPLC) technology and the characteristics, synthesis methods and applications of organometallic skeleton materials are reviewed. Chapter 2: the chlorophenoxy acid herbicide was extracted by dispersive liquid microextraction and labeled with 2-carbazole (a) carbazole-11-ethyl-4-methylbenzenesulfonic acid (BCETS) derivative reagent. It was determined by HPLC-FLD. The method has been successfully applied to the determination of 8 chlorophenoxy acid herbicides in crops by optimizing the extraction and derivation conditions. Chapter 3: UiO-67 organometallic skeleton materials were successfully synthesized and used as dispersive solid phase extraction adsorbents to extract and enrich several plant growth regulators. The principle of this kind of derivative reaction was explained by mass spectrometry, and the high sensitive detection of plant growth regulator in fruit was realized by optimizing the derivatization conditions and extraction conditions. Chapter 4: the JUC-48 metal-organic skeleton material with large pore size was successfully synthesized and its structure was identified by X-ray diffraction and scanning electron microscope. A sensitive detection method for polycyclic aromatic hydrocarbons (PAHs) was established by using the characteristics of large pore size and high specific area of the materials and the analysis of high performance liquid chromatography (HPLC). Seven polycyclic aromatic hydrocarbons (PAHs) in environmental water samples were successfully determined by this method. Chapter 5: the magnetic solid phase microextraction adsorbent Fe_3O_4@JUC-48 magnetic composite was successfully synthesized and used in the magnetic solid phase extraction of sulfanilamide antibiotics. The method is simple in operation, low in organic solvent consumption and high in extraction efficiency. Six sulfonamides antibiotics in meat food were detected by high performance liquid chromatography (HPLC).
【學(xué)位授予單位】：曲阜師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.72;TS207.53;X830

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本文編號：1947795