【摘要】：為篩選適用于發(fā)酵蛤蜊制備降血壓活性多肽的納豆芽孢桿菌,本試驗從7種日本納豆中初篩6株具有較高蛋白酶活性的納豆芽孢桿菌株用于發(fā)酵蛤蜊,以體外血管緊張素轉(zhuǎn)化酶(ACE)抑制率及多肽含量為評價指標(biāo)篩選目的菌株。結(jié)果表明,共篩選出3株目的菌株(GL-5、GL~(-1)2和GL-25),其發(fā)酵產(chǎn)物對ACE抑制率分別為71.55%、78.31%、75.08%,多肽含量分別可達(dá)8.12、9.62、8.79mg·m L~(-1)。其中,GL~(-1)2發(fā)酵產(chǎn)物經(jīng)消化酶水解后仍保持68.31%的ACE抑制率,表明其具有較強的抗消化能力。本研究結(jié)果為蛤蜊高值化利用開發(fā)具有降血壓作用的功能食品提供了一定的理論依據(jù)。
[Abstract]:In order to screen Bacillus natto, which is suitable for fermentation of antihypertensive peptides from clams, 6 strains of Bacillus natto with high protease activity were screened out from seven natto species to ferment clams, and 6 strains of Bacillus natto with high protease activity were screened from 7 natto species. The inhibition rate of angiotensin converting enzyme (ACE) and the content of polypeptide in vitro were used as the evaluation index to screen the target strains. The results showed that three target strains (GL-5,GL~ _ (- 1) _ 2 and GL-25) were screened, and their inhibition rates on ACE were 71.55%, 78.31% and 75.08%, respectively. The contents of peptides were 8.12,9.62 and 8.79mg 路mL ~ (- 1), respectively. Among them, GL~ (- 1) 2 fermentation product still kept 68.31% inhibition rate of ACE after hydrolyzed by digestive enzyme, which indicated that it had strong anti-digestion ability. The results of this study provide a theoretical basis for the high value utilization of clam to develop a functional food with hypotensive effect.
【作者單位】： 青島大學(xué)生命科學(xué)學(xué)院;榮成泰祥食品股份有限公司;
【基金】：山東省重點研發(fā)計劃(重大關(guān)鍵技術(shù))(2016ZDJS06A01)
【分類號】：TQ464.7;TQ920.1

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