【摘要】：目的優(yōu)化姜黃素(Cur)納米粒凍干粉(CNLP)制備工藝。方法以丙酮作為溶劑,去離子水作為反溶劑,聚山梨酯-80(P80)作為表面活性劑,甘露醇作為凍干保護劑通過反溶劑法制備CNLP,采用單因素分析法進行CNLP制備工藝的優(yōu)化。影響CNLP粒徑大小的因素有Cur質量濃度、溶劑與反溶劑體積比、表面活性劑用量、沉積時間、攪拌速率、凍干保護劑用量;得到的CNLP與原粉進行飽和溶解度和體外溶出對比實驗。結果制備CNLP最優(yōu)工藝為Cur質量濃度8mg/m L、溶劑與反溶劑比1∶5、表面活性劑用量0.05%、沉積時間5 min、攪拌速率400 r/min、凍干保護劑甘露醇與Cur質量比4∶1;獲得的CNLP平均粒徑大小為(172.2±4.6)nm;CNLP復溶液Zeta電位為(-19.7±3.7)m V;SEM圖譜顯示Cur原粉呈不規(guī)則的大塊狀,粒徑在20μm左右。CNLP呈不規(guī)則的均勻塊狀結構,粒徑在170 nm左右,與原粉相比粒徑明顯減小。從以上CNLP的形態(tài)來看,由激光粒度儀測得的CNLP平均粒徑是基本一致的。通過飽和溶解度檢測,在去離子水中,CNLP的飽和溶解度是原粉的41.32倍;在人工胃液中,CNLP飽和溶解度是原粉的1.74倍;在人工腸液中,CNLP飽和溶解度是原粉的4.11倍。通過溶出度檢測,在去離子水中,CNLP的溶出速率是原粉的14.51倍;在人工胃液中,CNLP的溶出速率是原粉的2.33倍;在人工腸液中,CNLP的溶出速率是原粉的44.79倍。結論反溶劑法制備的CNLP可以改善Cur水溶性,有利于提高Cur的生物利用度。
[Abstract]:Objective to optimize the preparation of curcumin (Cur) nanoparticles freeze-dried powder (CNLP). Methods CNLP, was prepared with acetone as solvent, deionized water as anti-solvent, polysorbate-80 (P80) as surfactant and mannitol as freeze-drying protectant to prepare CNLP by anti-solvent method. The factors influencing the particle size of CNLP include the mass concentration of Cur, the volume ratio of solvent to antisolvent, the dosage of surfactant, the deposition time, the stirring rate and the dosage of freeze-dried protectant, and the saturated solubility and dissolution in vitro of the obtained CNLP are compared with that of the original powder. Results the optimum conditions for preparing CNLP were as follows: the mass concentration of Cur was 8mg/m / L, the ratio of solvent to antisolvent was 1? 5, the dosage of surfactant was 0.05%, the deposition time was 5 min, the stirring rate was 400r / min, and the mass ratio of mannitol to Cur was 4? The average particle size of CNLP was (172.2 鹵4.6) nm;CNLP. The Zeta potential of complex solution was (- 19.7 鹵3.7) m V;). The SEM pattern showed that the Cur powder was irregular and large, the particle size was about 20 渭 m, and the size of the raw powder was about 170 nm, which was smaller than that of the original powder. The size of the raw powder was about 20 渭 m, and the size of the powder was about 170 nm. According to the morphology of CNLP, the average particle size of CNLP measured by laser particle sizer is basically the same. The saturated solubility of CNLP in deionized water was 41.32 times that of original powder, the saturated solubility of CNLP in artificial gastric juice was 1.74 times higher than that of original powder, and the saturated solubility of CNLP in artificial intestinal fluid was 4.11 times of that of original powder. The dissolution rate of CNLP in deionized water was 14.51 times that of original powder, the dissolution rate of CNLP in artificial gastric juice was 2.33 times of that of original powder, and the dissolution rate of CNLP in artificial intestinal fluid was 44.79 times of that of original powder. Conclusion CNLP prepared by anti-solvent method can improve the water solubility of Cur and increase the bioavailability of Cur.
【作者單位】： 東北林業(yè)大學森林植物生態(tài)學教育部重點實驗室;
【基金】：中央高�；究蒲袠I(yè)務費專項資金項目(2572014AB11) 國家自然科學基金資助項目(21473023)
【分類號】：R283.6
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本文編號：2460252