本文選題：緩流水體 + 脫氨氮菌��； 參考：《中原工學院》2017年碩士論文

【摘要】：針對黑臭緩流水體氨氮去除率低的問題,本研究采用改良富集培養(yǎng)基及現代菌株分離技術,分離培養(yǎng)高效脫氨氮菌株,生產制備菌劑,通過正交試驗,分析菌劑氨氮去除率的影響因素,考察菌劑運用于黑臭緩流水體的效果。在其他學者研究的基礎上,本研究對亞硝化菌富集用培養(yǎng)基進行了改進,在嚴格控制氨態(tài)氮含量的基礎上,加入紅糖,豐富碳素和微量元素。利用現代微生物分離技術,從氨氮去除率較高的污水處理廠和生活垃圾處理廠附近的污泥、污水中,分離出26株脫氮菌株,其中,16株以NH4+為氮源,分別記作X1-X16,10株以NO_2~-為氮源,分別記作菌株X17-X26。測定菌株生長速率,得到生長較快的菌株X1、X3、X4、X6、X17、X18、X19,經對菌株脫氮效率分析,篩選出氨氮去除效率最高的X1與X18混合菌株,該混合菌株的氨氮去除率可達90.0%。以紅糖為主要發(fā)酵原料,將該混合菌株擴大培養(yǎng),制備菌劑,并擬定菌劑的企業(yè)標準。模擬緩流水體的成分,在實驗室選取氨氮初始濃度、溫度、時間、pH、添加量、溶解氧6個因素,每個因素設置5個水平,通過正交試驗,分析脫氨氮菌劑使用過程中的影響因素,確定最佳菌劑最佳使用條件,極差分析結果顯示,影響菌劑氨氮去除率的六因素順序為溫度(29)溶解氧(29)氨氮初始濃度(29)添加量(29)時間(29)pH。確定本菌劑較佳使用條件為:溫度25℃,溶解氧濃度2mg/L,氨氮初始濃度40mg/L,菌劑添加量0.3%,時間120h,pH8,此條件下本菌劑氨氮去除率最高,可達94.7%。將菌劑應用于黑臭緩流水體,得出120h后水體中氨氮去除率為82.6%和85.0%,水樣嗅閾值明顯減小,除臭效果明顯;但與實驗室脫氮效果還有一定差距,菌劑對實驗室人工配水的氨氮去除率達94.7%,兩者相差近13.0%。其主要原因可能為實際緩流水體的成分較為復雜,水中存在抑制微生物菌劑生長的其他微生物群落或有害物質,對脫氨氮菌劑使用效果有一定影響。因此,可以對實驗室人工配水和緩流水體的性質進一步研究,不斷優(yōu)化菌劑的生長條件,進而提高菌劑用于緩流水體的氨氮去除率。
[Abstract]:Aiming at the problem of low removal rate of ammonia nitrogen in black-odor slow-flow water, this study adopted improved enrichment medium and modern strain isolation technology to isolate and culture high-efficiency denitrifying nitrogen strains, and to produce the preparation agent. The influence factors of ammonia-nitrogen removal rate were analyzed, and the effect of bacteria agent applied to black-odor slow-flow water was investigated. On the basis of other scholars' studies, the enrichment medium of nitrosobacteria was improved. On the basis of strictly controlling the content of ammonia nitrogen, brown sugar was added to enrich carbon and trace elements. Using modern microbial isolation technology, 26 denitrification strains were isolated from sewage treatment plants with high ammonia nitrogen removal rate and sludge near domestic waste treatment plants. Among them, 16 strains were denitrified with NH4 as nitrogen source, and 10 strains were recorded as X1-X16C- as nitrogen source. X17-X26. The growth rate of the strain X1, X3, X4, X4, X6, X17, X18, X19, was determined. The mixed strain X1 and X18, which had the highest removal efficiency of ammonia nitrogen, was screened by analyzing the denitrification efficiency of the strain. The ammonia nitrogen removal rate of the mixed strain could reach 90.0%. Using brown sugar as the main fermentation material, the mixed strain was expanded and cultured to prepare the fungicides, and the enterprise standard of the fungicides was worked out. The composition of the slow flow water was simulated, the initial concentration of ammonia nitrogen, temperature, time pH, addition amount, dissolved oxygen were selected in the laboratory, and each factor was set at 5 levels. Through orthogonal experiment, the influencing factors in the process of using ammonia nitrogen bacteria agent were analyzed. The results of range analysis showed that the six factors affecting the removal rate of ammonia-nitrogen were temperature (29) dissolved oxygen (29) initial concentration of ammonia (29) addition (29) time of 29? The optimum conditions were determined as follows: temperature 25 鈩，

本文編號：1821296