XH02菌強(qiáng)化反應(yīng)器脫氮過程中菌群結(jié)構(gòu)的高通量分析
發(fā)布時間:2018-03-11 17:17
本文選題:異養(yǎng)硝化-好氧反硝化 切入點:生物多樣性 出處:《中國環(huán)境科學(xué)》2017年05期 論文類型:期刊論文
【摘要】:為了強(qiáng)化生物反應(yīng)器脫氮以及揭示微生物菌群結(jié)構(gòu)隨時間的動態(tài)變化,以O(shè)chrobactrum anthropi XH02和SBR反應(yīng)器為研究對象,利用16Sr DNA高通量測序技術(shù),對不同時期反應(yīng)器中微生物的菌群結(jié)構(gòu)和多樣性進(jìn)行了動態(tài)分析.研究結(jié)果表明,XH02菌的加入使得反應(yīng)器中TN和COD的去除率分別提升了15%和10%以上;反應(yīng)器中微生物菌群在屬水平上的相對豐度和多樣性呈先下降后上升的趨勢;XH02的加入對菌群結(jié)構(gòu)產(chǎn)生了較大影響.Acinetobacter、Blvii28和Aquabactenium菌的相對豐度顯著下降,而Fontibacter和Treponema菌的相對豐度則在強(qiáng)化過程中顯著升高;XH02的相對豐度隨著反應(yīng)器的運行逐漸增加,最后形成了較為穩(wěn)定的菌群;主成分分析和UPGMA聚類分析大致把反應(yīng)器運行過程分成4個階段.
[Abstract]:In order to enhance denitrification in bioreactor and reveal the dynamic change of microbial flora structure with time, Ochrobactrum anthropi XH02 and SBR reactors were used as research objects, and 16Sr DNA high-throughput sequencing technique was used. The microbial community structure and diversity in the reactor at different periods were dynamically analyzed. The results showed that the removal rates of TN and COD in the reactor were increased by more than 15% and 10% respectively with the addition of XH02 bacteria. The relative abundance and diversity of microbial flora in the reactor decreased first and then increased. The addition of XH02 had a great effect on the structure of the flora. The relative abundance of Acinetobacter Blvii28 and Aquabactenium decreased significantly. However, the relative abundance of Fontibacter and Treponema increased with the operation of the reactor, and a stable flora was formed. Principal component analysis (PCA) and UPGMA cluster analysis generally divide the reactor operation into four stages.
【作者單位】: 暨南大學(xué)環(huán)境學(xué)院;廣東省污染控制與修復(fù)材料工程技術(shù)中心;
【基金】:國家基金委-廣東省政府聯(lián)合基金重點項目(U1501233) 廣東省基金研究團(tuán)隊項目(2016A030312009) 廣東省環(huán)境污染控制與修復(fù)材料工程技術(shù)研究中心建設(shè)項目(2015B090903070)
【分類號】:X172;X703
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