本文選題：濫用藥物 + 苯丙胺類��； 參考：《山西醫(yī)科大學(xué)》2004年碩士論文

【摘要】：目的 1、建立苯丙胺類為主的混合濫用藥物成分定性定量分析方法。2、建立生物樣品中AMP、MAMP及MDMA快速、靈敏檢測(cè)方法。3、觀察比對(duì)MDMA染毒家兔生命體征變化，并對(duì)急死與延緩死亡動(dòng)物體內(nèi)藥物分布進(jìn)行研究。 方法 1、薄層色譜掃描（TLCS）法：血漿樣品經(jīng)堿化，乙酸乙酯或環(huán)己烷萃取，薄層色譜掃描檢測(cè)，Rf值結(jié)合光譜掃描圖定性、外標(biāo)兩點(diǎn)法或標(biāo)準(zhǔn)曲線法定量分析。2、氣相色譜-質(zhì)譜聯(lián)用（GC-MS）檢測(cè)方法：體外檢材乙醇溶解后GC-MS全掃描（full scan）定性定量分析。生物樣品加入內(nèi)標(biāo)物SKF525A后堿化，乙醚萃取，TFA衍生化，GC-MS全掃描定性分析，選擇離子掃描（SIM）定量分析。3、動(dòng)物MDMA染毒與生命體征記錄方法：家兔以10mg/kg劑量灌胃，BL-5生物機(jī)能實(shí)驗(yàn)系統(tǒng)記錄從開始給藥到動(dòng)物被處死前的心電、血壓和呼吸等主要生命體征變化。4、樣品采集：動(dòng)物分別于給藥后2h和6h處死，迅速采集器官組織與體液檢材，冷凍保存待檢。 結(jié)果 1、11種濫用藥物在DB-5色譜柱上能有效分離，，GC-MS檢測(cè)線性關(guān)系良好。2、TLCS法對(duì)血漿中AMP與MDMA最低檢出限為0.2 μg/斑點(diǎn)(S/N≥3)，線性范圍為0.2～15 μg/斑點(diǎn)。GC-MS法對(duì)血漿與肝臟中AMP、MAMP及MDMA檢測(cè)線性范圍分別為0.02μg/ml～2 .0 μg/ml和0.05μg/g～5 .0 μg/g，最低檢出濃度分別為0.005 μg/ml和0.01 μg/g（S/N≥3）。3、家兔給藥后，四肢出現(xiàn)間歇性震顫，血壓升高，呼吸與心率加快，1h左右達(dá)到高峰，之后緩慢下降；給藥2h后體內(nèi)器官組織中藥物濃度高于體液，其中以胃內(nèi)容物、肺及脾含量最高，6h后組織與血液中藥物濃度明顯下降，而膽汁與尿液中藥物濃度基本不變，膽汁中藥物濃度高于其它組織。 結(jié)論 1、建立的混合濫用藥物分析方法可用于11種組分快速、準(zhǔn)確定性定量分析，為鑒別毒品、查證毒源及串并毒品案等禁毒、緝毒工作提供有力證據(jù)。2、TLCS與GC-MS分析方法分別適用于苯丙胺類中毒與濫用案例的快速鑒定。3、尿液為鑒定MDMA濫用的最佳檢材；對(duì)濫用MDMA死亡者臟器組織與體液中藥物含量測(cè)定，可對(duì)用藥后急死或延緩死亡做出判斷。
[Abstract]:Objective 1. To establish a qualitative and quantitative analysis method of amphetamine-based mixed drug abuse. 2. To establish a rapid and sensitive method for the detection of AMPMAMP and MDMA in biological samples, and to observe the changes of vital signs in rabbits exposed to MDMA. The distribution of drugs in animals with acute death and delayed death was studied. Methods 1. TLC scanning TLCSA: plasma samples were alkalized, extracted with ethyl acetate or cyclohexane, and their RF values were determined by TLC scanning and analyzed by spectral scanning. The external standard two-point method or standard curve method was used for quantitative analysis of .2. gas chromatography-mass spectrometry (GC-MS) was used to detect the quality and quantitative analysis of ethanol dissolved in vitro by GC-MS scanning. The biological samples were alkalized after adding the internal standard SKF525A, and then extracted by ether. The derivatization of TFAs was analyzed by GC-MS. The methods of animal MDMA exposure and vital signs recording were as follows: BL-5 biological function experiment system with 10mg/kg dose was used to record the electrocardiogram from the beginning of administration to the death of the animals. Changes of blood pressure and respiration. Sample collection: animals were killed at 2 h and 6 h after administration, organ tissue and body fluid samples were collected quickly and frozen to be preserved for examination. Results 1There was a good linear relationship between the determination of AMP and MDMA by GC-MS on DB-5 column. The lowest detection limit of AMP and MDMA in plasma was 0.2 渭 g / s / n 鈮

本文編號(hào)：1990622