發(fā)布時(shí)間：2018-04-04 04:28

  本文選題：固定化　切入點(diǎn)：光合細(xì)菌　出處：《西南科技大學(xué)》2016年碩士論文

【摘要】：基于水體富營(yíng)養(yǎng)化嚴(yán)重,水體的生態(tài)功能降低,底泥作為凈化水體的二次污染源,水體底泥同步治理的重要性與固定化微生物技術(shù)的研究。本文的研究以特異性光合細(xì)菌為主體,采用幾種載體材料與方法將其固定,室內(nèi)模擬固定化光合細(xì)菌對(duì)水體與底泥中有機(jī)物、氮、磷的去除,研究了不同環(huán)境因素與人為因素對(duì)去除效果的影響。確定了主要的應(yīng)用條件及控制參數(shù),為固定化光合細(xì)菌技術(shù)在湖泊、水庫(kù)水體修復(fù)中的應(yīng)用提供理論和技術(shù)依據(jù)。主要實(shí)驗(yàn)結(jié)論如下:(1)論文研究對(duì)象光合細(xì)菌菌株多為短桿狀或卵圓形,寬0.5-0.9μm,長(zhǎng)1.2-2.0μm,無(wú)芽孢,無(wú)莢膜,單極鞭毛,可運(yùn)動(dòng)。培養(yǎng)一段時(shí)間后仍形態(tài)完整,表面光滑,分散較好,無(wú)團(tuán)聚。固定化載體材料多為多孔無(wú)毒礦物,具有較好的吸附性能和生物相容性。(2)光合細(xì)菌生長(zhǎng)特性研究中發(fā)現(xiàn),固定化光合細(xì)菌適宜生長(zhǎng)條件與游離光合細(xì)菌一致,均為30℃,p H 7-9,光照無(wú)氧。光合細(xì)菌經(jīng)固定化后生長(zhǎng)活性與生長(zhǎng)速率稍低于光合細(xì)菌懸液。光合細(xì)菌濃縮液與固定化顆粒水質(zhì)凈化最優(yōu)工藝條件:投加量20g·L-1,投加頻率4d·time-1,溫度30℃,完全光照,此時(shí)對(duì)水體中COD、NH3-N、TP的去除效果最高達(dá)到了80%左右。固定化后其適宜溫度范圍有所擴(kuò)大,25-30℃均具有較好的效果。(3)光合細(xì)菌經(jīng)無(wú)機(jī)載體固定化后細(xì)菌仍能保持原有形態(tài),作用一段時(shí)間后,上覆水中可檢測(cè)出大量光合細(xì)菌,未發(fā)生明顯變異。固定化光合細(xì)菌投入底泥后迅速擴(kuò)散,在底泥與上覆水之間的形成明顯的分層現(xiàn)象。分為上覆水層、底泥層與底泥和上覆水之間形成的一層較厚的微生物隔離層,實(shí)現(xiàn)光合細(xì)菌在水體的定殖。固定化顆粒在投加量為20g·L-1,投加頻率為4d·time-1時(shí),對(duì)底泥上覆水中的有機(jī)物、氨氮和總磷的去除效果最好。且固定化顆粒在去除底泥上覆水中有機(jī)物、氨氮和總磷的同時(shí)能消減底泥量。底泥消減量隨著投加量的增加而增大,在15g·L-1時(shí)對(duì)底泥量的消減效果最明顯。
[Abstract]:Based on the serious eutrophication of water body and the decrease of ecological function of water body, sediment as a secondary pollution source of purifying water body, the importance of simultaneous treatment of water sediment and the study of immobilized microorganism technology.In this paper, the specific photosynthetic bacteria were used as the main body and immobilized by several carrier materials and methods. The removal of organic matter, nitrogen and phosphorus in water and sediment was simulated by immobilized photosynthetic bacteria in laboratory.The effects of different environmental factors and human factors on the removal efficiency were studied.The main application conditions and control parameters were determined, which provided the theoretical and technical basis for the application of immobilized photosynthetic bacteria technology in the restoration of lakes and reservoirs.The main results were as follows: (1) the photosynthetic bacteria were mostly short rod or oval, 0.5-0.9 渭 m in width, 1.2-2.0 渭 m in length, no spore, no capsule, unipolar flagella, movable.After being cultured for a period of time, the shape is intact, the surface is smooth, the dispersion is better, and there is no agglomeration.The immobilized carrier materials are mostly porous nontoxic minerals, which have good adsorption performance and biocompatibility. It is found that the suitable growth conditions of immobilized photosynthetic bacteria are consistent with those of free photosynthetic bacteria.All of them were 30 鈩，

本文編號(hào)：1708368