本文選題：石油降解菌 + 鎳抗性　； 參考：《生物技術(shù)通報》2017年10期

【摘要】：從四川省長寧-威遠地區(qū)頁巖氣開發(fā)井場重度污染區(qū)的土壤中,篩選出一株鎳抗性和石油高效降解菌株,研究其生物學(xué)特性和部分生理生化指標。將種子液接種在原油重金屬液體培養(yǎng)基中,30℃、130 r/min條件下分別培養(yǎng)7 d探究該菌株對金屬鎳離子的抗性和對總石油烴(Total petroleum hydrocarbon,TPH)的降解率,以及經(jīng)生理生化實驗、形態(tài)觀察和16S r DNA對該菌株鑒定分析。結(jié)果顯示,該菌株對Ni2+的耐受性可達300 mg/L,對Ni2+的去除率和吸附率分別達到了56.64%和52.16%,同時對總TPH的降解率達到35.65%。確定該菌種屬檸檬酸桿菌屬(Citrobacter sp.),命名為Citrobacter farmeri strain M1。該菌株的最適生長溫度為30℃,最適生長p H范圍為7-9,最適C∶N為10∶1和鹽最高耐受度可達5%。該菌株有較好的石油降解能力并對金屬鎳有較強的抗性,適用于石油重金屬混合污染土壤的修復(fù)。
[Abstract]:A strain of nickel resistance and high degradation of petroleum was screened from the soil of shale gas development well site in Changning-Weiyuan area of Sichuan Province. The biological characteristics and some physiological and biochemical indexes of the strain were studied.Seed solution was inoculated in crude oil heavy metal liquid medium at 30 鈩，

本文編號：1762637