本文選題：海州香薷 + 叢枝菌根真菌(AMF)��； 參考：《山西師范大學》2017年碩士論文

【摘要】：土壤重金屬銅污染是全球環(huán)境污染治理的難題與焦點。AMF是一種能與大多數(shù)植物共生并且可以緩解重金屬脅迫對植物傷害的真菌。銅耐受植物海州香薷易與AMF形成良好的共生系統(tǒng)。本實驗以實驗室經(jīng)過二代重金屬銅脅迫的四個海州香薷種群:CK(無AMF,無Cu)、Cu(無AMF,有Cu)、AMF(有AMF,無Cu)、Cu+AMF(有AMF,有Cu)的種子為供試材料,通過框栽實驗分析多代重金屬銅脅迫及AMF對海州香薷適合度的影響,得到的結果如下:1.二代重金屬Cu脅迫的海州香薷Cu種群的種子表面積、周長、長度和寬度均顯著低于其他種群,而與二代重金屬Cu脅迫的海州香薷Cu種群的種子相比,接種AMF的Cu+AMF處理的種子則顯著增加了種子的表面積、周長、長度和寬度。二代重金屬Cu脅迫的海州香薷Cu種群的千粒重與其他種群相似。不管是在哪個銅濃度下,二代重金屬Cu脅迫的海州香薷Cu種群的種子的發(fā)芽指標和幼苗生長情況均顯著低于Cu+AMF處理二代的海州香薷種群種子。本實驗還發(fā)現(xiàn),Cu+AMF處理二代的海州香薷種群種子幼苗的胚根長度顯著高于其他種群,表明在受到二代重金屬Cu脅迫后,接種AMF可以幫助海州香薷幼苗通過提高胚根長度以增強幼苗對外界養(yǎng)分的捕獲能力,從而降低重金屬銅脅迫對后代適合度的抑制。2.受到了三代重金屬Cu脅迫的Cu處理與CK相比,顯著地抑制了植株的生長,降低了植株的株高、基徑、根生物量、莖生物量、葉生物量以及根系的總長度、根系的表面積、根系的平均直徑、根尖數(shù),而與Cu處理相比,添加AMF的Cu+AMF處理能顯著提高植株的各個形態(tài)指標,從而促進海州香薷的生長。3.與對照CK相比,經(jīng)過三代重金屬Cu脅迫的Cu處理延遲了植株的始花期、高峰期、終花期,縮短了花期持續(xù)時間,抑制了植物花序的長度和寬度,從而使得植株的種子總重量、種子總數(shù)量較少。與Cu處理相比,經(jīng)過三代重金屬Cu脅迫的Cu+AMF處理顯著提前了植株的各個花期,增加了花序長、花序?qū)�、花序�?shù)量、種子總重量、種子總數(shù)量。4.三代重金屬Cu脅迫的Cu處理植物與CK相比,顯著提高了植株根、莖、葉器官內(nèi)的重金屬Cu含量,提高了根中的P、K、Fe含量,莖中的P、Fe含量,而經(jīng)過三代重金屬Cu脅迫的Cu+AMF處理在添加了AMF后則顯著降低了根、莖、葉器官內(nèi)的重金屬Cu含量,降低了根中的P、S、K,莖中的P。
[Abstract]:Soil heavy metal copper pollution is a difficult problem and focus of global environmental pollution control. AMF is a fungus that can coexist with most plants and alleviate the damage of heavy metal stress to plants. The copper-tolerant plant Elsholtzia splendens is easy to form a good symbiotic system with AMF. In this experiment, the seeds of four populations of Elsholtzia splendens (No AMF, no Cu (AMF, Cu AMF) were used as the test materials in four populations of Elsholtzia splendens (Elsholtzia splendens) under heavy metal copper stress of the second generation. The effects of copper stress and AMF on the fitness of Elsholtzia splendens were studied in this paper. The results were as follows: 1. The seed surface area, circumference, length and width of the second generation of heavy metal Cu stress were significantly lower than those of the other populations, but compared with the second generation heavy metal Cu stress seeds of Elsholtzia splendens population. Cu AMF inoculated with AMF increased the surface area, perimeter, length and width of the seeds. The 1000-grain weight of Elsholtzia splendens population under Cu stress was similar to that of other populations. The germination index and seedling growth of the second generation of Elsholtzia splendens population under Cu stress were significantly lower than that of the second generation of Elsholtzia splendens population treated with Cu AMF. It was also found that the radicle length of the second generation of Elsholtzia splendens seed seedlings treated with Cu AMF was significantly longer than that of other populations, indicating that the second generation of heavy metal Cu stress was observed. Inoculation with AMF could help Elsholtzia splendens seedlings to increase the length of radicle to enhance their ability to capture external nutrients, and thus reduce the inhibition of copper stress on the suitability of progeny. 2. Compared with CK, Cu treatment under three generations of heavy metal Cu stress significantly inhibited plant growth, and decreased plant height, basal diameter, root biomass, stem biomass, leaf biomass, total root length and root surface area. Compared with Cu treatment, the average diameter of root system, the number of root tips, and Cu AMF added with AMF could significantly improve the morphological indexes of the plant, thus promoting the growth of Elsholtzia splendens. Compared with CK, Cu treatment of three generations of heavy metal Cu stress delayed the beginning of flowering, peak and final flowering, shortened the duration of flowering, inhibited the length and width of plant inflorescence, and resulted in the total weight of plant seeds. The total number of seeds is small. Compared with Cu treatment, Cu AMF treatment of three generations of heavy metal Cu stress significantly advanced the flowering period of the plant, and increased the length of inflorescence, the width of inflorescence, the number of inflorescence, the total weight of seeds and the total number of seeds. Compared with CK, Cu treatment of three generations of heavy metal Cu stress significantly increased the content of heavy metal Cu in plant root, stem and leaf organs, and increased the content of Pu K Fe in root and Pu Fe in stem. After three generations of Cu stress, Cu AMF significantly decreased the content of Cu in roots, stems and leaves, decreased the contents of AMF in roots and stems, and decreased the contents of Cu in stems.
【學位授予單位】：山西師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：X172;X173

【參考文獻】

相關期刊論文 前10條

1 楊麗麗;薛楠;李文匯;高欣;王柳;陳玉賀;;土壤銅對空心菜生長的影響研究[J];沈陽理工大學學報;2016年04期

2 李洋;于麗杰;金曉霞;;植物重金屬脅迫耐受機制[J];中國生物工程雜志;2015年09期

3 魚小軍;張建文;潘濤濤;張媛;丁爽;楊紅梅;肖紅;匡彥蓓;;銅、鎘、鉛對7種豆科牧草種子萌發(fā)和幼苗生長的影響[J];草地學報;2015年04期

4 王穗子;金則新;李月靈;谷銀芳;;銅脅迫條件下AMF對海州香薷光合色素含量、抗氧化能力和膜脂過氧化的影響[J];生態(tài)學報;2015年23期

5 陳俊任;柳丹;吳家森;李松;晏聞博;彭丹莉;葉正錢;王海龍;;重金屬脅迫對毛竹種子萌發(fā)及其富集效應的影響[J];生態(tài)學報;2014年22期

6 張麗坤;王朔;馮玉龍;;紫莖澤蘭種子形態(tài)特征和萌發(fā)特性與其入侵性的關系[J];生態(tài)學報;2014年13期

7 袁芳英;黃詠明;李莉;吳強盛;;菌根真菌對白三葉根系形態(tài)的影響及相關機理研究[J];長江大學學報(自科版);2014年11期

8 鄒英寧;吳強盛;李艷;黃詠明;;叢枝菌根真菌對枳根系形態(tài)和蔗糖、葡萄糖含量的影響[J];應用生態(tài)學報;2014年04期

9 張凡凡;于磊;魯為華;萬娟娟;;不同馴化年限野生線葉野豌豆種子特性及幼苗耐寒性[J];草業(yè)科學;2013年11期

10 李少朋;畢銀麗;陳f"圳;陳書琳;張延旭;孔維平;王瑾;;干旱脅迫下AM真菌對礦區(qū)土壤改良與玉米生長的影響[J];生態(tài)學報;2013年13期

相關碩士學位論文 前4條

1 李月靈;接種叢枝菌根真菌對海州香薷耐受銅脅迫的影響及機制[D];上海師范大學;2014年

2 茍靜宜;銅脅迫對紫鴨跖草生長發(fā)育的影響[D];四川師范大學;2010年

3 葉云山;銅對蔬菜的毒害效應及土壤銅臨界值研究[D];福建農(nóng)林大學;2009年

4 翟福勤;銅對作物幼苗的毒害機理及鐵鈣緩解銅毒害的研究[D];揚州大學;2007年

，

本文編號：1877324