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  本文關鍵詞：右旋酪氨酸及糖苷水解酶緩解膜系統(tǒng)中膜污染的研究　出處：《山東大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 膜生物污染 右旋氨基酸 PslG 抗污染性能

【摘要】：膜污染是膜分離技術中存在的一個瓶頸問題,限制了膜技術的進一步發(fā)展。因此,開發(fā)緩解膜污染的新技術方法對膜技術至關重要。本研究選用生物自身分泌的兩種物質(zhì)右旋酪氨酸和糖苷水解酶PslG,探究它們對膜污染的抑制作用并解析作用機理。采用一種簡單溫和的兩步法將右旋酪氨酸修飾到聚醚砜(PES)膜表面,通過增加膜表面親水性及抗菌性,進而緩解膜生物污染。結(jié)果顯示,右旋酪氨酸修飾后能夠增加膜表面親水性、降低粗糙度,并且不會對膜的滲透性能產(chǎn)生影響。在牛血清白蛋白(BSA)的過濾實驗中,D-tyrosine-PDA/PES膜總的統(tǒng)一膜污染指數(shù)(UMFI)是0.00162,僅為PDA/PES膜UMFI的一半。同時,D-tyrosine-PDA/PES膜的水通量恢復率(FRR)增加至79%,高于原始PES膜的FRR。修飾后,膜表面細菌粘附率由29.2%降到19%,抗粘附性能增加。細菌污染前后,膜的水通量對比圖也說明修飾后膜表面抗生物污染性能增加。這種膜表面改性的方法可以同時增強膜的抗粘附及抗菌性能,實現(xiàn)了用生物方法緩解膜污染。本章研究又采用直接投加法,探究糖苷水解酶PslG對膜污染的控制作用。細菌粘附實驗結(jié)果表明,在較短時間內(nèi),PslG能明顯抑制細菌在膜表面粘附,且抑制效果與加入的PslG濃度沒有關系。PslG的存在并不會影響細菌表面zeta電位及疏水率。24h的粘附實驗顯示,投加高濃度的PslG(500、1000nM)才會對細菌粘附產(chǎn)生明顯的抑制作用。同時cross-flow過濾實驗說明,PslG能夠緩解過濾過程中產(chǎn)生的膜生物污染。PslG的存在減少了菌液EPS中胞外多糖的分泌,這也是其抑制細菌粘附、緩解膜生物污染的原因。
[Abstract]:Membrane fouling is a bottleneck problem in membrane separation technology, which restricts the further development of membrane technology. Therefore, the development of new technology to alleviate membrane pollution is very important to membrane technology. In this study, two kinds of dextral tyrosine and glucoside hydrolase PslG, which were secreted by biological self - secreting, were used to explore their inhibitory effects on membrane pollution and to analyze the mechanism. A simple mild two step method was applied to modify the dextran tyrosine onto the surface of polyethersulfone (PES) membrane, which increased membrane surface hydrophilicity and antibacterial activity, and further alleviated membrane biofouling. The results show that the modification of Dextre tyrosine can increase the hydrophilicity of the membrane surface, reduce the roughness, and do not affect the permeability of the membrane. In the filtration experiment of bovine serum albumin (BSA), the total uniform membrane fouling index (UMFI) of the D-tyrosine-PDA/PES membrane was 0.00162, only half of the UMFI of the PDA/PES membrane. At the same time, the recovery rate of water flux (FRR) of the D-tyrosine-PDA/PES film increased to 79%, which was higher than that of the original PES film FRR. After modification, the bacterial adhesion rate of the membrane surface decreased from 29.2% to 19%, and the adhesion resistance increased. Before and after the bacterial contamination, the water flux contrast diagram of the membrane also showed that the surface resistance of the modified membrane was increased. The membrane surface modification method can enhance the anti adhesion and antibacterial properties of the membrane at the same time, and the membrane fouling can be alleviated by biological method. In this chapter, the direct addition method is used to explore the control effect of glycoside hydrolase PslG on membrane pollution. The results of bacterial adhesion test showed that PslG could obviously inhibit the adhesion of bacteria on the membrane surface in a short time, and the inhibition effect was not related to the concentration of PslG. The presence of PslG does not affect the surface zeta potential and hydrophobicity of the bacteria. The adhesion test of 24h showed that high concentration of PslG (500, 1000nM) could inhibit the adhesion of bacteria. At the same time, the cross-flow filtration experiment showed that PslG could alleviate the biofilm pollution produced during the filtration process. The presence of PslG reduces the secretion of extracellular polysaccharide in the bacterial liquid EPS, which is also the reason for its inhibition of bacterial adhesion and biofilm contamination.
【學位授予單位】：山東大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：X505

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