本文關(guān)鍵詞：熒光探針在小分子環(huán)境污染物檢測中的應(yīng)用研究　出處：《青島科技大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 熒光探針 羅丹明6G 四氧化三鐵 鉛離子 鐵離子

【摘要】：熒光檢測法成為近年來的研究熱點,利用熒光探針檢測環(huán)境水體、大氣及土壤中的重金屬離子的含量已得到廣泛應(yīng)用,該方法的優(yōu)點主要體現(xiàn)在檢測快速,操作簡單、信號明顯。本論文設(shè)計合成出兩種類型熒光探針,可分別用于鉛離子和三價鐵離子的檢測,具體內(nèi)容如下:(1)以羅丹明6G熒光染料為母體,設(shè)計合成出新型有機(jī)熒光探針R1,并采用紅外光譜、核磁共振氫譜、核磁共振碳譜、高分辨質(zhì)譜及元素分析法對合成出的熒光探針進(jìn)行了表征。該合成路線簡單,產(chǎn)率高,實驗條件溫和,易于分離,且儲存條件要求不高,溫度保持在0-4℃即可。(2)熒光探針R1對水相中的鉛離子具有很高的選擇性和靈敏度,屬于熒光增強(qiáng)型探針,鉛離子的加入可使探針溶液顏色由亮黃色變?yōu)榉凵?實現(xiàn)了對鉛離子的可視化檢測。其識別原理為Pb~(2+)誘導(dǎo)探針分子結(jié)構(gòu)中內(nèi)酰胺螺環(huán)打開,熒光由無到有,探針R1與Pb~(2+)1:1形式絡(luò)合,在鉛離子濃度范圍為1.0×10-8-1.0×10-5mol L-1時熒光強(qiáng)度與鉛離子濃度成線性相關(guān),檢出限為2.7×10-9mol L-1(S/N=3)。(3)利用化學(xué)共沉淀法制備出四氧化三鐵納米顆粒,制備的四氧化三鐵顆粒為球形,大小約為50 nm,通過表面包敷和巰基乙酸處理實現(xiàn)對Fe_3O_4顆粒表面的功能化修飾,形成巰基乙酸修飾的四氧化三鐵復(fù)合納米顆粒,該復(fù)合顆�？勺鳛闄z測Fe3+的熒光探針,屬于熒光淬滅型探針,顆粒表面的巰基絡(luò)合Fe3+,致使其熒光強(qiáng)度降低,熒光強(qiáng)度在Fe3+濃度為5.0×10-6~7.0×10-5mol L-1范圍內(nèi)線性相關(guān),其檢出限為1.8×10-7 mol L-1。該巰基化四氧化三鐵復(fù)合納米顆粒合成方法簡單,實驗易操作且成本較低,作為三價鐵離子熒光探針,具有良好的選擇性和較高的靈敏度。
[Abstract]:Fluorescence detection method has become a hot research topic in recent years. Using fluorescent probe to detect heavy metal ions in environmental water, atmosphere and soil has been widely used. The advantages of this method are mainly reflected in rapid detection. In this paper, two types of fluorescent probes were designed and synthesized, which can be used for the detection of lead ion and trivalent iron ion respectively. The specific contents are as follows: 1) Rhodamine 6G fluorescent dye is used as the parent. A novel organic fluorescence probe R1 was designed and synthesized. The infrared spectra, nuclear magnetic resonance (NMR) spectra and nuclear magnetic resonance (NMR) carbon spectra were used. The synthesized fluorescent probe was characterized by high resolution mass spectrometry and elemental analysis. The synthetic route is simple, the yield is high, the experimental conditions are mild, the separation conditions are easy, and the storage conditions are not high. The fluorescence probe R1 has high selectivity and sensitivity to lead ions in aqueous phase, and is a fluorescence enhanced probe. The color of probe solution can be changed from bright yellow to pink with the addition of lead ion, and the visual detection of lead ion can be realized. The recognition principle is Pb~(2) to induce the opening of lactam snail ring in the molecular structure of probe. The fluorescence of the probe R1 is in the form of 1: 1 Pb~(2. The fluorescence intensity is linearly correlated with lead ion concentration when lead ion concentration ranges from 1.0 脳 10 ~ (-8) to 1.0 脳 10 ~ (-5) mol 路L ~ (-1). The detection limit is 2.7 脳 10 ~ (-9) mol / L ~ (-1) S / N _ (3) O _ (3). Fe _ 2O _ 3 nanoparticles were prepared by chemical coprecipitation, and the Fe _ 2O _ 3 nanoparticles were spherical. The surface of Fe_3O_4 particles was functionalized by surface encapsulation and thioglycolic acid treatment, and the modified Fe _ 2O _ 3 nanoparticles were formed by thioglycolic acid. The composite particle can be used as a fluorescence probe for detecting Fe3, which belongs to the fluorescence quenching type. The mercapto complex Fe3 on the surface of the particle decreases its fluorescence intensity. The fluorescence intensity was linearly correlated with the concentration of Fe3 in the range of 5.0 脳 10 ~ (-6) mol 路L ~ (-1) to 7.0 脳 10 ~ (-5) mol / L. The detection limit is 1.8 脳 10 ~ (-7) mol / L ~ (-1). The method is simple, easy to operate and low cost. It can be used as a fluorescence probe for trivalent iron ions. It has good selectivity and high sensitivity.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：X830.2;O657.3

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