本文選題：石莼 + 磺胺甲惡唑　； 參考：《中國(guó)環(huán)境科學(xué)》2017年08期

【摘要】：通過(guò)海水培養(yǎng)實(shí)驗(yàn),分析了磺胺甲惡唑(sulfamethoxazole,SMZ)和紅霉素(erythromycin,ETM)脅迫下石莼生長(zhǎng)、生理指標(biāo),探究石莼對(duì)SMZ和ETM的耐受性.并通過(guò)SMZ和ETM脅迫下石莼生理指標(biāo)主成分分析,篩選SMZ和ETM脅迫響應(yīng)表征指標(biāo).結(jié)果表明,低濃度SMZ(0.50mg/L)促進(jìn)石莼生長(zhǎng),相對(duì)生長(zhǎng)速率(RGR)顯著升高(P0.05),而ETM≥0.06mg/L抑制石莼生長(zhǎng).生理指標(biāo)間接反映石莼對(duì)SMZ和ETM的耐受性.較高濃度SMZ(≥2.50mg/L)和ETM(≥0.18mg/L)破壞石莼細(xì)胞膜透性,相對(duì)電導(dǎo)率顯著增加(P0.05);0.50~1.50mg/L SMZ和0.06~0.18mg/L ETM開始抑制石莼光合作用,葉綠素a(Chla)含量、1,6-二磷酸醛縮酶(FBA)活性顯著下降(P0.05);1.50mg/LSMZ和0.06mg/LETM破壞石莼抗氧化系統(tǒng)平衡,引起過(guò)氧化氫(H_2O_2)和丙二醛(MDA)含量顯著增加(P0.05),超氧化物歧化酶(SOD)、過(guò)氧化氫酶(CAT)、谷胱甘肽還原酶(GR)活性增加.綜合生長(zhǎng)和生理指標(biāo)表明,SMZ脅迫濃度不超過(guò)0.50mg/L對(duì)石莼生長(zhǎng)狀況和生理代謝有促進(jìn)作用,SMZ≥1.50mg/L,ETM≥0.06mg/L超過(guò)石莼耐受范圍,對(duì)石莼造成毒害,且石莼對(duì)SMZ的耐受性優(yōu)于ETM.主成分分析結(jié)果表明,表征SMZ脅迫下石莼細(xì)胞膜透性、光合作用、抗氧化系統(tǒng)變化的生理指標(biāo)分別為相對(duì)電導(dǎo)率,FBA和類胡蘿卜素(Car),MDA,而表征ETM脅迫下這3個(gè)生理過(guò)程的指標(biāo)分別為相對(duì)電導(dǎo)率,FBA和Car,MDA、CAT和抗壞血酸過(guò)氧化物酶(APX).
[Abstract]:The growth and physiological indexes of Ulva pertusa under stress of sulfamethoxazoleine (SMZ) and erythromycin (Erythromycin) were analyzed by seawater culture. The tolerance of Ulva pertusa to SMZ and ETM was studied. The principal component analysis of physiological indexes of Ulva pertusa under SMZ and ETM stress was used to screen SMZ and ETM stress response markers. The results showed that low concentration of SMZ 0.50 mg / L promoted the growth of Ulva pertusa, and the relative growth rate (RGR) increased significantly (P 0.05), while ETM 鈮，

本文編號(hào)：1821987