本文關(guān)鍵詞：上丘-丘腦外側(cè)后核神經(jīng)環(huán)路在處理本能恐懼反應(yīng)中的作用研究　出處：《中國科學(xué)院深圳先進(jìn)技術(shù)研究院》2016年碩士論文　論文類型：學(xué)位論文

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【摘要】：動(dòng)物能對(duì)逐漸靠近自己的視覺危險(xiǎn)刺激做出本能快速的防御性反應(yīng),行為學(xué)上主要表現(xiàn)為凍結(jié)和逃跑。依據(jù)本實(shí)驗(yàn)室前期的工作發(fā)現(xiàn),小鼠對(duì)這種特定視覺刺激產(chǎn)生的本能恐懼行為,是通過上丘(superior colliculus,SC)、丘腦外側(cè)后核(lateral posterior nucleus of thalamus,LP)到杏仁核這條快速的皮層下通路來介導(dǎo)。基于這些研究基礎(chǔ),我們希望理解的問題是:1)特定的、可以誘發(fā)本能恐懼行為的視覺輸入信息在SC中編碼后,這些視覺輸入中的危險(xiǎn)信息成分如何在SC到LP的通路中被抽提和加工;2)LP接受SC信息輸入的功能亞區(qū)和神經(jīng)元發(fā)放的特征。本研究以小鼠為實(shí)驗(yàn)對(duì)象,利用多種技術(shù)手段,包括本能恐懼行為檢測、免疫組化分析、光遺傳神經(jīng)調(diào)控技術(shù)、在體多通道電生理記錄以及在體光纖熒光信號(hào)檢測等技術(shù),對(duì)上述問題進(jìn)行了研究。初步結(jié)果發(fā)現(xiàn):1)用不同頻率光刺激模擬不同強(qiáng)度的視覺刺激輸入到上丘中層內(nèi)側(cè)(medial region of the intermediate layers of the SC,ILSCm)興奮性神經(jīng)元,能誘導(dǎo)小鼠產(chǎn)生防御性逃跑或凍結(jié)反應(yīng)。而隨著刺激頻率的增加,小鼠的行為逐漸從凍結(jié)趨向于逃跑行為。因此,ILSCm興奮性神經(jīng)元活動(dòng)的強(qiáng)弱在小鼠不同本能防御行為編碼中起著很重要的作用。2)光遺傳學(xué)激活SC到LP不同亞區(qū)的投射通路,發(fā)現(xiàn)刺激LPMR(mediorostral of lateral posterior thalamic nucleus)和LPMC/LPLR(mediocaudal or laterorostral of lateral posterior thalamic nucleus)亞區(qū)都能誘發(fā)小鼠產(chǎn)生凍結(jié)行為,但是LPMC/LPLR誘發(fā)凍結(jié)的時(shí)間更長,因此這提示LPMC/LPLR可能是接受上丘威脅刺激信號(hào)的主要功能亞區(qū)。為了驗(yàn)證這一假設(shè),我們利用在體多通道電生理和光纖熒光檢測技術(shù),記錄了looming刺激下LP不同亞區(qū)神經(jīng)元的活動(dòng),結(jié)果發(fā)現(xiàn)LPLR對(duì)looming刺激沒有反應(yīng)而LPMR和LPMC則有,并且LPMC神經(jīng)元活動(dòng)與looming刺激誘導(dǎo)的防御行為具有相關(guān)性。因此,LPMC可能是接受上丘威脅信號(hào)的主要功能亞區(qū)。本文研究了SC和LP神經(jīng)通路如何編碼looming刺激引起的本能防御反應(yīng),進(jìn)一步揭示了這條非經(jīng)典的皮層下通路在對(duì)本能恐懼情感處理中的作用機(jī)制,這將為臨床上開發(fā)治療與恐懼異常類相關(guān)精神疾病提供新的思路和參考。
[Abstract]:Animals are able to react instinctively and defensively to visual hazard stimuli gradually approaching themselves, primarily in the form of freezes and escapes, according to previous work in our laboratory. The instinctive fear of this particular visual stimulus in mice is through the superior colliculus colliculus (SCS). Lateral posterior nucleus of thalamus. LP) is mediated by the rapid subcortical pathway to the amygdala. Based on these studies, the question we want to understand is: 1) specific. After the visual input information which can induce instinctive fear behavior is encoded in SC, how can the dangerous information components of these visual inputs be extracted and processed in the pathway from SC to LP; 2the functional subregion of SC information input and the characteristics of neuronal release were accepted by LP. In this study, mice were used as experimental subjects, using a variety of technical means, including instinctive fear behavior detection, immunohistochemical analysis. Photogenetic neural regulation technology, in vivo multi-channel electrophysiological recording and in vivo optical fiber fluorescence signal detection technology. The preliminary results show that different frequency of light stimulation is used to simulate visual stimulation of different intensity into the medial medial medial colliculus. Medial region of the intermediate layers of the SC. ILSCm) excitatory neurons can induce defensive escape or freeze response in mice. With the increase of stimulation frequency, the behavior of mice gradually changes from freezing to escaping. The activity of ILSCm excitatory neurons plays an important role in the encoding of different instinctive defense behaviors in mice. 2) photogenetics activates the projection pathway from SC to different subregions of LP. LPMR(mediorostral of lateral posterior thalamic nucleus. And LPMC / LPLR (. Mediocaudal or laterorostral of lateral posterior thalamic nucleus. All the subregions could induce freezing behavior in mice. But LPMC/LPLR induces freezing for longer, which suggests that LPMC/LPLR may be the main functional subregion that receives the superior colliculus threat stimuli. We recorded the activity of neurons in different subregions of LP stimulated by looming using in vivo multichannel electrophysiology and fiber-optic fluorescence detection techniques. The results showed that LPLR did not respond to looming stimulation, but LPMR and LPMC did. And the activity of LPMC neurons is related to the defense behavior induced by looming stimulation. LPMC may be the main functional subregion to receive the superior colliculus threat signal. We studied how SC and LP neural pathways encode the instinctive defense response induced by looming stimulation. It further reveals the mechanism of this nonclassical subcortical pathway in the treatment of instinctive fear emotion, which will provide new ideas and references for clinical treatment of mental disorders associated with fear abnormality.
【學(xué)位授予單位】：中國科學(xué)院深圳先進(jìn)技術(shù)研究院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：B842

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1 容康林;上丘-丘腦外側(cè)后核神經(jīng)環(huán)路在處理本能恐懼反應(yīng)中的作用研究[D];中國科學(xué)院深圳先進(jìn)技術(shù)研究院;2016年

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