本文選題：西漢古酒 + 菌害分析　； 參考：《西北大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年06期

【摘要】：針對(duì)2003年西安市文物保護(hù)考古所發(fā)現(xiàn)的西安棗園西漢早期墓葬出土古酒的菌害,分析微生物群落,并對(duì)照其成份判斷菌種來源,為制定穩(wěn)妥的古酒滅菌方案提供科學(xué)依據(jù)。研究使用分子生物學(xué)方法,通過培養(yǎng)分離微生物群落,16S r DNA/18S r DNA序列分析,根據(jù)同源性比對(duì)及系統(tǒng)發(fā)育樹分析,鑒定出4種優(yōu)勢(shì)菌種,其中真菌為淡紫擬青霉菌,3種細(xì)菌分別為微球菌、葡萄球菌和考克氏菌屬細(xì)菌。鑒定出的4種優(yōu)勢(shì)菌種與空氣微生物中的常見菌種一致;判定古酒菌害原因主要來源于外界空氣的交流;提出通過菌落濾除,隔絕空氣,以有效抑制古酒中微生物滋生的防治對(duì)策。該研究也是16S r DNA/18S r DNA序列分析首次成功在酒類液體文物菌害成份鑒定中應(yīng)用。
[Abstract]:In view of the bacteria damage of ancient wine unearthed from the early Western Han Dynasty tomb in Xi'an Zaoyuan in 2003, the microbial community was analyzed, and the source of bacteria was judged in comparison with its composition, which provided a scientific basis for the formulation of a sound sterilization plan for ancient wine. By using molecular biology method, four dominant strains were identified by sequence analysis of 16s r DNA/18S r DNA in culture and isolation microbial community, according to homology comparison and phylogenetic tree analysis. Among them, the fungus is Penicillium lilacinus and three kinds of bacteria are micrococcus, Staphylococcus and Corkella respectively. The four dominant strains identified are consistent with the common strains of airborne microbes; the main reasons for the bacteria damage in ancient wine are derived from the exchange of external air; and the idea of isolating the air through colony filtration is put forward. In order to effectively inhibit the microbial breeding in ancient wine control measures. This study is also the first successful application of 16s r DNA/18S r DNA sequence analysis in the identification of the harmful components of alcoholic liquid cultural relics.
【作者單位】： 西安市文物保護(hù)考古研究院;西北大學(xué)生命科學(xué)學(xué)院;
【基金】：陜西省生物技術(shù)重點(diǎn)實(shí)驗(yàn)室基金資助項(xiàng)目(J16S108)
【分類號(hào)】：K875.2;TS261.7
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本文編號(hào)：1927445