本文選題：乙醇沉淀法 + 人血白蛋白��； 參考：《長春中醫(yī)藥大學(xué)》2011年碩士論文

【摘要】：目的:考察不同的制備人血白蛋白微球的方法,選擇適于工業(yè)化生產(chǎn)的制備方法,研究該方法制備不同粒徑人血白蛋白微粒的最佳條件,并對于其裝載人參皂苷Rb1、阿霉素、異硫氰酸熒光素、銀耳多糖4種性質(zhì)不同藥物時方法的適用性、制備的微球性質(zhì)進(jìn)行考察。 方法:采用乙醇沉淀法制備不同粒徑的微粒,采用吸附法和結(jié)合法裝載不同種類的藥物。使用HPLC法測定人參皂苷Rb1、阿霉素、異硫氰酸熒光素的載藥量,采用分光光度計法測定銀耳多糖的載藥量。并通過透析法、酶解法、凍干法考察人參皂苷Rb1人血白蛋白微球的釋藥性能、體外降解性能和儲存工藝。 結(jié)果:采用乙醇沉淀法制備出3種粒徑區(qū)間的微粒,分別為:“100nm以下”、“400nm-1000nm”、“1μm-2μm”。并可以裝載人參皂苷Rb1、阿霉素、異硫氰酸熒光素、銀耳多糖。載人參皂苷Rb1和銀耳多糖的人血白蛋白微粒載藥量較高,載阿霉素和異硫氰酸熒光素的人血白蛋白微粒載藥量較低。人參皂苷Rb1人血白蛋白微球具有明顯的緩釋性能,可調(diào)節(jié)其體外降解時間,凍干后在4℃下穩(wěn)定的保存6個月。 結(jié)論:適宜采用乙醇沉淀法制備成載人參皂苷Rb1和銀耳多糖的人血白蛋白微粒,這些藥物水溶性好、化學(xué)性質(zhì)穩(wěn)定、藥物性質(zhì)為中性,并可按照需要制備不同粒徑、不同載藥量、不同降解時間的微粒。不適宜制備載阿霉素等具有較活潑化學(xué)集團(tuán)、可使蛋白變性的藥物。對異硫氰酸熒光素等水溶性較差藥物,本方法也有一定的局限性。
[Abstract]:Objective: to investigate the different preparation methods of human serum albumin microspheres, to select the suitable preparation method for industrial production, to study the optimum conditions for the preparation of different particle sizes of human serum albumin microspheres, and to study the loading of ginsenoside Rb1, doxorubicin for the preparation of human serum albumin microspheres. The applicability of four different methods for the preparation of fluorescein isothiocyanate and Tremella polysaccharide were investigated. Methods: different particle sizes were prepared by ethanol precipitation, and different kinds of drugs were loaded by adsorption and binding methods. The drug loading of ginsenoside Rb1, doxorubicin and fluorescein isothiocyanate was determined by HPLC, and the drug loading of Tremella polysaccharide was determined by spectrophotometer. The drug release, in vitro degradation and storage of ginsenoside Rb1 human albumin microspheres were investigated by dialysis, enzymatic hydrolysis and lyophilization. Results: three kinds of particle sizes were prepared by ethanol precipitation, which were "below 100nm", "400nm-1000nm" and "1 渭 m -2 渭 m". Ginsenoside RB 1, adriamycin, fluorescein isothiocyanate, Tremella polysaccharide can be loaded. The amount of human serum albumin particles loaded with ginsenoside Rb1 and Tremella polysaccharide was higher than that with doxorubicin and fluorescein isothiocyanate. Ginsenoside Rb1 human serum albumin microspheres have obvious slow-release properties, and can adjust the degradation time in vitro. After freeze-drying, the microspheres are kept steadily at 4 鈩，

本文編號：1915348