【摘要】： 目的通過對DYS713、DYS723 Y-STR基因座在山西漢族群體的研究,獲得各基因座的基因頻率分布情況,為群體遺傳學、法醫(yī)學個體識別及親子鑒定提供計算數(shù)據(jù),對其在法醫(yī)學上的應用進行可行性探討。 方法從GDB數(shù)據(jù)庫中尋找DYS713、DYS723 Y-STR基因座;用PCR方法擴增,電泳分型;對其所有等位基因測序,構建等位基因分型標準物;按照ISFG原則命名各等位基因。 結果(1)在本研究的群體樣本中,DYS713、DYS723基因座分別觀察到6、5個等位基因,其基因變異度分別為0.7370、0.6618,其個人識別能力和非父排除率分別為0.7370、0.6618。(2)20例女性樣本DNA無擴增產(chǎn)物。(3)同一尸體血液、器官組織檢測結果分型一致。(4)20例兩代家系觀察未見突變。 結論DYS713、DYS723基因座在山西漢族男性人群中均有較好的多態(tài)性,可用于法醫(yī)學的混合斑分析和父系親屬間的親權關系鑒定,對法醫(yī)學應用、人類遺傳學等研究均具有重要價值。
[Abstract]:Objective to obtain the frequency distribution of DYS713,DYS723 Y-STR loci in Shanxi Han population, and to provide data for population genetics, forensic individual identification and paternity identification. The feasibility of its application in forensic science was discussed. Methods DYS713,DYS723 Y-STR loci were obtained from GDB database, amplified by PCR and typed by electrophoresis. All alleles were sequenced to construct the allelic typing standard, and the alleles were named according to ISFG principle. Results (1) in the population samples of this study, 6 and 5 alleles were observed at the DYS713,DYS723 locus, and their genetic variations were 0.7370,0.6618,respectively. The individual recognition ability and the non-paternal exclusion rate were 0.7370 and 0.6618 respectively. (2) 20 female samples had no DNA amplification products. (3) the results of organ and tissue tests were consistent in the blood of the same cadaver. (4) No mutation was observed in 20 pedigree of two generations. Conclusion DYS713,DYS723 locus has good polymorphism in male population of Han nationality in Shanxi province. It can be used for mixed spot analysis of forensic science and paternity identification between paternal relatives. It is of great value for forensic application and human genetics research. [WT5 "HZ] conclusion [WT5" BZ] [WT5BZ] [WT5 "BZ]
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：D919

【引證文獻】

相關碩士學位論文 前1條

1 李松花;延邊地區(qū)朝鮮族和漢族男性人群六個Y染色體STR基因座的遺傳多態(tài)性[D];延邊大學;2011年

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本文編號：2456787