本文選題：多環(huán)芳烴 + 中度嗜鹽菌��； 參考：《華東理工大學(xué)》2017年碩士論文

【摘要】：本研究針對(duì)采油區(qū)PAHs污染鹽堿土壤修復(fù)難度大、效率低的兩大突出問題,采用生物強(qiáng)化修復(fù)技術(shù)探索PAHs污染鹽堿土壤的修復(fù)方法,期望為我國(guó)油田地區(qū)PAHs污染鹽堿土壤的生物修復(fù)提供基礎(chǔ)數(shù)據(jù)。本研究分析了中度嗜鹽菌Martelellasp.AD-3降解芴、苊、苊烯、熒蒽等PAHs的降解特性,分析其主要代謝產(chǎn)物,闡明降解底物和代謝產(chǎn)物隨時(shí)間的變化過程,推測(cè)PAHs降解的代謝途徑;在分析比較土壤理化性質(zhì)的基礎(chǔ)上,利用高通量測(cè)序技術(shù)分析PAHs污染鹽堿土壤的微生物群落結(jié)構(gòu),鑒別PAHs污染鹽堿土壤中的優(yōu)勢(shì)菌群,并采用RT-PCR測(cè)定PAHs雙加氧酶基因含量;采用盆栽實(shí)驗(yàn)初步模擬研究AD-3與翅堿蓬聯(lián)合生物強(qiáng)化修復(fù)PAHs污染鹽堿土壤的修復(fù)效果。主要結(jié)果如下:(1)在3%鹽度、pH為9的條件下,中度嗜鹽菌Martelellasp.AD-3能分別以芴、苊、苊烯、熒蒽為唯一碳源和能源進(jìn)行降解,3天后芴(100mg/L)、苊(100mg/L)、苊烯(100mg/L)、熒蒽(20mg/L)的降解率分別為 78.0%、71.7%、85.7%、43.6%;補(bǔ)加酵母粉(50 mg/L)能夠促進(jìn)AD-3菌對(duì)芴和苊烯的降解,24 h內(nèi)可實(shí)現(xiàn)對(duì)100 mg/L芴和苊烯的完全降解。(2)利用HPLC、GC-MS、1HNMR等分析方法,分析AD-3菌降解芴、苊、苊烯的主要代謝產(chǎn)物。結(jié)果表明,芴的主要代謝產(chǎn)物有9-芴醇、9-芴酮、1,1a-二羥基-9-芴酮、鄰苯二甲酸;苊的主要代謝產(chǎn)物有1-苊醇、1-苊酮、1,2-二羥基苊、1-羥基-2-苊酮、1,8-萘二甲酸、1,8-萘二甲酸酐、1-萘酸;苊烯的主要代謝產(chǎn)物有1,2-二羥基苊、1-羥基-2-苊酮、苊醌、1,8-萘二甲酸、1-萘酸,并由此推測(cè)出相應(yīng)的代謝途徑。(3)翅堿蓬根際土壤鹽含量為22.51 g/kg,明顯低于裸地土壤的40.03 g/kg,總有機(jī)碳和N含量也均高于裸地土壤,分別到達(dá)24.41和1.59g/kg,證明翅堿蓬對(duì)根際土壤環(huán)境有改善作用;翅堿蓬根際土壤包含32門758屬細(xì)菌,多于裸地土樣28門676屬細(xì)菌,具有更高的微生物群落結(jié)構(gòu)多樣性,并且存在如Thioalkalispira、Halothiobacillus、Thiohalophilus等豐富的嗜鹽堿或耐鹽堿PAHs降解微生物資源。(4)翅堿蓬能在PAHs污染鹽堿土壤中發(fā)芽存活,發(fā)芽率為76.0-90.0%;中度嗜鹽菌Martelellasp.AD-3與翅堿蓬聯(lián)合作用的修復(fù)效果最佳,PAHs去除率達(dá)到92.0%,并且土壤中的微生物數(shù)量最高,數(shù)量級(jí)達(dá)10~8CFU/g,初步證明了該修復(fù)方法的可行性。
[Abstract]:Aiming at the two outstanding problems of PAHs polluted saline soil remediation in oil recovery area, which is difficult and low efficiency, the bioremediation technology was used to explore the remediation method of PAHs contaminated saline alkali soil.It is expected to provide basic data for the bioremediation of saline-alkali soils contaminated by PAHs in oil fields of China.In this study, the degradation characteristics of fluorene, acenaphthene, acenaphthene, fluoranthene and other PAHs degradation by moderately halophilic bacteria (Martelellasp.AD-3) were analyzed. The main metabolites were analyzed, and the process of degradation of substrate and metabolites with time was clarified, and the metabolic pathway of PAHs degradation was inferred.On the basis of analyzing and comparing the physical and chemical properties of soil, the microbial community structure of saline-alkali soil polluted by PAHs was analyzed by high-throughput sequencing technique, and the dominant flora in saline-alkali soil polluted by PAHs was identified, and the content of PAHs dioxygenase gene was determined by RT-PCR.Pot experiment was used to study the effect of AD-3 combined with Suaeda salsa on the remediation of saline-alkali soil contaminated by PAHs.The main results are as follows: (1) under the condition of 3% salinity and pH 9, the Martelellasp.AD-3 of moderately halophilic bacteria can be divided into fluorene, acenaphthene and acenaphthene, respectively.HPLCX GC-MS 1H NMR and other analytical methods were used.The main metabolites of fluorene, acenaphthene and acenaphthene degraded by AD-3 were analyzed.The main metabolites of acenaphthene are 1-hydroxy-2-acenaphthene ketone, acenaphthene quinone 1-8-naphthalene dicarboxylic acid,It was inferred that the soil salt content in the rhizosphere of Suaeda salsa was 22.51 g / kg, which was significantly lower than that in bare soil (40.03 g / kg), and the contents of total organic carbon and N were also higher than those in bare soil.Reaching 24.41 and 1.59 g / kg, respectively, it was proved that Suaeda salsa could improve the rhizosphere soil environment, and that the rhizosphere soil of Suaeda salsa contained 32 758 genera of bacteria, more than 28 phylum 676 genera of bacteria in bare soil, and had higher diversity of microbial community structure.And there are abundant salinophilic or saline-tolerant microbial resources, such as Thioalkalispiraus Halothiobacillus thiohalophilus, which can germinate and survive in saline-alkali soil polluted by PAHs.The germination rate was 76.0-90.0.The best removal rate of PAHs was 92.0 by the combination of moderate halophilic bacteria Martelellasp.AD-3 and Suaeda salsa, and the number of microbes in the soil was the highest, with an order of 10 ~ 8 CFU / g, which proved the feasibility of the remediation method.
【學(xué)位授予單位】：華東理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：X53

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本文編號(hào)：1742011