本文選題：綠豆 + 硫酸銅�。� 參考：《植物生理學(xué)報(bào)》2016年12期

【摘要】：綠豆(Vigna radiata)在硫酸銅脅迫下水培發(fā)芽后再移植土種,觀察胚芽和幼苗生長情況;測定胚芽和幼苗細(xì)胞內(nèi)密度變化(DANCE反應(yīng)),以及胚芽的超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,研究萌發(fā)期硫酸銅脅迫對綠豆生長的影響及其DANCE反應(yīng)。結(jié)果顯示,硫酸銅脅迫抑制胚芽生長,以脅迫3~4 d最為顯著,并出現(xiàn)芽細(xì)胞DANCE反應(yīng)。移植土壤后,脅迫組幼苗出現(xiàn)代償性加快生長,生長率隨萌發(fā)期硫酸銅脅迫濃度增加而提高。土種3 d后,脅迫組幼苗表觀生長狀態(tài)恢復(fù)正常,但是,脅迫組幼苗根、莖、葉均出現(xiàn)DANCE反應(yīng)。根、莖、葉部在土種1~2 d后均發(fā)生DANCE反應(yīng),其中根部反應(yīng)持續(xù)1 d,莖部反應(yīng)持續(xù)3 d,而葉部反應(yīng)則持續(xù)至土種第5日。土種6 d后,各脅迫組幼苗均無DANCE反應(yīng)。硫酸銅脅迫還導(dǎo)致胚芽SOD活性和MDA含量顯著增高,SOD基因m RNA表達(dá)增加,提示硫酸銅脅迫促進(jìn)胚芽產(chǎn)生自由基。研究結(jié)果表明,綠豆的DANCE反應(yīng)可能是發(fā)芽減慢和幼苗代償性生長所致,發(fā)芽期適當(dāng)?shù)沫h(huán)境脅迫可能有助于植物幼苗生長。
[Abstract]:Vigna radiata was transplanted into soil after incubation and germination under copper sulfate stress, the growth of embryo and seedling was observed, the changes of intracellular density of germ and seedling were determined, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDAA) in germ were determined. The effects of copper sulfate stress during germination on the growth of mung bean and its DANCE response were studied. The results showed that cupric sulfate stress inhibited the growth of germ, and the DANCE reaction of bud cells appeared in the stress of 3 ~ 4 days. After transplanting soil, the seedlings of stress group developed modern compensatory growth, and the growth rate increased with the increase of copper sulfate stress concentration in germination period. After 3 days of soil planting, the apparent growth state of the seedlings in the stress group returned to normal, but DANCE reaction appeared in the roots, stems and leaves of the seedlings in the stress group. DANCE reaction occurred in root, stem and leaf after 1 ~ 2 days. The root reaction lasted 1 day, the stem reaction lasted 3 days, and the leaf reaction lasted until the 5th day. After 6 days, no DANCE response was found in the seedlings of each stress group. Copper sulfate stress also increased the activity of SOD and the content of MDA in germs, suggesting that copper sulfate stress could promote the production of free radicals. The results showed that the DANCE reaction of mung bean might be caused by slow germination and compensatory growth of seedlings, and proper environmental stress at germination stage might be helpful to plant seedling growth.
【作者單位】： 暨南大學(xué)醫(yī)學(xué)院生化系;廣州市東來生物科技有限公司;
【基金】：中國科技部科技創(chuàng)新基金(12C26214405312)~~
【分類號】：S522

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