本文選題：快速培養(yǎng)　切入點(diǎn)：高效反硝化細(xì)菌　出處：《環(huán)境科學(xué)》2017年09期 　論文類(lèi)型：期刊論文

【摘要】：培養(yǎng)高效的反硝化細(xì)菌可提高污水處理效率.本實(shí)驗(yàn)為序批式實(shí)驗(yàn),以Ⅰ號(hào)、Ⅱ號(hào)發(fā)酵液為碳源,采用梯度提高硝氮的方式,培養(yǎng)高效的反硝化細(xì)菌,從中選擇培養(yǎng)更加快速的發(fā)酵液.并采用高通量測(cè)序技術(shù)分析反硝化細(xì)菌的生物群落結(jié)構(gòu)和多樣性的變化.結(jié)果表明,Ⅱ號(hào)發(fā)酵液能夠在第11 d便實(shí)現(xiàn)高效反硝化細(xì)菌[300 mg·(L·h)-1]的快速培養(yǎng),比Ⅰ號(hào)發(fā)酵液提前了17 d,同時(shí),高效反硝化細(xì)菌系統(tǒng)對(duì)氨氮和總磷有一定的去除效果,最大去除速率分別為34.43 mg·(L·h)-1和2.98mg·(L·h)-1.高通量測(cè)序分析結(jié)果表明,污泥經(jīng)過(guò)馴化培養(yǎng),物種豐度和多樣性降低,但發(fā)揮反硝化作用的優(yōu)勢(shì)菌群的類(lèi)別和比例得到增大;細(xì)菌的組成及數(shù)量發(fā)生了較大的改變,最終發(fā)揮高效反硝化作用的核心菌屬為T(mén)hauera和Pseudomonas.另外,反硝化聚磷菌科(Rhodocyclaceae和Pseudomonadaceae)和異養(yǎng)硝化菌屬(Pseudomonas、Alcaligenes、Bacillus和Comamonas)的存在,驗(yàn)證了系統(tǒng)對(duì)氨氮和總磷的去除能力.
[Abstract]:The high efficiency denitrifying bacteria were cultured in the batch experiment, the fermentation broth I and II were used as carbon source, and the denitrifying bacteria were cultured by gradient increasing nitrate nitrogen. The fermentation broth was selected to grow faster. The changes of biome structure and diversity of denitrifying bacteria were analyzed by high-throughput sequencing technique. The culture of high efficiency denitrifying bacteria [300 mg 路L 路h ~ (-1)] was achieved on the 11th day, which was 17 days earlier than that of No. 1 fermentation broth. At the same time, the high efficiency denitrifying bacteria system could remove ammonia nitrogen and total phosphorus to a certain extent. The maximum removal rates were 34.43 mg 路L 路hn-1 and 2.98 mg 路L 路hn-1, respectively. The results of high-throughput sequencing showed that the species abundance and diversity decreased after the sludge was acclimated, but the type and proportion of dominant bacteria that played a role in denitrification were increased. The composition and quantity of bacteria changed greatly, and the core bacteria of high efficiency denitrification were Thauera and Pseudomonas. In addition, the presence of Rhodocyclaceae and Pseudomonadaceaeand Pseudomonas Alcaligenes Bacillus and Comonas. The removal capacity of ammonia nitrogen and total phosphorus was verified.
【作者單位】： 北京工業(yè)大學(xué)建筑工程學(xué)院北京市水質(zhì)科學(xué)與水環(huán)境恢復(fù)工程重點(diǎn)實(shí)驗(yàn)室;
【分類(lèi)號(hào)】：X172;X703

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本文編號(hào)：1596230